FastQCFastQC Report
Fri 17 Jun 2016
SRR1526793_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526793_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176954
Sequences flagged as poor quality0
Sequence length64
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.36506662748510915No Hit
TGATCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC5960.33681069656520907No Hit
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4070.2300032776879867No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3820.21587531222803666No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT3790.21417995637284265No Hit
TGATCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3340.18874961854493258No Hit
TGATCAGGGAGGACAAATCGGCTTCCTCTCCCCACCCTCCTGCCACTCTAAGTATTGAATGTAC3310.18705426268973857No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.17292629722978853No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2960.1672751110458085No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2530.14297501045469446No Hit
TGATCAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2460.13901918012590844No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2360.13336799394192841No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2330.1316726380867344No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2310.1305424008499384No Hit
TGATCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2260.12771680775794841No Hit
TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2110.11924002848197836No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2050.11584931677159034No Hit
TGATCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2010.11358884229799836No Hit
TGATCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.10737253749562033No Hit
TGATCAGGGTGGTGAAAAGTCTGGTGTAATTCTGATAGGCCTTCCTTTATATGTTACTTGACCT1840.10398182578523231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA201.977032E-458.16903356
ATATAAG150.00462703658.16903358
TTGCATA150.00462703658.16903353
CCTAAAC150.004637456558.13647
TAGTTCA150.004637456558.13648
AAATTCG201.9825935E-458.13648
GAATCCC150.00464267458.11950345
CCTTTAT453.092282E-1158.11950344
TACACTA150.00465835158.07005339
AGCGTAG351.6150807E-858.0700537
TCACGTC201.9937544E-458.0700539
CGATTTC150.004668824458.03713233
ATCAATT303.7268364E-758.03713232
TCAATTG303.7268364E-758.03713233
AGCGATA150.004668824458.03713234
GCGATAC150.004668824458.03713235
ATTGGGC303.7268364E-758.03713236
AACCTAC150.004668824458.03713235
ACGTATC150.004668824458.03713232
CGATACG150.004668824458.03713236
Produced by FastQC (version 0.11.5)