FastQCFastQC Report
Fri 17 Jun 2016
SRR1526788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36450
Sequences flagged as poor quality0
Sequence length64
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8422.310013717421125No Hit
GCGTCCGGGAGGTTACTCAGTTATTGCACCCAGTTCCTAATTAAGCTTCTGACAGATAATAGAT6721.8436213991769548No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.4746227709190672No Hit
GCGTCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.4609053497942387No Hit
GCGTCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.3292181069958848No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC1040.28532235939643347Illumina Single End Adapter 2 (96% over 31bp)
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA810.2222222222222222No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG750.205761316872428No Hit
GCGTCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.19478737997256515No Hit
GCGTCCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.19204389574759945No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.18930041152263374No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.1838134430727023No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAAAAAAAAAA660.18106995884773663No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAAA650.17832647462277093No Hit
GCGTCCTGTCCCCTGCCTGATACTTGGTGTATGCAAGAGTATTGCCCTCCGTCCGATATGCCCA630.17283950617283952No Hit
GCGTCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.1700960219478738No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.1673525377229081No Hit
GCGTCCGGGAGTATTCCGAGGTCTGTGTCCAGATGTTGATGCACATGCAGGATCAGCAACCCAA610.1673525377229081No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.1673525377229081No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.16186556927297668No Hit
GCGTCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG550.15089163237311387No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG550.15089163237311387No Hit
GCGTCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.15089163237311387No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT540.14814814814814814No Hit
GCGTCCGGGAAGAAAAGGTGGAAAAAGTGTTTTGTTTTGTTTTTTTAATTAATCAAATCTTTAG490.13443072702331962No Hit
GCGTCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG490.13443072702331962No Hit
GCGTCCGGGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.11796982167352538No Hit
GCGTCCGGGAACCCAAACAGACAACGACAGACCACCAAGACATTAAAGGTTTTAAATTCTTCAA400.10973936899862827No Hit
GCGTCCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.10973936899862827No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA380.10425240054869685Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.10150891632373113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCC351.4619218E-858.5829945
AGCTTCT600.058.58298544
TAAGCTT600.058.58298542
TAGAGTT150.00446981358.58298544
ATGCAGG150.00446981358.58298545
GCTTCTG600.058.58298545
CATGCAG150.00446981358.58298544
GATAGGC406.2755134E-1058.5829844
TGCACAT150.00451948458.4200340
TACGTAG150.00451948458.4200340
ATGCACA150.00451948458.4200339
GCACATG150.00451948458.4200341
GTACGTA150.00451948458.4200339
TGTCTCC150.004569565458.25797726
TTATTGC650.058.25797721
TATTGCA650.058.25797722
CGCGATC150.004569565458.25797725
ACGCGAT201.9411907E-458.2579724
GCAACCC150.004594760558.17728856
AGCAACC150.004594760558.17728855
Produced by FastQC (version 0.11.5)