FastQCFastQC Report
Fri 17 Jun 2016
SRR1526789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36594
Sequences flagged as poor quality0
Sequence length64
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8812.4074984970213693No Hit
GCGTCCGGGAGGTTACTCAGTTATTGCACCCAGTTCCTAATTAAGCTTCTGACAGATAATAGAT6721.8363666174782751No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.5520030606110292No Hit
GCGTCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.4809531617205006No Hit
GCGTCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.33885336393944365No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC1110.30332841449417935Illumina Single End Adapter 2 (96% over 31bp)
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA940.2568727113734492No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG850.23227851560365087No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAAA790.215882385090452No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.20768431983385255No Hit
GCGTCCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.19402087773952015No Hit
GCGTCCTGTCCCCTGCCTGATACTTGGTGTATGCAAGAGTATTGCCCTCCGTCCGATATGCCCA710.19402087773952015No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.1858228124829207No Hit
GCGTCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.1858228124829207No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAAAAAAAAAA680.1858228124829207No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.18309012406405423No Hit
GCGTCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG610.16669399355085532No Hit
GCGTCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.16396130513198884No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.15303055145652292No Hit
GCGTCCGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCA530.14483248619992348No Hit
GCGTCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.13663442094332406No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.13116904410559108No Hit
GCGTCCGGGAGTATTCCGAGGTCTGTGTCCAGATGTTGATGCACATGCAGGATCAGCAACCCAA480.13116904410559108No Hit
GCGTCCGGGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.12570366726785812No Hit
GCGTCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.12570366726785812No Hit
GCGTCCGGGAAGAAAAGGTGGAAAAAGTGTTTTGTTTTGTTTTTTTAATTAATCAAATCTTTAG460.12570366726785812No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA460.12570366726785812Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG430.11750560201125868No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG420.1147729135923922No Hit
GCGTCCGGGAGGTGACTTAATGTATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAAT410.11204022517352571No Hit
GCGTCCGGGAACCCAAACAGACAACGACAGACCACCAAGACATTAAAGGTTTTAAATTCTTCAA400.10930753675465923No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT400.10930753675465923No Hit
GCGTCCGGGGATCGCTTGCTGAAGATTCTCTCTGCGTTTGCTGTCGCTGCAGCAGCGGACGACC390.10657484833579275No Hit
GCGTCCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.1011094714980598No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG370.1011094714980598Illumina Single End Adapter 2 (100% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGATA950.058.3006952
GACAGAT950.058.3006951
GATAATA950.058.3006955
TGATCAT150.004556529758.3006951
CGGACGA150.004556529758.3006956
CTGACAG950.058.3006949
ATAATAG950.058.3006956
AGCGGAC150.004556529758.3006954
TCATGGC150.004556529758.3006954
AGGCCAA150.004556529758.3006947
TCTGACA950.058.3006948
GATCATG150.004556529758.3006952
CAGATAA950.058.3006953
TAATAGA950.058.3006957
CTTCTGA950.058.3006946
AGATAAT950.058.3006954
TAGGCCA150.004556529758.3006946
ATAGGCC150.00458151458.22038745
GATAGGC258.3134E-658.22038744
TAAGCTT950.058.14030542
Produced by FastQC (version 0.11.5)