FastQCFastQC Report
Fri 17 Jun 2016
SRR1526794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131635
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.3274205188589661No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.23701902989326545No Hit
TGATCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2510.19067877084362062No Hit
TGATCAGGGAGGACAAATCGGCTTCCTCTCCCCACCCTCCTGCCACTCTAAGTATTGAATGTAC1560.11850951494663273No Hit
TGATCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.1177498385687697No Hit
TGATCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.11471113305731757No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.10179663463364608No Hit
TGATCAGGGTGGTGAAAAGTCTGGTGTAATTCTGATAGGCCTTCCTTTATATGTTACTTGACCT1330.10103695825578304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGAC258.150884E-658.55773556
TTACGGG150.00450397558.55773556
ACGGTCC150.00450397558.55773558
AATCCTT150.004649923658.08820354
GATAACA150.004656960358.0660348
CTAAGTA501.8189894E-1258.0660348
AGGGTAC550.058.066036
TACGTAG202.0000702E-458.0217440
GTACGTA202.0038462E-457.9996239
AGTCCAG150.00468518657.97751636
CTAAGCG150.00468518657.97751634
AGTACGT150.00468518657.97751638
ATAGGCC202.0076279E-457.97751235
TAGCATA202.0114152E-457.95543330
AATAGGG150.004692262557.9554329
TACGAAT202.0190071E-457.9113111
AAATCGG550.057.91130415
GATGTAG150.004706438657.91130411
ATGTAGG150.004706438657.91130412
GTCAGCT150.004706438657.91130417
Produced by FastQC (version 0.11.5)