FastQCFastQC Report
Fri 17 Jun 2016
SRR1526781_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526781_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331264
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC13950.4211142774343122No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC9000.2716866306027821No Hit
TTCATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.25508355873261207No Hit
TTCATAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT7360.22217928902627512No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC7000.21131182380216382No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC6940.20950057959814528No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT6720.20285935085007728No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6580.198633114374034No Hit
TTCATAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCC6490.19591624806800617No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC6230.1880675231839258No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC6110.18444503477588872No Hit
TTCATAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC4580.13825830757341576No Hit
TTCATAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG4050.12225898377125194No Hit
TTCATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.11350463678516229No Hit
TTCATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA3700.11169339258114373No Hit
TTCATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC3490.10535403786707882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT150.004652578458.0995650
CACGTGA150.004652578458.0995644
ACTTATC150.004655370558.0907639
CGTAATT150.004655370558.0907640
CGTCTAG150.00467495258.02921333
TTACGGG150.00467495258.02921333
TACACGG258.692326E-657.95903426
CGCCTAT150.00472276357.88028319
GTTATAA150.00472558657.87154815
TCGCAAC202.0304022E-457.87154416
GATCGTT202.0304022E-457.8715449
TAGGGTG7350.055.534595
TAGGGAT32700.055.2419055
ATAGGGG178100.055.150794
TAGGGGA42250.055.0885285
CATAGGG342050.055.0202263
TCATAGG345700.055.008742
ATAGGGA124400.054.942374
TAGGGGG78200.054.936245
ATAGGGT14650.054.9337124
Produced by FastQC (version 0.11.5)