FastQCFastQC Report
Fri 17 Jun 2016
SRR1526792_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526792_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177225
Sequences flagged as poor quality0
Sequence length64
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.3599943574552123No Hit
TGATCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC5040.2843842573000423No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3810.21498095641134152No Hit
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3780.2132881929750317No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT3580.20200310339963326No Hit
TGATCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.19466779517562421No Hit
TGATCAGGGAGGACAAATCGGCTTCCTCTCCCCACCCTCCTGCCACTCTAAGTATTGAATGTAC3240.18281845112145578No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.1743546339399069No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2930.16532656227958809No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2530.14275638312879108No Hit
TGATCAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2350.13259980251093242No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2260.1275215122020031No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2110.11905769502045423No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2110.11905769502045423No Hit
TGATCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2030.11454365919029483No Hit
TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1970.11115813231767527No Hit
TGATCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1900.10720835096628578No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1880.10607984200874593No Hit
TGATCAGGGTGGTGAAAAGTCTGGTGTAATTCTGATAGGCCTTCCTTTATATGTTACTTGACCT1850.10438707857243618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAATAC201.9279755E-458.4652643
CCGATTG150.00453486858.46525243
TATGGGT150.00453486858.46525242
GCATGAT150.004540006658.44854439
TAATAAC150.004586447558.29854626
AACATTA150.004591629458.28192524
TGATTGA150.004591629458.28192524
CTACCGA150.004648918358.09974353
ATAGGCC150.004696185257.9515335
ACAGGCG150.004696185257.9515335
AGGATTT303.7650352E-757.9515335
GTAGGAT303.7724203E-757.9351133
CTAGTCT150.004701459357.9351133
CATCCTA150.004701459357.9351133
GATAGGT150.004701459357.9351134
ATCAATT150.004706737657.91869732
GCTAGTC150.004706737657.91869732
GTAGCGA150.00472259957.86951429
TAACTAC150.00472259957.86951429
TTATTAC150.004743809357.8040728
Produced by FastQC (version 0.11.5)