FastQCFastQC Report
Fri 17 Jun 2016
SRR1526799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22498
Sequences flagged as poor quality0
Sequence length56
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5482.4357720686283226No Hit
ACCTGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3421.5201351231220552No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.9423059827540227No Hit
ACCTGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.5378255844963996No Hit
ACCTGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1010.44892879367054844No Hit
ACCTGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.40448039825762294No Hit
ACCTGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.36003200284469733No Hit
ACCTGAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG600.26669037247755356No Hit
ACCTGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.24891101431238333No Hit
ACCTGAGGGCAGACAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGCAGTGGA540.2400213352297982No Hit
ACCTGAGGGGTTTAATTTTGCATGTGATTTCTTTTTTCGTGCATCAATGAGGTTTA540.2400213352297982No Hit
ACCTGAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC530.23557649568850567No Hit
ACCTGAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA530.23557649568850567No Hit
ACCTGAGGGAAGTGGCTTTAATGACCCTGAAGGAATTTCTTCTGGTGATAGCTTTT480.21335229798204286No Hit
ACCTGAGGGGTGCTTCACGATACATACTATGTAGTAGCCCATTTCCACTATGTTCT450.2000177793581652No Hit
ACCTGAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.17779358165170236No Hit
ACCTGAGGGAGGGGCGGCAGAGTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.16001422348653213No Hit
ACCTGAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.16001422348653213No Hit
ACCTGAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.16001422348653213No Hit
ACCTGAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.1555693839452396No Hit
ACCTGAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.1422348653213619No Hit
ACCTGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.13779002578006933No Hit
ACCTGAGGGAATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.13779002578006933No Hit
ACCTGAGGGAAGCATGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT290.1289003466974842No Hit
ACCTGAGGGGCCATCTATGACATCTGTCGTAGAAACCTCGACATTGAGCGCCCAAC290.1289003466974842No Hit
ACCTGAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11112098853231399No Hit
ACCTGAGGGGAGTCCGAGCACGTTCTCGGCGCTAGCGGGGCTGAGGTTCCAGAGCT230.10223130944972886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAG309.089654E-750.70814550
AGGGGTT252.04201E-549.9175956
AGGGGTC402.5229383E-949.9175956
GGAGATG150.00837886949.9175958
ACAGTGT150.00837886949.91759510
ATCCCAA150.00837886949.91759524
AGGGGGT204.129645E-449.9175956
GGAGAGT355.0498784E-849.9175958
AGGGGGG150.00837886949.9175956
AGGGGCC204.129645E-449.9175956
AGGGGAT204.129645E-449.9175956
TGCATGG150.00837886949.91759513
GGGTCGA301.0137464E-649.9175958
GGCGTGA150.00837886949.9175959
GAAGTGT150.00837886949.9175959
GAAGTGG402.5229383E-949.9175959
AGGGCTG355.0498784E-849.9175956
AGGGCTA204.129645E-449.9175956
GGATTTG150.00837886949.9175958
GAGCTGG204.129645E-449.9175959
Produced by FastQC (version 0.11.5)