##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526791_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 159858 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.68814823155551 39.0 39.0 40.0 36.0 40.0 2 38.30126737479513 39.0 38.0 40.0 35.0 40.0 3 38.06002827509415 39.0 38.0 40.0 35.0 40.0 4 38.22871548499293 39.0 38.0 40.0 35.0 40.0 5 38.47800547986338 39.0 39.0 40.0 36.0 40.0 6 38.266348884635114 39.0 38.0 40.0 35.0 40.0 7 38.6962867044502 39.0 38.0 40.0 37.0 40.0 8 38.66153085863704 39.0 38.0 40.0 37.0 40.0 9 38.65097774274669 39.0 38.0 40.0 37.0 40.0 10 37.978706101665225 39.0 38.0 40.0 35.0 40.0 11 38.017215278559725 39.0 38.0 40.0 35.0 40.0 12 37.963211099851115 39.0 38.0 40.0 35.0 40.0 13 37.74735077381176 39.0 38.0 40.0 34.0 40.0 14 37.94671520974865 39.0 38.0 40.0 35.0 40.0 15 37.893299052909455 39.0 38.0 40.0 34.0 40.0 16 37.8194647749878 39.0 38.0 40.0 34.0 40.0 17 37.734877203518124 39.0 38.0 40.0 33.0 40.0 18 37.649795443456064 39.0 38.0 40.0 33.0 40.0 19 37.57352775588335 39.0 38.0 40.0 33.0 40.0 20 37.54588447246932 39.0 38.0 40.0 33.0 40.0 21 37.42224974665015 39.0 38.0 40.0 33.0 40.0 22 37.26967058264209 39.0 37.0 40.0 33.0 40.0 23 37.13293673134907 39.0 37.0 40.0 33.0 40.0 24 36.97867482390622 39.0 36.0 40.0 33.0 40.0 25 36.84144678402082 39.0 36.0 40.0 32.0 40.0 26 36.44032203580678 39.0 36.0 40.0 31.0 40.0 27 36.24609340789951 38.0 36.0 40.0 31.0 40.0 28 36.0132117254063 38.0 35.0 40.0 30.0 40.0 29 35.898128338900776 38.0 35.0 40.0 30.0 40.0 30 35.64952019917677 38.0 35.0 40.0 29.0 40.0 31 35.08204156188617 38.0 35.0 40.0 28.0 40.0 32 34.81542994407537 38.0 34.0 39.0 27.0 40.0 33 34.651090342679126 38.0 34.0 39.0 27.0 40.0 34 34.853419910170274 38.0 34.0 40.0 27.0 40.0 35 34.59612280899298 38.0 34.0 40.0 26.0 40.0 36 34.41402369603023 38.0 34.0 40.0 25.0 40.0 37 34.21275757234546 38.0 33.0 40.0 24.0 40.0 38 33.99966845575448 38.0 33.0 40.0 23.0 40.0 39 33.86572458056525 38.0 33.0 40.0 23.0 40.0 40 33.553222234733326 38.0 33.0 39.0 21.0 40.0 41 33.04099888651178 38.0 33.0 39.0 15.0 40.0 42 32.84213489471907 37.0 32.0 39.0 13.0 40.0 43 32.68378185639755 37.0 32.0 39.0 11.0 40.0 44 32.395795018078545 36.0 32.0 39.0 4.0 40.0 45 32.046128438989605 36.0 31.0 39.0 4.0 40.0 46 31.401049681592415 36.0 31.0 39.0 4.0 40.0 47 31.114076242665366 36.0 30.0 39.0 4.0 40.0 48 30.798727620763426 36.0 30.0 39.0 4.0 40.0 49 30.526335873087366 36.0 29.0 39.0 4.0 40.0 50 30.144228002352087 35.0 29.0 39.0 4.0 40.0 51 29.249815461221836 35.0 28.0 39.0 4.0 40.0 52 28.94567053259768 35.0 27.0 39.0 4.0 40.0 53 28.545371517221533 35.0 25.0 38.0 4.0 40.0 54 28.077731486694443 34.0 23.0 38.0 4.0 40.0 55 27.72758323011673 34.0 19.0 38.0 4.0 40.0 56 16.48057025610229 20.0 4.0 23.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.8486665244061413 1 2 -0.9061567761957292 1 3 -1.1196122451597432 1 4 -1.155012742771362 1 5 -1.1282072938547998 1 6 -1.2847607955498361 1 7 -0.6756242274059119 1 8 -1.1967727587298072 1 9 -0.9266051403882258 1 10 -0.8407244028122705 1 11 -0.6063763913010121 1 12 -0.6451132266877977 1 13 -0.15960127795950996 1 14 -0.7791879800235435 1 15 -0.7767515196213566 1 16 -0.8616127971674459 1 17 -0.31013534822309197 1 18 -0.7837716564562527 1 19 -0.47048759069102175 1 20 -0.481290845710987 1 21 -0.3238840685269082 1 22 -0.8561285924668383 1 23 -0.4587824834093084 1 24 -1.052157754467622 1 25 -1.5219625654488311 1 26 -0.5725418532777837 1 27 -1.1883465800265256 1 28 -0.6987738032168807 1 29 -0.7020987675593062 1 30 -0.5469463011614053 1 31 -0.4406743648091336 1 32 -0.042492533070749516 1 33 -0.8520619332397175 1 34 -0.9943245718787068 1 35 -1.1296041239696777 1 36 -0.3943181764603665 1 37 -0.4284329992048157 1 38 -0.7712984962764793 1 39 0.03632400197929542 1 40 -0.2184701355146359 1 41 0.7859516955163599 1 42 0.35338785806785467 1 43 1.264017305961545 1 44 0.8793172350949732 1 45 1.4038799064638923 1 46 1.4960396996708702 1 47 1.71551147495736 1 48 1.6732453784374002 1 49 1.1864839226430774 1 50 1.2418371007789943 1 51 1.4995990010427427 1 52 1.7150520900419863 1 53 1.6267381809980996 1 54 1.5936376259368643 1 55 1.7074244828969505 1 56 2.690136581034924 2 1 -0.09788227902448199 2 2 -0.37622474412880536 2 3 -0.5866739391297671 2 4 -0.18202563925096626 2 5 -0.3214366420597443 2 6 -0.4033996941207647 2 7 -0.17597278090887158 2 8 -0.08824191174479523 2 9 -0.2868787548880505 2 10 -0.5592238799820137 2 11 -0.6947347043020571 2 12 -0.3712808809227255 2 13 -0.3611523410476991 2 14 -0.3135640692532391 2 15 -0.19240157190438367 2 16 -0.28279613631000444 2 17 -0.3727442711927651 2 18 -0.4085102413290329 2 19 -0.4124098632598603 2 20 -0.6139590227761715 2 21 -0.33791334702115705 2 22 -0.30022932442919625 2 23 0.18120531721808675 2 24 -0.13755336269348817 2 25 -0.25457845948856317 2 26 0.6385508619540019 2 27 0.33766422513206606 2 28 0.44378457949486716 2 29 0.4741821665640771 2 30 0.09704986475006194 2 31 0.6474225330646917 2 32 0.5378926185500248 2 33 0.26766619502797084 2 34 0.4604941802997544 2 35 0.3211191245489715 2 36 0.2831391257355236 2 37 0.7505492245665337 2 38 0.4442382761180852 2 39 0.21456554955684481 2 40 1.003253461557513 2 41 0.714716073348356 2 42 -0.10234584705587935 2 43 1.0603313526677098 2 44 0.29853996078336564 2 45 0.026135047628059738 2 46 0.5168222022850237 2 47 0.32221241605181916 2 48 0.5787002407587707 2 49 0.544099816682813 2 50 0.6931703179278266 2 51 1.6400224935488446 2 52 1.0663068826526505 2 53 0.5531498226850999 2 54 1.3068652313742994 2 55 0.6191968774595153 2 56 0.05080231822558545 3 1 -0.0473420210948845 3 2 -0.29688525301692437 3 3 -0.371267874298816 3 4 -0.26097300767202114 3 5 -0.7242337839210222 3 6 -0.5297939081326177 3 7 -0.2584056843595519 3 8 -0.21664030839868076 3 9 -0.3239561337657122 3 10 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1.519982796143232 3 51 1.446618868592413 3 52 1.7547871893797335 3 53 1.9048838763623408 3 54 2.4015846458044123 3 55 2.3034509729631765 3 56 0.8159643956044604 4 1 -0.3254877164591221 4 2 -0.38297796824871 4 3 -0.3447778080736512 4 4 -0.5060061202548027 4 5 -0.28052517464949744 4 6 0.006629932926983884 4 7 0.019740011004685698 4 8 -0.011342295153646376 4 9 -0.0855455377392218 4 10 -0.24469791274604802 4 11 0.39362360869898794 4 12 0.07011856139166639 4 13 0.4298026955504213 4 14 -0.13680387406328265 4 15 0.1967584141534786 4 16 0.2642150174020941 4 17 0.19317591005505363 4 18 -0.09502993460194631 4 19 0.1718965152692391 4 20 0.14784822713669143 4 21 -0.17156618773221055 4 22 -0.16076435405624068 4 23 -0.36606725162123155 4 24 -0.2177206683749091 4 25 -0.05176388995214154 4 26 0.34798794804671473 4 27 -0.01616114956294723 4 28 -0.24844267738906467 4 29 0.05949063641420338 4 30 0.4464311822823035 4 31 1.0758819265816015 4 32 0.8515472020285912 4 33 0.42608376212452015 4 34 0.8666025804391779 4 35 0.5392700482157551 4 36 -0.010212216195462531 4 37 0.22057362331174346 4 38 0.5796948812069616 4 39 0.34758228012498904 4 40 0.05967555984960171 4 41 0.9978722253176855 4 42 0.7441163348890498 4 43 0.4626928026502881 4 44 0.9190523344327204 4 45 0.8807010985168731 4 46 1.0258410241741807 4 47 1.1459750511163023 4 48 0.8719208751261469 4 49 1.1798614060867862 4 50 1.0564066372028371 4 51 1.1684731732281755 4 52 0.8143898383863579 4 53 0.9048838763623408 4 54 0.7128429239501095 4 55 1.03192779415523 4 56 0.4119908856706864 5 1 -0.15803265496433028 5 2 -0.5537668135881688 5 3 -0.5143502728211402 5 4 -0.26777572876045497 5 5 -0.17253760876221946 5 6 -0.39270331030407846 5 7 0.019694959871650042 5 8 -0.006206465987546039 5 9 -0.05554148313724738 5 10 -0.1410803067632571 5 11 -0.6899011919497511 5 12 -0.31511367719912897 5 13 0.20463713263651329 5 14 -0.20843517559051605 5 15 0.07165141771251626 5 16 -0.13318102710842794 5 17 -0.41852837430769796 5 18 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1.7780422880300577 6 45 1.1295185062746782 6 46 1.484912069810985 6 47 1.6551429566891258 6 48 1.4996633628497094 6 49 2.2379773677032198 6 50 1.8853024339321216 6 51 2.4834256442828213 6 52 2.7656445124414084 6 53 2.5705594181022136 6 54 3.1947098429031193 6 55 2.895467912189197 6 56 1.8582674095252614 7 1 -0.07668344861512111 7 2 -0.023154568690209487 7 3 -0.12687861749233775 7 4 0.002915881216864591 7 5 -0.1726793321182285 7 6 0.036293288335372154 7 7 0.09084008458820136 7 8 0.14713820521426868 7 9 0.034027677860485994 7 10 -0.19847826594693174 7 11 0.09510447698448843 7 12 -0.05037444817418901 7 13 0.06574824374762755 7 14 -0.2126868762707872 7 15 0.19721831113655242 7 16 -0.659258124614098 7 17 -0.43085830628048427 7 18 -0.33431433489628404 7 19 -0.36489524337682155 7 20 -0.653798204062717 7 21 -0.1160566218749608 7 22 -0.01916207296720529 7 23 -0.3264701213784065 7 24 -0.02534576035430547 7 25 -0.42124512615522747 7 26 0.1802175286206662 7 27 -0.04025975147612115 7 28 0.5673225911907736 7 29 0.3842698858623237 7 30 0.73630425071498 7 31 0.9778318897898401 7 32 0.6135505132868673 7 33 0.8605024523379114 7 34 0.06868131480268147 7 35 0.7483852064203163 7 36 0.7875250906478044 7 37 0.7699205695957758 7 38 0.5597353521414732 7 39 0.830428122656258 7 40 0.53907217500781 7 41 0.5484149037577168 7 42 0.8874202348154654 7 43 0.18569684974373502 7 44 0.9900604286196213 7 45 0.38943914119531087 7 46 1.0716467636885305 7 47 1.0590545213149767 7 48 0.9968288957295286 7 49 1.7802109277939273 7 50 1.37226388517929 7 51 1.7328587508587852 7 52 1.730780566863178 7 53 1.0593632729774995 7 54 2.344511430204708 7 55 1.657939567517996 7 56 2.073828634015058 8 1 -0.7223183692500399 8 2 -0.6064879020235452 8 3 -1.170132585746309 8 4 -0.6324015791005948 8 5 -0.6919253638642573 8 6 -0.6321590926170089 8 7 -0.3786043598562401 8 8 -0.44294115986509297 8 9 -0.5596231157903091 8 10 -0.274073504042164 8 11 0.2774457468257623 8 12 -0.22973952753926596 8 13 0.3332085612079396 8 14 -0.30435354293744865 8 15 -0.11270232378408451 8 16 0.2256625103065346 8 17 0.13045121752903555 8 18 0.22024915716720983 8 19 -0.09981587829745564 8 20 0.09600338323886604 8 21 -0.18490582822416712 8 22 -0.15229699360212834 8 23 -0.25920821661649995 8 24 -0.09657591908446506 8 25 0.1347072547971493 8 26 0.09926514766829087 8 27 1.0367243755080082 8 28 0.22010036896855212 8 29 0.5997460763385192 8 30 0.7333280602387902 8 31 1.1714826834406296 8 32 0.43319337042972705 8 33 1.3751849920204577 8 34 1.4881257592471258 8 35 1.2303296508647605 8 36 1.514906043028752 8 37 1.5768650140402158 8 38 1.6617194791256011 8 39 1.1218963766245125 8 40 1.369230905166539 8 41 1.9081371259799411 8 42 1.6390075364027652 8 43 0.725180976727863 8 44 1.7440286825878815 8 45 1.8080899348461017 8 46 2.3692658113075815 8 47 1.5840545213149753 8 48 2.8952415941422274 8 49 2.936956959539959 8 50 2.973057535972938 8 51 2.7439698619698945 8 52 3.5129234240060363 8 53 2.91987914599337 8 54 2.9970907952840733 8 55 3.0468284564068853 8 56 3.0613286340150587 9 1 0.06774157080990051 9 2 0.09805755252190806 9 3 -0.14795476356848525 9 4 -0.344889091588108 9 5 -0.04452276646166098 9 6 0.3324762720183614 9 7 0.1175494862976052 9 8 0.13208381515988066 9 9 0.1649523087851179 9 10 0.2480543680857039 9 11 0.25686632624633887 9 12 0.6137669659672227 9 13 0.8340077620071398 9 14 0.6809611423772353 9 15 0.3591258480440871 9 16 0.7269112615552871 9 17 0.5369447240225469 9 18 0.48673267365072803 9 19 0.49019411171253324 9 20 0.5769224641579527 9 21 0.6066026632843204 9 22 0.9762244849193493 9 23 0.5816009741926891 9 24 0.6320454595369185 9 25 0.9455963656862636 9 26 0.772292120695262 9 27 1.1079531467367758 9 28 0.9840863829545654 9 29 0.9037920303844729 9 30 1.1306807075914378 9 31 1.7752289371868883 9 32 1.4837428209791739 9 33 1.3466135634490257 9 34 1.6975663186876844 9 35 1.4292806998158056 9 36 1.593177771300482 9 37 1.8258160629912652 9 38 2.0881930055991305 9 39 1.8633049680331055 9 40 2.042957178892813 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#Quality Count 4 106.0 5 9.0 6 17.0 7 37.0 8 84.0 9 96.0 10 130.0 11 139.0 12 167.0 13 219.0 14 317.0 15 451.0 16 581.0 17 678.0 18 894.0 19 1056.0 20 1048.0 21 1351.0 22 1392.0 23 1402.0 24 1863.0 25 2166.0 26 2164.0 27 2500.0 28 2840.0 29 3259.0 30 3860.0 31 4431.0 32 5430.0 33 6687.0 34 8705.0 35 11709.0 36 16275.0 37 23714.0 38 29988.0 39 24093.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.65281687497655 0.2896320484429932 0.03628220045290195 0.021268876127563213 8 99.40112266034205 0.47747482775236394 0.07133962039812514 0.050062891507456235 9 97.90001125999325 1.343692527117817 0.3127775901112237 0.4435186227777152 10 55.50989002739932 29.84648875877341 5.77387431345319 8.869746900374082 11 34.170326164470964 27.443105756358772 18.376934529394838 20.009633549775426 12 34.35549049781682 17.06577087164859 25.959288868871123 22.619449761663475 13 25.334359243828896 17.272204081121995 30.049794192345708 27.3436424827034 14 27.205394787874237 20.184163445057486 28.836217142714162 23.774224624354115 15 26.991454916238162 20.44251773448936 28.12308423726057 24.44294311201191 16 28.46526292084225 20.18228677951682 26.3321197562837 25.02033054335723 17 29.999124222747685 21.28388945188855 22.342954372005156 26.374031953358607 18 29.573121145016202 22.48745761863654 23.0967483641732 24.842672872174056 19 29.51369340289507 21.315167210899673 23.343217105180848 25.827922281024406 20 29.445066528209583 21.76570309714307 24.10341742932746 24.685812945319878 21 30.31690625430069 20.48630659710493 24.932752818126087 24.26403433046829 22 29.426115677663926 20.65020205432321 24.310950968984972 25.612731299027885 23 30.05917752004904 22.571282012786348 23.65849691601296 23.711043551151647 24 29.03492198546064 23.17601131116978 23.371204064013213 24.417862639356365 25 28.015267175572518 21.944062069828558 23.431985984232263 26.608684770366665 26 27.5299994366303 19.964570305408973 25.13755610223282 27.367874155727907 27 26.274804381846632 19.38716744913928 25.831611893583723 28.506416275430357 28 27.034863695288674 21.918348687053403 25.066539745364135 25.980247872293788 29 28.668738798581256 20.69082204313878 25.453383298449662 25.187055859830306 30 26.522049969897655 21.170103351394744 27.270845875978328 25.03700080272928 31 28.280457808342955 21.0324531901009 26.566186436423873 24.120902565132273 32 29.75652610441767 20.437374497991968 28.350903614457827 21.45519578313253 33 29.136017874626567 20.663143624632845 28.26048753546055 21.940350965280047 34 26.617616593321742 22.326153691624867 31.05095641365283 20.005273301400564 35 25.482635181812473 25.142875086353072 30.025120894303836 19.349368837530616 36 26.354280544351038 24.60134956836431 27.982181676530832 21.062188210753824 37 26.227201045469112 25.37147452611474 28.046091114140847 20.355233314275303 38 27.808891654566825 24.112940229928405 27.75232096949583 20.32584714600894 39 27.59769861980067 22.563586631873488 28.43902285660389 21.399691891721947 40 25.420326697037577 22.43273514384234 29.217739296770784 22.9291988623493 41 24.126602513672896 22.561378555110107 30.201836479095732 23.11018245212127 42 23.896033589535374 21.51593551514236 29.87555001605197 24.712480879270295 43 24.28305142382146 20.85920477587879 31.39208302371566 23.465660776584087 44 24.28652392947103 20.54471032745592 31.557304785894207 23.611460957178842 45 23.884395393957718 21.318693778819263 30.491722730365105 24.305188096857915 46 24.331562596492553 21.473807588332043 30.82090351689762 23.373726298277788 47 24.011196146911605 21.388675248698195 31.67450481005636 22.92562379433384 48 24.981551444663793 21.267242303641147 31.133592769427747 22.617613482267313 49 25.18128507472098 21.485591777539568 30.586417806923517 22.74670534081594 50 22.12407248498309 22.2054666599364 31.34243602039271 24.3280248346878 51 22.373824347595708 23.173047202720007 31.099041815693028 23.35408663399126 52 23.58267343912673 23.159289522667763 30.448307410795973 22.809729627409535 53 23.203806302611156 22.88388000555296 30.73627219607002 23.17604149576587 54 23.364218532391305 22.73210004515132 30.12737759858568 23.776303823871693 55 24.163336426826266 22.377317752230155 29.359141902940085 24.100203918003498 56 23.36727029432371 23.431020444231244 28.647171323793952 24.55453793765109 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 2.5 4 4.0 5 3.0 6 5.0 7 7.0 8 110.0 9 213.0 10 204.0 11 195.0 12 189.5 13 208.0 14 232.0 15 451.0 16 670.0 17 830.0 18 990.0 19 1038.0 20 1086.0 21 1369.5 22 1982.0 23 2311.0 24 2625.5 25 2940.0 26 3247.0 27 3554.0 28 3755.5 29 4051.5 30 4146.0 31 3515.5 32 2885.0 33 3045.0 34 3205.0 35 2791.5 36 2378.0 37 2473.0 38 2616.5 39 2665.0 40 2931.5 41 3198.0 42 3399.5 43 3601.0 44 3623.5 45 3646.0 46 4023.5 47 4768.5 48 5136.0 49 5316.5 50 5497.0 51 6190.5 52 6884.0 53 7260.5 54 8438.0 55 9239.0 56 10366.5 57 11494.0 58 12077.0 59 12660.0 60 12099.5 61 11539.0 62 10599.0 63 8708.0 64 7757.0 65 6994.5 66 6232.0 67 5514.5 68 4797.0 69 4147.5 70 3498.0 71 3030.0 72 2187.5 73 1813.0 74 1459.0 75 1105.0 76 943.5 77 782.0 78 609.0 79 328.0 80 220.0 81 175.5 82 131.0 83 91.0 84 51.0 85 36.0 86 21.0 87 14.5 88 6.0 89 4.0 90 2.0 91 0.0 92 1.5 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0369077556331244 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 6.255551802224474E-4 21 0.0 22 0.0 23 0.0 24 0.010008882883559158 25 0.023771096848453 26 0.06568329392335698 27 0.06755995946402432 28 0.11197437725981808 29 0.17515545046228528 30 0.25147318244942385 31 0.30652203830899927 32 0.3115264797507788 33 0.32904202479700734 34 0.3534386768256828 35 0.39284865317969697 36 0.43538640543482343 37 0.43476085025460093 38 0.47854971287017223 39 0.5148319133230742 40 0.5842685383277659 41 0.6049118592751066 42 0.6249296250422249 43 0.6630884910357943 44 0.6618373806753494 45 0.6943662500469167 46 0.7300228953195961 47 0.7706839820340552 48 0.8188517309111836 49 0.7932039685220634 50 0.8570105969047529 51 0.8313628345156325 52 0.8588872624454202 53 0.8657683694278672 54 1.6320734652003652 55 0.9139361183049958 56 0.8926672421774324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 159858.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.75705939020881 #Duplication Level Percentage of deduplicated Percentage of total 1 68.25113854266539 28.499668455754485 2 12.914968839884947 10.785822417395439 3 6.203559443911793 7.771272003903465 4 3.5369726749760306 5.907743122020793 5 2.2126677852348995 4.619725005942774 6 1.4845997123681687 3.719551101602672 7 1.015699904122723 2.9688848853357355 8 0.7685162991371045 2.5672784596329246 9 0.5333173537871524 2.0042787974327214 >10 2.838866251198466 20.391222209711117 >50 0.16928331735378715 4.774237135457718 >100 0.06741371045062321 5.215879092694767 >500 0.0029961649089165865 0.7744373131153899 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 701 0.43851418133593567 No Hit TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 537 0.33592313177945426 No Hit TGATCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 491 0.30714759348922166 No Hit TGATCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 422 0.2639842860538728 No Hit TGATCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 375 0.2345831925834178 No Hit TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 337 0.21081209573496476 No Hit TGATCAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 284 0.17765767118317508 No Hit TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 278 0.1739043401018404 No Hit TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 276 0.1726532297413955 No Hit TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 272 0.1701510090205057 No Hit TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 261 0.16326990203805877 No Hit TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 255 0.15951657095672409 No Hit TGATCAGGGAGGACAAATCGGCTTCCTCTCCCCACCCTCCTGCCACTCTAAGTATT 227 0.14200102591049557 No Hit TGATCAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA 223 0.13949880518960578 No Hit TGATCAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 202 0.12636214640493437 No Hit TGATCAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 195 0.12198326014337725 No Hit TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 193 0.12073214978293234 No Hit TGATCAGGGGTCTTTGATACTTTTCTGCCTAACTTGAACTTGCAGGTCACCTCTGA 192 0.12010659460270989 No Hit TGATCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT 183 0.11447659798070789 No Hit TGATCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 180 0.11259993244004053 No Hit TGATCAGGGCGAGGAGCTGCGGGTGATTGAAGAGATTCCCCAGGCTGAGGACAAAC 176 0.11009771171915075 No Hit TGATCAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT 172 0.10759549099826095 No Hit TGATCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 164 0.10259104955648136 No Hit TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 163 0.10196549437625894 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.005004441441779579 0.0 0.0 0.0 0.0 7 0.005004441441779579 0.0 0.0 0.0 0.0 8 0.005629996622002027 0.0 0.0 0.0 0.0 9 0.005629996622002027 0.0 0.0 0.0 0.0 10 0.011259993244004054 0.0 0.0 0.0 0.0 11 0.01939221058689587 0.0 0.0 0.0 0.0 12 0.02189443130778566 0.0 0.0 0.0 0.0 13 0.023771096848453 0.0 0.0 0.0 0.0 14 0.032528869371567265 0.0 0.0 0.0 0.0 15 0.03315442455178971 0.0 0.0 0.0 0.0 16 0.03628220045290195 0.0 0.0 0.0 0.0 17 0.03815886599356929 0.0 0.0 0.0 0.0 18 0.04191219707490398 0.0 0.0 0.0 0.0 19 0.04503997297601622 0.0 0.0 0.0 0.0 20 0.047542193696906 0.0 0.0 0.0 0.0 21 0.052546635138685585 0.0 0.0 0.0 0.0 22 0.059427742121132505 0.0 0.0 0.0 0.0 23 0.06505773874313453 0.0 0.0 0.0 0.0 24 0.0725644009058039 0.0 0.0 0.0 0.0 25 0.07569217680691614 0.0 0.0 0.0 0.0 26 0.07881995270802837 0.0 0.0 0.0 0.0 27 0.0844499493300304 0.0 0.0 0.0 0.0 28 0.09070550113225488 0.0 0.0 0.0 0.0 29 0.09445883221358956 0.0 0.0 0.0 0.0 30 0.09946327365536914 0.0 0.0 0.0 0.0 31 0.10259104955648138 0.0 0.0 0.0 0.0 32 0.10571882545759362 0.0 0.0 0.0 0.0 33 0.10884660135870586 0.0 0.0 0.0 0.0 34 0.11322548762026298 0.0 0.0 0.0 0.0 35 0.12573659122471192 0.0 0.0 0.0 0.0 36 0.12886436712582416 0.0 0.0 0.0 0.0 37 0.13261769820715885 0.0 0.0 0.0 0.0 38 0.13511991892804864 0.0 0.0 0.0 0.0 39 0.13824769482916088 0.0 0.0 0.0 0.0 40 0.14200102591049557 0.0 0.0 0.0 0.0 41 0.14575435699183026 0.0 0.0 0.0 0.0 42 0.15263546397427716 0.0 0.0 0.0 0.0 43 0.1582654605962792 0.0 0.0 0.0 0.0 44 0.16702323311939346 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATAATC 15 0.008025242 50.60744 49 CCTTGAA 15 0.008035335 50.59136 48 ACCGTGC 15 0.008301072 50.176807 46 AACTCAG 15 0.008301072 50.176807 46 CCATTAG 15 0.008311422 50.160995 42 CTCGTTT 15 0.008321781 50.1452 40 TAGGAAC 15 0.008321781 50.1452 38 ACAACGA 20 4.1136463E-4 50.145195 37 CGATACG 15 0.008332149 50.12941 36 ACTATAG 15 0.008342528 50.113632 34 CAACCTA 15 0.008342528 50.113632 34 AGCGATA 15 0.008342528 50.113632 34 TAGGACT 15 0.008342528 50.113632 35 CAGCGAT 15 0.008373721 50.066353 33 CTACTAT 15 0.008373721 50.066353 32 CCTAGGA 15 0.008373721 50.066353 33 TACTATA 15 0.008373721 50.066353 33 ACTACTA 15 0.008384137 50.050613 31 CGGTAGG 15 0.008384137 50.050613 31 TAGCGAC 15 0.008394564 50.034885 30 >>END_MODULE