FastQCFastQC Report
Fri 17 Jun 2016
SRR1526782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248454
Sequences flagged as poor quality0
Sequence length64
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.2511531309618682No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6070.244310818099125No Hit
TTCATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.13282136733560337No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3300.13282136733560337No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC3120.1255765654809341No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2870.11551434068278234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTTA351.607259E-858.122656
TAGCGGA150.00464388758.1226465
GTGCGAC150.00465879258.07563449
ATATAGC150.004688709557.98182739
GCGGTAA258.676063E-657.97012338
TAGATCC150.004699965557.9467335
CGTTGAC150.00470372257.93504334
GTTATTC150.00470372257.93504334
TCGTCAA202.0264972E-457.88832517
CACGGTA202.0366162E-457.8300419
GATTAAC202.0427068E-457.7951289
ATAGGGT11850.056.4059454
TAGGGTG6200.056.2477235
TAGGGAT23250.056.1227265
TAGGGGA32400.055.611175
TAGGGTC2300.055.5955735
ATAGGGG127450.055.135574
AGGGATT8300.054.971426
CATAGGG255400.054.834193
TCATAGG259450.054.773512