FastQCFastQC Report
Fri 17 Jun 2016
SRR1526796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76482
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1910.2497319630762794No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1830.23927198556523105No Hit
TGCGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1410.18435710363222718No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1400.18304960644334614No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1370.17912711487670302No Hit
TGCGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1360.17781961768782195No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.1608221542323684No Hit
TGCGGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1210.15820715985460632No Hit
TGCGGAGGGATGTGTGTAAAATTTTTATTTATAGCTTCCTTGCACAGCAATAAGTGATAGAAAG1190.1555921654768442No Hit
TGCGGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1170.15297717109908215No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1110.14513218796579586No Hit
TGCGGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1040.13597970764362857No Hit
TGCGGAGGGCAGCTTTCAAGGAAGGGATGGCCTTGGAGGACTATCAAAGGATGCTTGGCTACCA1000.13074971888810438No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT980.1281347245103423No Hit
TGCGGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG940.12290473575481813No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG910.118982244188175No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGTACCCGACTGCTCTTCCGAAGGTCC910.118982244188175No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.11767474699929395No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC890.11636724981041291No Hit
TGCGGAGGGATGACAGAAAGGTGGAATAAACATTAAATTTGTGCATTCTTTGGTAATTTTTTTG880.11505975262153187No Hit
TGCGGAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT880.11505975262153187No Hit
TGCGGAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGAC840.10982976386600769No Hit
TGCGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTCCTCCTCTCCCC840.10982976386600769No Hit
TGCGGAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC840.10982976386600769No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG830.10852226667712665No Hit
TGCGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.10852226667712665No Hit
TGCGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAAAGCACGGACTGTTCTTCCAGAGGTCCT830.10852226667712665No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG780.10198478073272144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTC258.249892E-658.3988142
ATTCGTT258.249892E-658.3988140
TGAGTTA258.249892E-658.3988140
TCTTAAG150.00454534858.39880839
CGACCGT150.00454534858.39880844
GACTTGA150.00454534858.39880840
CGTAGAA150.00454534858.39880843
TTGACGG150.00454534858.39880843
GTCGTAG150.00454534858.39880841
GCTAGGC150.00454534858.39880839
TCGTAGA150.00454534858.39880842
CGACTGT303.542773E-758.39880844
ATTGACT150.00454534858.39880842
ATGTTCC150.00454534858.39880843
GTGGAAT150.004569221358.32166721
AACTACC150.004569221358.32166722
GACCGCG258.314888E-658.32166325
AAATGCG201.9444535E-458.32166326
TCTTACC150.004641401658.0914558
TCGACGC351.5941623E-858.09144658
Produced by FastQC (version 0.11.5)