FastQCFastQC Report
Fri 17 Jun 2016
SRR1526798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52312
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGATGTGTGTAAAATTTTTATTTATAGCTTCCTTGCACAGCAATAAGTGATAGAAAG1090.20836519345465665No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.15866340419024316No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.15675179691084262No Hit
TGCGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.13572411683743693No Hit
TGCGGAGGGATGACAGAAAGGTGGAATAAACATTAAATTTGTGCATTCTTTGGTAATTTTTTTG680.12998929499923537No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC550.1051384003670286No Hit
TGCGGAGGGAGCAAGATGTTAAAAAAGAAAATCCTTTACAGTTCAAGTTTAGAGCTAAATTCTT530.10131518580822754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAA201.9031281E-458.54017656
CGGATGG150.004493979358.54017357
AGGGTTT201.9772486E-458.09036
GAGGGTA406.87578E-1058.09035
CGGAGAG201.9772486E-458.09033
AGGGTAC150.004633556658.0902986
GGGCAAT150.004633556658.0902987
AGGGGAT1150.058.0902986
AGGGCTA150.004633556658.0902986
AGGGAAC750.058.0902986
AGGGAAT351.5812475E-858.0902946
TGATAGA201.9866727E-458.03455455
GTGATAG201.9866727E-458.03455454
GCAATAA201.9866727E-458.03455447
CAATAAG201.9866727E-458.03455448
AGCGTAG150.00465122858.0345552
AAGCGTA150.00465122858.0345551
ATCCCTC150.00465122858.0345547
CAATGTC150.00465122858.0345555
ACTAGCT201.9961326E-457.97891237
Produced by FastQC (version 0.11.5)