FastQCFastQC Report
Fri 17 Jun 2016
SRR1526780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330402
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC13140.3976973505002996No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC8430.2551437339967676No Hit
TTCATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.24999848669196917No Hit
TTCATAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT7140.21610038680153265No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC6630.20066464488713748No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC6560.19854601364398522No Hit
TTCATAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCC6340.19188745830836373No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT6200.1876501958220592No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC5890.17826768603095622No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC5840.17675437800013316No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT5720.17312243872615782No Hit
TTCATAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC4540.13740836919873367No Hit
TTCATAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3600.10895817821926018No Hit
TTCATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA3570.10805019340076633No Hit
TTCATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.10108897645898028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTG201.9327756E-458.45110741
GTAACGT201.9327756E-458.45110740
CGTAACG258.267281E-658.45110339
CGAATTT150.00459256958.2902823
CGATGCG150.00459256958.2902822
TTGTAGT150.00466245858.06837558
CGCAACA150.00467089758.0418651
TTCGTAA258.673529E-657.98008337
ATAGGCG501.8189894E-1257.8130724
TTGCGCA258.91872E-657.70808416
TAGGGAT34950.055.3318185
ATAGGGA124950.055.0137984
TAGGGAG41600.054.9641045
TCATAGG345350.054.8165322
CATAGGG341300.054.7809753
AGGGATC10300.054.7259646
ATAGGGG177800.054.724074
TTCATAG347350.054.7175671
TAGGGGA46200.054.496955
TAGGGGG75550.054.484325
Produced by FastQC (version 0.11.5)