FastQCFastQC Report
Fri 17 Jun 2016
SRR1526786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447706
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC19290.4308631110594899No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC9670.21598995769545193No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC8680.19387723193345635No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.1791354147587926No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC7010.15657596726423142No Hit
GCCTAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTCAGAGGTCC6710.14987514127574791No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCACCCAAAAAAAAA5720.12776241551375234No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4690.10475624628662558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG150.004705955757.9357733
TAGGGGG276750.055.378874
CCTAGGG460200.055.1997872
CTAGGGG444050.055.1574863
GCCTAGG467000.054.902451
TAGGGGT26100.054.709424
TAGGGGA109250.054.2771344
AGGGGAT24400.053.8728265
AGGGGGG152900.053.047165
TAGGGGC34300.052.7372364
AGGGGGA54100.052.6805045
GCCTAGA4150.052.3574521
AGGGGAG39550.052.2810365
AGGGGAC19150.051.9371875
CTAGGGT4150.051.856683
AGGGGGC54200.051.7248085
AGGGGTG13700.050.7339255
TACGCTT404.8123184E-850.59188510
GTACGCT404.8123184E-850.5918859
AGGGGGT31050.050.2960365