SRR1526787_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526787_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31646
Sequences flagged as poor quality	0
Sequence length	56
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	941	2.973519560133982	No Hit
GCGTCCGGGAGGTTACTCAGTTATTGCACCCAGTTCCTAATTAAGCTTCTGACAGA	586	1.8517348164064968	No Hit
GCGTCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	203	0.6414712759906466	No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	132	0.4171143272451495	No Hit
GCGTCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116	0.36655501485179803	No Hit
GCGTCCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76	0.24015673386841938	No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAA	71	0.22435694874549705	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	69	0.21803703469632815	Illumina Single End Adapter 2 (95% over 23bp)
GCGTCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	68	0.2148770776717437	No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAA	67	0.21171712064715922	No Hit
GCGTCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65	0.20539720659799027	No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	61	0.1927573784996524	No Hit
GCGTCCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	60	0.18959742147506795	No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	60	0.18959742147506795	No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	58	0.18327750742589902	No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.1674777223029767	No Hit
GCGTCCGGGAGGTGACTTAATGTATCTTAATAACTACTATAGGAACAAAGGAAGTG	53	0.1674777223029767	No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	52	0.16431776527839223	No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAA	49	0.1548378942046388	No Hit
GCGTCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49	0.1548378942046388	No Hit
GCGTCCTGTCCCCTGCCTGATACTTGGTGTATGCAAGAGTATTGCCCTCCGTCCGA	48	0.15167793718005435	No Hit
GCGTCCGGGAGTATTCCGAGGTCTGTGTCCAGATGTTGATGCACATGCAGGATCAG	47	0.14851798015546988	No Hit
GCGTCCGGGAAGAAAAGGTGGAAAAAGTGTTTTGTTTTGTTTTTTTAATTAATCAA	47	0.14851798015546988	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	44	0.1390381090817165	Illumina Single End Adapter 2 (96% over 30bp)
GCGTCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	43	0.13587815205713202	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40	0.12639828098337863	No Hit
GCGTCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39	0.12323832395879417	No Hit
GCGTCCGGGGTATGCTTCCAGAACCTGCGGGAGGTGGGCAATGCTGTTCTCTTCTG	37	0.11691840990962522	No Hit
GCGTCCGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG	36	0.11375845288504076	No Hit
GCGTCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.11059849586045628	No Hit
GCGTCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC	35	0.11059849586045628	No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34	0.10743853883587184	No Hit
GCGTCCGGGACCCCGATGAATATCATGGCGAGTACCCAGACGACTACCCTCGGGAG	33	0.10427858181128738	No Hit
GCGTCCGGGCATAGCTAGAGTGTGCTTATTTTGAGTTTTGATTCTATTTATTTTTA	33	0.10427858181128738	No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	32	0.1011186247867029	No Hit
GCGTCCGGGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.1011186247867029	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACAGA	50	5.456968E-12	50.71452	50
AGGCCAA	20	3.997917E-4	50.3088	47
TAGGCCA	20	3.997917E-4	50.3088	46
AGCTTCT	55	0.0	50.228436	44
TAAGCTT	55	0.0	50.228436	42
AATTAAG	55	0.0	50.228436	39
ATTGCGG	15	0.008205892	50.228436	40
ATTAAGC	55	0.0	50.228436	40
TAATTAA	55	0.0	50.228436	38
ATAGGCC	20	4.02968E-4	50.22843	45
TCCTAAT	55	0.0	50.148327	35
CCTAATT	55	0.0	50.148327	36
GTTCCTA	55	0.0	50.148327	33
TTCCTAA	60	0.0	50.148323	34
TGCAGTT	15	0.008257949	50.148323	32
CAGTTCC	55	0.0	50.06847	31
GTGCAGA	15	0.008310251	50.06847	31
GGAATGT	15	0.008310251	50.06847	31
CCAGTTC	55	0.0	49.988873	30
ACTTTCT	15	0.008362799	49.988873	30

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content