FastQCFastQC Report
Fri 17 Jun 2016
SRR1526797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76255
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2030.266212051668743No Hit
TGCGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1960.2570323257491312No Hit
TGCGGAGGGATGTGTGTAAAATTTTTATTTATAGCTTCCTTGCACAGCAATAAGTGATAGAAAG1580.20719952789980986No Hit
TGCGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1340.17572618188971215No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1340.17572618188971215No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1320.17310340305553734No Hit
TGCGGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1290.16916923480427515No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1260.16523506655301293No Hit
TGCGGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1260.16523506655301293No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.1613008983017507No Hit
TGCGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1150.15080978296505146No Hit
TGCGGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1120.14687561471378927No Hit
TGCGGAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGAC1100.14425283587961446No Hit
TGCGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTCCTCCTCTCCCC1030.1350731099600026No Hit
TGCGGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG960.1258933840403908No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.1245819946233034No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT940.12327060520621598No Hit
TGCGGAGGGCAGCTTTCAAGGAAGGGATGGCCTTGGAGGACTATCAAAGGATGCTTGGCTACCA940.12327060520621598No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG900.11802504753786636No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGTACCCGACTGCTCTTCCGAAGGTCC890.11671365812077897No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG880.11540226870369157No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT880.11540226870369157No Hit
TGCGGAGGGGGAGCAGCTGGACAACGAGCGAGTTGGGATGGCTACCAGCTGGGGCAGCATCTTG870.11409087928660416No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC860.11277948986951675No Hit
TGCGGAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC860.11277948986951675No Hit
TGCGGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA850.11146810045242934No Hit
TGCGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAAAGCACGGACTGTTCTTCCAGAGGTCCT840.11015671103534194No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG790.10359976394990493No Hit
TGCGGAGGGATTTATATGAGGTATTATTGTGCCAGAACATATCTGCTGTGGAGTATGTAATATC790.10359976394990493No Hit
TGCGGAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT780.10228837453281753No Hit
TGCGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.10097698511573011No Hit
TGCGGAGGGATGACAGAAAGGTGGAATAAACATTAAATTTGTGCATTCTTTGGTAATTTTTTTG770.10097698511573011No Hit
TGCGGAGGGGGGAGGTGCCACCTGTCACAATAATGTGCTAAGATACCATAACTGGGGGCCGGTT770.10097698511573011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCAAT150.004644552758.08152852
CATGCGA150.004644552758.08152858
AGTATGT201.9845762E-458.08152852
TCGGACT150.004644552758.08152856
CCGACCA150.004644552758.08152853
CAATGTC201.9845762E-458.08152855
ATCGGGT150.004644552758.08152858
GACTGGG150.004644552758.08152855
TATGTAA201.9845762E-458.08152854
ACGACCT201.9845762E-458.08152858
AATGTCG201.9845762E-458.08152856
GTCGGAC150.004644552758.08152855
CGGACTT201.9845762E-458.08152857
TCGACGC258.5210995E-658.08152458
CATATCT201.9910424E-458.0433738
CTGCGTT201.9910424E-458.0433732
CGGTGCA201.9910424E-458.0433738
CGTTTGC201.9910424E-458.0433735
TGCGTTT201.9910424E-458.0433733
ATCTGCT201.9910424E-458.0433741
Produced by FastQC (version 0.11.5)