FastQCFastQC Report
Fri 17 Jun 2016
SRR1526783_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526783_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567676
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC99851.758925866163093No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC47990.845376588053749No Hit
GCCTAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTC28340.499228433120301No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC19740.3477335663300897No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC15490.27286691704422944No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCACCCA14690.2587743712963028No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT14470.25489892121562296No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT13480.23745939585256376No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC11520.20293265877014355No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT10150.17879917417681918No Hit
GCCTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC9910.17457141045244118No Hit
GCCTAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC8210.14462475073809708No Hit
GCCTAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG8040.14163008476666267No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA7810.13757847786413377No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT7800.1374023210422847No Hit
GCCTAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC7430.1308845186338686No Hit
GCCTAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6660.11732044335148924No Hit
GCCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG6230.10974570001197866No Hit
GCCTAGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCC6200.10921722954643143No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC6190.10904107272458233No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC6150.10833644543718601No Hit
GCCTAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC6080.10710334768424241No Hit
GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6040.10639872039684609No Hit
GCCTAGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTC5870.10340405442541167No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC5750.10129017256322269No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC204.0822587E-450.24537745
ATAGATA150.00827015950.24091344
CGAATAC150.00832549350.15622739
ATACATC204.2330188E-449.8774623
CGATGCG150.00852578849.8554722
ACTATCG150.00853470649.8422820
CTATCGC150.00853470649.8422821
GCATAAC252.1365819E-549.7983789
CGATTTA150.00856448349.79837814
ATACACG150.00856448349.79837810
GTACGCT301.0765125E-649.7983789
CCTAGGG586850.047.4813582
CTAGGGG567400.047.479923
TAGGGGA131200.047.4553834
TAGGGGG362700.047.418024
GCCTAGG592500.047.411421
TAGGGGT32300.047.1293834
AGGGGAT29100.046.8325085
AGGGGAG48950.046.5716365
AGGGGGG188350.046.3898775
Produced by FastQC (version 0.11.5)