FastQCFastQC Report
Fri 17 Jun 2016
SRR1526790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31044
Sequences flagged as poor quality0
Sequence length64
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5911.9037495168148435No Hit
GCGTCCGGGAGGTTACTCAGTTATTGCACCCAGTTCCTAATTAAGCTTCTGACAGATAATAGAT4381.4109006571318128No Hit
GCGTCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.4284241721427651No Hit
GCGTCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.38332689086457933No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.3543357814714599No Hit
GCGTCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.2544775157840485No Hit
GCGTCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.2383713438989821No Hit
GCGTCCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.17394665635871664No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.17394665635871664No Hit
GCGTCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.17072542198170337No Hit
GCGTCCGGGAAGAAAAGGTGGAAAAAGTGTTTTGTTTTGTTTTTTTAATTAATCAAATCTTTAG440.14173431258858393No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA420.13529184383455742No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.13207060945754412No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.12562814070351758No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAAA380.1224069063265043No Hit
GCGTCCGGGCAGGGTATAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.11918567194949105No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG350.11274320319546449No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.10952196881845123No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAAAAAAAAAA330.10630073444143795No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.10307950006442469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGA700.058.61928657
ATAATAG750.058.6192856
AATAGAT750.058.6192858
ACAGATA700.058.05016352
GACAGAT700.058.05016351
CCTAATT850.058.05016336
CTAATTA850.058.05016337
CGGAGAC150.00462840358.0501652
AGCTTCT800.058.0501644
TAAGCTT800.058.0501642
GGTCCGG201.9713324E-458.0501642
GCTGGGT201.9713324E-458.0501638
TGCTGTC150.00462840358.0501639
TGCTGGG201.9713324E-458.0501637
TCTACAC150.00462840358.0501641
GCTGTCG150.00462840358.0501640
ATTAAGC800.058.0501640
GACCGGG150.00462840358.0501643
TTAAGCT800.058.0501641
AGCCGGG201.9713324E-458.0501651
Produced by FastQC (version 0.11.5)