##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526787_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31646 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.47772230297668 39.0 38.0 40.0 36.0 40.0 2 38.16093661126209 39.0 38.0 40.0 35.0 40.0 3 38.214118687985845 39.0 38.0 40.0 35.0 40.0 4 37.965904063704734 39.0 38.0 40.0 35.0 40.0 5 38.44744991468116 39.0 38.0 40.0 36.0 40.0 6 38.42191746192252 39.0 38.0 40.0 36.0 40.0 7 38.33821020034127 39.0 38.0 40.0 36.0 40.0 8 38.285059723187764 39.0 38.0 40.0 36.0 40.0 9 38.22287176894394 39.0 38.0 40.0 35.0 40.0 10 37.482936232067246 39.0 38.0 40.0 33.0 40.0 11 37.6193831763888 39.0 38.0 40.0 34.0 40.0 12 37.49778803008279 39.0 37.0 40.0 33.0 40.0 13 37.12516589774379 39.0 37.0 40.0 33.0 40.0 14 37.42065347911269 39.0 37.0 40.0 33.0 40.0 15 37.372116539215064 39.0 37.0 40.0 33.0 40.0 16 37.1783163748973 39.0 37.0 40.0 33.0 40.0 17 37.26663717373444 39.0 37.0 40.0 33.0 40.0 18 37.142166466536054 39.0 37.0 40.0 33.0 40.0 19 36.7460026543639 38.0 36.0 40.0 32.0 40.0 20 36.991878910446815 39.0 36.0 40.0 33.0 40.0 21 36.88450357075144 39.0 36.0 40.0 33.0 40.0 22 36.713202300448714 38.0 36.0 40.0 32.0 40.0 23 36.52234089616381 38.0 36.0 40.0 31.0 40.0 24 36.45263224420148 38.0 36.0 40.0 31.0 40.0 25 36.39758579283322 38.0 36.0 40.0 31.0 40.0 26 36.119130379826835 38.0 36.0 39.0 31.0 40.0 27 36.064652720723 38.0 36.0 39.0 31.0 40.0 28 35.670669278897805 38.0 35.0 39.0 29.0 40.0 29 35.811097769070344 38.0 35.0 39.0 30.0 40.0 30 35.38099601845415 38.0 35.0 39.0 29.0 40.0 31 35.09985464197687 38.0 35.0 39.0 28.0 40.0 32 34.575301775895845 38.0 34.0 39.0 25.0 40.0 33 34.81719648612779 38.0 35.0 39.0 27.0 40.0 34 34.91524995260065 38.0 35.0 39.0 27.0 40.0 35 34.58923718637426 38.0 35.0 39.0 26.0 40.0 36 34.556689629021044 39.0 35.0 40.0 23.0 40.0 37 34.44084560449978 39.0 35.0 40.0 23.0 40.0 38 34.20517600960627 39.0 35.0 39.0 22.0 40.0 39 34.1532263161221 39.0 35.0 39.0 21.0 40.0 40 33.9376224483347 38.0 35.0 39.0 19.0 40.0 41 33.817322884408775 39.0 35.0 39.0 15.0 40.0 42 33.66564494722872 39.0 34.0 39.0 13.0 40.0 43 33.53842507741895 39.0 34.0 39.0 10.0 40.0 44 33.31504771535107 38.0 33.0 39.0 4.0 40.0 45 33.06724388548316 38.0 33.0 39.0 4.0 40.0 46 32.61217847437275 38.0 33.0 39.0 4.0 40.0 47 32.40700246476648 38.0 33.0 39.0 4.0 40.0 48 32.26041205839601 38.0 32.0 39.0 4.0 40.0 49 32.03874107312141 38.0 32.0 39.0 4.0 40.0 50 31.6965809264994 38.0 32.0 39.0 4.0 40.0 51 31.11072489414144 38.0 31.0 39.0 4.0 40.0 52 30.814131327813943 37.0 31.0 39.0 4.0 40.0 53 30.60013903810908 37.0 30.0 39.0 4.0 40.0 54 30.002559565189912 36.0 29.0 39.0 4.0 40.0 55 29.649307969411616 36.0 29.0 39.0 4.0 40.0 56 17.746381849206852 21.0 4.0 25.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.0323393692572367 1 2 -0.9287897284878994 1 3 -0.9121735043838441 1 4 -1.1941601954491006 1 5 -1.2123655622266867 1 6 -0.9448828673570873 1 7 -0.9453711898394417 1 8 -1.4457604524123013 1 9 -1.7061204022762055 1 10 -0.46247158795534205 1 11 -0.5368605407569191 1 12 -1.1475468437100176 1 13 0.19909746514128557 1 14 -0.5676116348737352 1 15 -0.12263065123590167 1 16 -0.0936706361340569 1 17 -0.8937053472457279 1 18 0.7465113417111624 1 19 0.22513336909534587 1 20 0.032722842949141295 1 21 -0.5594356615815883 1 22 -0.03404817197431953 1 23 0.24946863760334992 1 24 0.6693449098112509 1 25 -0.5340720221728645 1 26 -2.294097475058166 1 27 -2.1084459073740547 1 28 -2.2468287787150487 1 29 -1.0270524960096026 1 30 -1.1759797450017544 1 31 -1.58222593507503 1 32 -2.7621675276669 1 33 -1.474879501091209 1 34 -2.6399461891014013 1 35 -4.556530664731593 1 36 -2.978000200458389 1 37 -1.5839552998549564 1 38 -2.2577548830611995 1 39 -1.483799266353735 1 40 -2.0950125163254896 1 41 -0.2740619569874596 1 42 -1.2261669776987745 1 43 -1.6644310858842601 1 44 -1.188910796120929 1 45 -1.0704011288906479 1 46 -0.9589771903647524 1 47 -1.2801968512950594 1 48 0.24086242353723009 1 49 0.5273757555264069 1 50 -0.19913784595734896 1 51 -0.6447418488033492 1 52 -1.008801892526236 1 53 -1.079867170908841 1 54 -0.16043157323287716 1 55 -0.8472452392356757 1 56 0.2398547979557648 2 1 0.4248352692299662 2 2 -0.232460473760419 2 3 0.26580202398100994 2 4 -0.1162959017894849 2 5 0.12912498282337026 2 6 0.34877675444491274 2 7 0.35496251427624514 2 8 0.15268226171450294 2 9 0.5875392195257945 2 10 0.023624073891156172 2 11 -0.18202183107950276 2 12 0.1894943131309148 2 13 0.18352460640936386 2 14 -0.45526458259342917 2 15 -0.26501107392778067 2 16 0.7127809767691673 2 17 0.24978742249843577 2 18 0.7943422649592122 2 19 0.9737429352800007 2 20 -0.25092565538233913 2 21 0.7776054952593441 2 22 0.5710686244661716 2 23 0.5898468356011222 2 24 0.40905569957765664 2 25 0.1522461090840892 2 26 -0.2507159400192407 2 27 0.22303351420548267 2 28 -0.07775202676844373 2 29 0.8939708632784971 2 30 -0.05139687514635938 2 31 1.9394648324444361 2 32 0.9630827504198649 2 33 2.255932512256962 2 34 1.7705098731900293 2 35 2.7771734509525032 2 36 2.935236729463746 2 37 2.406033576674524 2 38 1.9491416686629393 2 39 1.7431195323114466 2 40 2.731461343518781 2 41 0.5357266970726045 2 42 1.3344559366504996 2 43 1.1920761443715762 2 44 0.9401214619435905 2 45 1.4868847443017899 2 46 2.848586769590753 2 47 1.3371557627205135 2 48 1.44887132231365 2 49 1.4105793150369763 2 50 2.559482843697822 2 51 2.885847695134359 2 52 2.4995407103658707 2 53 2.4740816611267533 2 54 1.3801690941753542 2 55 0.08935097878434561 2 56 0.5346267668100495 3 1 -0.7564773002917207 3 2 -0.23913455607410583 3 3 -0.808725228521773 3 4 -0.1251946782077269 3 5 -0.5227103898128931 3 6 -0.39315872942605523 3 7 0.1580770860226295 3 8 0.07148092689804031 3 9 0.29387959772379446 3 10 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2.9414650477483733 3 52 2.1291291419565255 3 53 1.2649604152980523 3 54 2.943016702629194 3 55 3.3251685538677727 3 56 2.619165142783352 4 1 -0.10130488649861746 4 2 -0.13568628021203466 4 3 -0.49838040093556657 4 4 -1.1941601954491006 4 5 -0.5227103898128931 4 6 -0.6345380397708809 4 7 -0.15226774156357692 4 8 -0.10093286620540454 4 9 -0.2578445402072447 4 10 -1.014195725886374 4 11 -0.2954812304120935 4 12 0.26624625973826 4 13 -0.8353852934794048 4 14 -0.08485301418408397 4 15 0.015300383246859894 4 16 0.32012246731422067 4 17 -0.41094672655606956 4 18 -0.39141969277159916 4 19 0.052719575991901024 4 20 -0.03624267429223238 4 21 -0.3180563512367627 4 22 -0.6547378271467323 4 23 -0.09535894860354688 4 24 0.496931116707799 4 25 -0.8444168497590709 4 26 -0.12168368195472112 4 27 0.20189892021215172 4 28 -0.5571736063012551 4 29 -0.2684318063544282 4 30 0.23781335844652318 4 31 -0.4098121419715852 4 32 1.1343841964710322 4 33 -0.8541898459187891 4 34 0.4979848453813531 4 35 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1.2147756713225846 119 34 1.0152262246917019 119 35 0.5814003697511652 119 36 1.2978618685071268 119 37 0.9332860794553852 119 38 0.4663830479732809 119 39 0.8265455612324715 119 40 -0.19846079218756074 119 41 0.7949035602539141 119 42 1.2910744016115743 119 43 0.8183275348053911 119 44 0.6731581693963093 119 45 1.0330471469714197 119 46 2.0755055682559345 119 47 2.306010045256663 119 48 2.0684486304337852 119 49 2.0101343762160653 119 50 2.835344912663338 119 51 4.148361599472516 119 52 4.198094659197906 119 53 5.161512139435985 119 54 4.425775323318845 119 55 4.359651312488463 119 56 3.7915789358868004 120 1 0.17886752729448574 120 2 0.5205637197879653 120 3 0.3776971852713302 120 4 0.16898635627503467 120 5 -0.7005121139508219 120 6 -0.01815872942605523 120 7 -0.19644877604633848 120 8 -0.16989838344678532 120 9 0.2518537356548265 120 10 0.13451117066534835 120 11 1.0881394592430809 120 12 0.4828410873244664 120 13 0.5827181547964599 120 14 0.6112676754710904 120 15 0.9204727970399631 120 16 0.4688293638659431 120 17 -0.013317416211243938 120 18 0.42539065205598803 120 19 1.172331644957417 120 20 0.6049211188112125 120 21 -0.9602977305471043 120 22 0.03276217285326766 120 23 0.7128307065688659 120 24 -0.5838878488094394 120 25 0.760109012309897 120 26 0.0033163180452788765 120 27 -1.0330148728912931 120 28 -2.157389123542636 120 29 -2.2824404270440866 120 30 -2.557445262243135 120 31 -1.5585190385233076 120 32 -2.364538217322071 120 33 -2.397293294194654 120 34 -2.559127223584163 120 35 -1.6028668716281445 120 36 -3.104077786665286 120 37 -2.2078777136480596 120 38 -1.7071083313370607 120 39 -2.1853078870433933 120 40 -2.003417688739283 120 41 -1.2848378190564276 120 42 -0.4427618052849809 120 43 -2.044819016918744 120 44 -1.657660796120929 120 45 -0.9637200944078899 120 46 -0.6917358110544072 120 47 -1.2737313340536787 120 48 -0.6599996454282859 120 49 -1.1579690720597995 120 50 -1.2217671563021781 120 51 -0.4206039177688652 120 52 -0.7544915476986489 120 53 -0.5249102743571186 120 54 -2.24987122840529 120 55 -1.6112538599253305 120 56 -0.8786796848028544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 21.0 5 1.0 6 8.0 7 8.0 8 34.0 9 34.0 10 42.0 11 68.0 12 74.0 13 46.0 14 104.0 15 111.0 16 114.0 17 140.0 18 165.0 19 186.0 20 226.0 21 265.0 22 286.0 23 258.0 24 355.0 25 341.0 26 300.0 27 368.0 28 445.0 29 462.0 30 638.0 31 784.0 32 1010.0 33 1243.0 34 1627.0 35 2085.0 36 2830.0 37 4941.0 38 6738.0 39 5288.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 98.98881375213297 0.42027428426973396 0.4645136826139164 0.12639828098337863 8 98.85242792109257 0.7429185634800203 0.24658573596358116 0.1580677794638341 9 94.5869936168868 2.8882007204702016 1.1628641850470833 1.3619414775959047 10 26.442520381722805 50.815268912342795 8.048410541616633 14.693800164317766 11 24.445427542185428 39.98925614611642 20.47968147633192 15.085634835366237 12 48.43582127283069 16.292738418757505 21.0737533969538 14.197686911458005 13 22.397775390254694 18.6121468748025 27.56114516842571 31.428932566517094 14 26.164444163559374 30.37982683435505 22.015420590279973 21.4403084118056 15 36.055109650508754 22.612652467926438 21.133792580420906 20.198445301143906 16 22.442014788598875 24.60342539341465 30.503065158313845 22.451494659672626 17 33.90001895974215 21.89218226632118 19.43057574416988 24.777223029766795 18 31.097137078935727 23.31416292738419 21.373949314289327 24.214750679390757 19 22.64425203817228 27.181950325475572 28.02881880806421 22.144978828287933 20 25.030810554589983 31.730131142360563 19.358508453152158 23.8805498498973 21 23.26676357201542 21.247551033305946 32.13676294002401 23.348922454654616 22 24.543386209947545 23.977753902546926 21.070593439929215 30.408266447576317 23 24.954180623143525 36.383745181065535 20.277444226758515 18.38462996903242 24 23.758809215308283 35.19577789716525 23.11727712290238 17.928135764624088 25 21.991465149359886 25.348506401137982 25.032400821874507 27.627627627627625 26 29.214762341481926 23.493880648935832 24.496378988646786 22.794978020935456 27 19.131781965347162 22.258758062476286 27.921461995700014 30.687997976476538 28 20.555397412784263 33.681247430179965 28.244298953094855 17.51905620394092 29 26.892272626547214 24.46104656684289 28.319984804837127 20.326696001772767 30 17.350657581999684 33.015369988908255 30.87307875138647 18.760893677705592 31 21.85657218946167 28.806036395916557 33.49819288567624 15.839198528945534 32 14.59911866341185 29.74669498779444 42.58948102590115 13.064705322892559 33 21.08834908352889 25.994165028223502 36.452717701528506 16.4647681867191 34 17.893501633313246 27.693381116995972 39.545209476388315 14.867907773302463 35 12.91059697229363 33.48249706433082 40.02031165698689 13.58659430638865 36 14.146031746031746 29.625396825396827 38.80952380952381 17.41904761904762 37 12.7230583603226 27.35124150631866 44.13221566012574 15.793484473232997 38 14.765868225427283 28.537391193849672 45.40313870004448 11.293601880678569 39 13.649219817586678 29.05583627292084 42.495312549655196 14.79963135983729 40 13.633182772440994 30.07824925249698 44.95833068261339 11.33023729244863 41 14.697928928196482 28.266471542646265 45.15954570037859 11.876053828778673 42 14.310262529832935 26.63802704852824 45.23786793953858 13.813842482100238 43 16.42309039386124 28.99353647276085 43.85328111567485 10.73009201770306 44 13.9473432873834 29.715704689440003 44.79322530323772 11.543726719938876 45 14.735099337748345 30.145185939887924 43.69268976057056 11.427024961793174 46 12.784326218540937 28.18732080280344 43.93118827652118 15.097164702134439 47 13.432122370936902 30.347355003186742 45.038240917782026 11.182281708094328 48 15.635262234975292 28.681651522397576 44.9705085286147 10.712577714012435 49 15.536146882570446 29.277699859747546 41.67410429682519 13.512048960856815 50 14.137645107794361 29.11723434111494 45.20665901262916 11.538461538461538 51 14.036989795918368 31.058673469387756 43.02933673469388 11.875 52 12.920613657385257 33.30781743373839 42.02787612030747 11.743692788568877 53 12.91742416993589 30.494689503396806 42.60517334864287 13.98271297802443 54 13.664276757869015 33.10613124135936 41.54261646786483 11.686975532906793 55 14.912896432901537 31.925212175355753 40.93548592942378 12.226405462318933 56 14.56146507992215 33.79063905816291 40.174839677120886 11.473056184794054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.0 9 6.0 10 11.0 11 16.0 12 17.5 13 104.0 14 189.0 15 162.5 16 136.0 17 122.0 18 108.0 19 123.5 20 139.0 21 229.5 22 425.0 23 530.0 24 569.5 25 609.0 26 739.5 27 870.0 28 1082.0 29 1458.0 30 1622.0 31 1773.5 32 1925.0 33 1953.5 34 1982.0 35 1919.0 36 1856.0 37 1608.5 38 1587.0 39 1813.0 40 1366.0 41 919.0 42 776.0 43 633.0 44 648.0 45 663.0 46 673.0 47 1011.0 48 1339.0 49 1067.5 50 796.0 51 747.0 52 698.0 53 737.0 54 917.5 55 1059.0 56 1166.0 57 1273.0 58 1390.5 59 1508.0 60 1385.5 61 1263.0 62 1228.0 63 1103.0 64 1013.0 65 970.5 66 928.0 67 802.0 68 676.0 69 557.0 70 438.0 71 415.0 72 317.0 73 242.0 74 190.5 75 139.0 76 110.5 77 82.0 78 71.0 79 48.5 80 37.0 81 29.0 82 21.0 83 13.5 84 6.0 85 7.0 86 8.0 87 5.5 88 1.5 89 0.0 90 1.0 91 2.0 92 1.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.044239398344182515 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0031599570245844657 21 0.0 22 0.0 23 0.0 24 0.009479871073753397 25 0.03475952727042912 26 0.07899892561461164 27 0.05687922644252038 28 0.09163875371294951 29 0.18011755040131452 30 0.28755608923718634 31 0.32863553055678446 32 0.32547557353219997 33 0.3539151867534601 34 0.36339505782721354 35 0.4329141123680718 36 0.46135372558933196 37 0.48031346773683875 38 0.5308727801301902 39 0.5656323074006193 40 0.6572710611135689 41 0.6730708462364912 42 0.6983505024331669 43 0.7552297288756873 44 0.7425899007773494 45 0.7520697718511028 46 0.8089489982936232 47 0.8405485685394679 48 0.8879479239082348 49 0.8658282247361436 50 0.916387537129495 51 0.9037477090311571 52 0.9258674082032484 53 0.9258674082032484 54 1.7158566643493647 55 0.9606269354736775 56 0.9574669784490931 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 31646.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.55033811540163 #Duplication Level Percentage of deduplicated Percentage of total 1 74.85952615420263 40.83612462870505 2 12.784568151537973 13.94805030651583 3 5.2482187337079305 8.588763192820577 4 2.33447257139547 5.093850723630158 5 1.459769449110815 3.981545850976427 6 0.8399467068296357 2.7491626113884853 7 0.5966517986444998 2.2783290147254 8 0.37073509818687367 1.6178979965872464 9 0.2954295313676649 1.4504202742842698 >10 1.106412558651451 10.636415344751311 >50 0.07530556681920872 2.5690450609871704 >100 0.0173782077275097 1.425140618087594 >500 0.011585471818339802 4.825254376540479 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 941 2.973519560133982 No Hit GCGTCCGGGAGGTTACTCAGTTATTGCACCCAGTTCCTAATTAAGCTTCTGACAGA 586 1.8517348164064968 No Hit GCGTCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 203 0.6414712759906466 No Hit GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 132 0.4171143272451495 No Hit GCGTCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 116 0.36655501485179803 No Hit GCGTCCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.24015673386841938 No Hit GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAA 71 0.22435694874549705 No Hit GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 69 0.21803703469632815 Illumina Single End Adapter 2 (95% over 23bp) GCGTCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 68 0.2148770776717437 No Hit GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAA 67 0.21171712064715922 No Hit GCGTCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 65 0.20539720659799027 No Hit GCGTCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 61 0.1927573784996524 No Hit GCGTCCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 60 0.18959742147506795 No Hit GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 60 0.18959742147506795 No Hit GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 58 0.18327750742589902 No Hit GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.1674777223029767 No Hit GCGTCCGGGAGGTGACTTAATGTATCTTAATAACTACTATAGGAACAAAGGAAGTG 53 0.1674777223029767 No Hit GCGTCCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 52 0.16431776527839223 No Hit GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAA 49 0.1548378942046388 No Hit GCGTCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49 0.1548378942046388 No Hit GCGTCCTGTCCCCTGCCTGATACTTGGTGTATGCAAGAGTATTGCCCTCCGTCCGA 48 0.15167793718005435 No Hit GCGTCCGGGAGTATTCCGAGGTCTGTGTCCAGATGTTGATGCACATGCAGGATCAG 47 0.14851798015546988 No Hit GCGTCCGGGAAGAAAAGGTGGAAAAAGTGTTTTGTTTTGTTTTTTTAATTAATCAA 47 0.14851798015546988 No Hit GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 44 0.1390381090817165 Illumina Single End Adapter 2 (96% over 30bp) GCGTCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 43 0.13587815205713202 No Hit GCGTCCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.12639828098337863 No Hit GCGTCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39 0.12323832395879417 No Hit GCGTCCGGGGTATGCTTCCAGAACCTGCGGGAGGTGGGCAATGCTGTTCTCTTCTG 37 0.11691840990962522 No Hit GCGTCCGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG 36 0.11375845288504076 No Hit GCGTCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 35 0.11059849586045628 No Hit GCGTCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 35 0.11059849586045628 No Hit GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.10743853883587184 No Hit GCGTCCGGGACCCCGATGAATATCATGGCGAGTACCCAGACGACTACCCTCGGGAG 33 0.10427858181128738 No Hit GCGTCCGGGCATAGCTAGAGTGTGCTTATTTTGAGTTTTGATTCTATTTATTTTTA 33 0.10427858181128738 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 32 0.1011186247867029 No Hit GCGTCCGGGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.1011186247867029 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.006319914049168931 0.0 0.0 0.0 0.0 8 0.006319914049168931 0.0 0.0 0.0 0.0 9 0.006319914049168931 0.0 0.0 0.0 0.0 10 0.03475952727042912 0.0 0.0 0.0 0.0 11 0.06319914049168932 0.0 0.0 0.0 0.0 12 0.06635909751627378 0.0 0.0 0.0 0.0 13 0.06951905454085824 0.0 0.0 0.0 0.0 14 0.09479871073753397 0.0 0.0 0.0 0.0 15 0.10427858181128737 0.0 0.0 0.0 0.0 16 0.10427858181128737 0.0 0.0 0.0 0.0 17 0.10427858181128737 0.0 0.0 0.0 0.0 18 0.1105984958604563 0.0 0.0 0.0 0.0 19 0.13587815205713202 0.0 0.0 0.0 0.0 20 0.17379763635214562 0.0 0.0 0.0 0.0 21 0.18327750742589902 0.0 0.0 0.0 0.0 22 0.1927573784996524 0.0 0.0 0.0 0.0 23 0.23067686279466598 0.0 0.0 0.0 0.0 24 0.24331669089300387 0.0 0.0 0.0 0.0 25 0.24331669089300387 0.0 0.0 0.0 0.0 26 0.25279656196675726 0.0 0.0 0.0 0.0 27 0.2591164760159262 0.0 0.0 0.0 0.0 28 0.3507552297288757 0.0 0.0 0.0 0.0 29 0.3602351008026291 0.0 0.0 0.0 0.0 30 0.41079441319598053 0.0 0.0 0.0 0.0 31 0.4234342412943184 0.0 0.0 0.0 0.0 32 0.43923402641724074 0.0 0.0 0.0 0.0 33 0.44555394046640967 0.0 0.0 0.0 0.0 34 0.46451368261391646 0.0 0.0 0.0 0.0 35 0.597231877646464 0.0 0.0 0.0 0.0 36 0.6035517916956329 0.0 0.0 0.0 0.0 37 0.6098717057448019 0.0 0.0 0.0 0.0 38 0.6256714908677242 0.0 0.0 0.0 0.0 39 0.6383113189660621 0.0 0.0 0.0 0.0 40 0.6699108892119067 0.0 0.0 0.0 0.0 41 0.6888706313594135 0.0 0.0 0.0 0.0 42 0.6983505024331669 0.0 0.0 0.0 0.0 43 0.7173102445806737 0.0 0.0 0.0 0.0 44 0.7204702016052582 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGACAGA 50 5.456968E-12 50.71452 50 AGGCCAA 20 3.997917E-4 50.3088 47 TAGGCCA 20 3.997917E-4 50.3088 46 AGCTTCT 55 0.0 50.228436 44 TAAGCTT 55 0.0 50.228436 42 AATTAAG 55 0.0 50.228436 39 ATTGCGG 15 0.008205892 50.228436 40 ATTAAGC 55 0.0 50.228436 40 TAATTAA 55 0.0 50.228436 38 ATAGGCC 20 4.02968E-4 50.22843 45 TCCTAAT 55 0.0 50.148327 35 CCTAATT 55 0.0 50.148327 36 GTTCCTA 55 0.0 50.148327 33 TTCCTAA 60 0.0 50.148323 34 TGCAGTT 15 0.008257949 50.148323 32 CAGTTCC 55 0.0 50.06847 31 GTGCAGA 15 0.008310251 50.06847 31 GGAATGT 15 0.008310251 50.06847 31 CCAGTTC 55 0.0 49.988873 30 ACTTTCT 15 0.008362799 49.988873 30 >>END_MODULE