FastQCFastQC Report
Fri 17 Jun 2016
SRR1526791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159858
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7010.43851418133593567No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5370.33592313177945426No Hit
TGATCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4910.30714759348922166No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4220.2639842860538728No Hit
TGATCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3750.2345831925834178No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG3370.21081209573496476No Hit
TGATCAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2840.17765767118317508No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2780.1739043401018404No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2760.1726532297413955No Hit
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2720.1701510090205057No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2610.16326990203805877No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.15951657095672409No Hit
TGATCAGGGAGGACAAATCGGCTTCCTCTCCCCACCCTCCTGCCACTCTAAGTATT2270.14200102591049557No Hit
TGATCAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA2230.13949880518960578No Hit
TGATCAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2020.12636214640493437No Hit
TGATCAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1950.12198326014337725No Hit
TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG1930.12073214978293234No Hit
TGATCAGGGGTCTTTGATACTTTTCTGCCTAACTTGAACTTGCAGGTCACCTCTGA1920.12010659460270989No Hit
TGATCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1830.11447659798070789No Hit
TGATCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1800.11259993244004053No Hit
TGATCAGGGCGAGGAGCTGCGGGTGATTGAAGAGATTCCCCAGGCTGAGGACAAAC1760.11009771171915075No Hit
TGATCAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT1720.10759549099826095No Hit
TGATCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1640.10259104955648136No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.10196549437625894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAATC150.00802524250.6074449
CCTTGAA150.00803533550.5913648
ACCGTGC150.00830107250.17680746
AACTCAG150.00830107250.17680746
CCATTAG150.00831142250.16099542
CTCGTTT150.00832178150.145240
TAGGAAC150.00832178150.145238
ACAACGA204.1136463E-450.14519537
CGATACG150.00833214950.1294136
ACTATAG150.00834252850.11363234
CAACCTA150.00834252850.11363234
AGCGATA150.00834252850.11363234
TAGGACT150.00834252850.11363235
CAGCGAT150.00837372150.06635333
CTACTAT150.00837372150.06635332
CCTAGGA150.00837372150.06635333
TACTATA150.00837372150.06635333
ACTACTA150.00838413750.05061331
CGGTAGG150.00838413750.05061331
TAGCGAC150.00839456450.03488530
Produced by FastQC (version 0.11.5)