FastQCFastQC Report
Fri 17 Jun 2016
SRR1526795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69643
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2680.38481972344672116No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2270.3259480493373347No Hit
TGCGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1700.2441020633803828No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1540.22112775153281736No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1270.1823586002900507No Hit
TGCGGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1240.17805091681863217No Hit
TGCGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1160.16656376089484945No Hit
TGCGGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1140.16369197191390378No Hit
TGCGGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1110.15938428844248523No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1080.15507660497106673No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.15507660497106673No Hit
TGCGGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC1060.15220481599012106No Hit
TGCGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1000.14358944904728402No Hit
TGCGGAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA960.13784587108539265No Hit
TGCGGAGGGATGTGTGTAAAATTTTTATTTATAGCTTCCTTGCACAGCAATAAGTG900.1292305041425556No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG890.12779460965208275No Hit
TGCGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTCCTC880.12635871516160993No Hit
TGCGGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA860.12348692618066426No Hit
TGCGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.1220510316901914No Hit
TGCGGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT830.11917924270924572No Hit
TGCGGAGGGCAGCTTTCAAGGAAGGGATGGCCTTGGAGGACTATCAAAGGATGCTT820.11774334821877289No Hit
TGCGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA820.11774334821877289No Hit
TGCGGAGGGGAGCTGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAG810.11630745372830005No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA790.11343566474735438No Hit
TGCGGAGGGATGACAGAAAGGTGGAATAAACATTAAATTTGTGCATTCTTTGGTAA760.10912798127593584No Hit
TGCGGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT740.10625619229499017No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGTACCCGACTGCTCTTCC730.10482029780451733No Hit
TGCGGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT720.1033844033140445No Hit
TGCGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG700.1005126143330988No Hit
TGCGGAGGGGGAGCAGCTGGACAACGAGCGAGTTGGGATGGCTACCAGCTGGGGCA700.1005126143330988No Hit
TGCGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT700.1005126143330988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCA203.8917494E-450.67056348
AGTCCGT150.00796664750.67056348
AGGGTTA203.8917494E-450.67056350
CCCACTT150.00796664750.67056349
CCACTTT150.00796664750.67056350
AAATTAG150.00796664750.67056349
CAACCAA251.9101513E-550.6705649
ACTGTTG150.00819941150.30311647
CACCCAC150.00819941150.30311647
TGAGTCC204.0487343E-450.26666646
ATAATGC150.00827021750.1939241
ATGTGGA204.0778096E-450.1939240
TAGCCCT150.00827021750.1939244
TAGGCTC150.00827021750.1939241
TGGGATT150.00827021750.1939240
TATAATG150.00827021750.1939240
TGCACCC150.00827021750.1939245
GTCTGAA150.00831767350.12138739
GCTGGTA150.00831767350.12138739
CCGCTTG150.00831767350.12138739