FastQCFastQC Report
Fri 17 Jun 2016
SRR1526785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630589
Sequences flagged as poor quality0
Sequence length64
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC64211.0182543621915385No Hit
GCCTAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTCAGAGGTCC35020.555353804141842No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC34720.550596347224579No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC28520.45227557093447557No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC20280.32160408760698334No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC17480.2772011563791947No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT14880.2359698630962481No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC14580.23121240617898506No Hit
GCCTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC13530.21456130696856432No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12840.20361915605885927No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC11800.18712663874568064No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC11280.17888038008909132No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT10900.17285426799389142No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCACCCAAAAAAAAA10570.16762106538490204No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT9260.14684683684618666No Hit
GCCTAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG9180.14557818166824985No Hit
GCCTAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC9090.1441509445930709No Hit
GCCTAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT8680.13764908680614474No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8230.13051290143025013No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCACCCACAAAAAAA7790.12353529795159762No Hit
GCCTAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC7520.11925358672606089No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGAAGGTCC7080.11227598324740837No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCT6720.10656703494669269No Hit
GCCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG6640.10529837976875588No Hit
GCCTAGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTCAGAGGTCC6420.10180957802942964No Hit
GCCTAGGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC6370.10101666854321911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAA550.058.17741453
CTCGTAA150.00463138158.1727854
ACTACGG150.00464894258.117248
ACGACAC150.00464894258.117249
TTATCCG150.00467537658.0340337
ATTAACG150.004724129657.88216810
GACGATA150.004724129657.8821689
CCTAGGG653350.054.9227182
TAGGGGG402850.054.9125334
CTAGGGG632950.054.8630453
GCCTAGG660600.054.8110661
TAGGGGA149400.054.7506524
AGGGGAT33150.054.5032655
TAGGGGT37050.054.471094
AGGGGGC82850.054.1003345
AGGGGGG211450.053.7332085
TAGGGGC45000.053.469974
CTTACGA600.053.2995652
AGGGGGA76800.052.8576975
AGGGGAC26550.052.565885
Produced by FastQC (version 0.11.5)