FastQCFastQC Report
Fri 17 Jun 2016
SRR1526779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296701
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC25870.8719215641335889No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC10030.33805076491147656No Hit
TTCATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8480.28580961978557534No Hit
TTCATAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC7060.23794999005733045No Hit
TTCATAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG6920.23323143501370064No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC6910.23289439536772713No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC6880.23188327642980644No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT5770.19447187572674174No Hit
TTCATAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT5510.18570884493142928No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA4860.16380126794314814No Hit
TTCATAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4170.1405455323709728No Hit
TTCATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT4140.13953441343305212No Hit
TTCATAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC4020.13548993768136947No Hit
TTCATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.12436762936424212No Hit
TTCATAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT3440.11594163821490322No Hit
TTCATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3280.11054900387932633No Hit
TTCATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3270.11021196423335276No Hit
TTCATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT3270.11021196423335276No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3160.10650452812764365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGA150.007986682550.6800550
CGACGTT150.00799210250.67135649
AAGGCGA150.00799210250.67135649
TAAGTCG252.019244E-550.2661247
GTCTTAT150.00826098450.24902344
TGTCGTA204.0947378E-450.2063240
TAGTCAA301.0195163E-650.1807439
ATGATAT204.156933E-450.05320432
CGTTAAG150.00838948950.05320432
ATTTGCG150.00839510950.04472431
TATTGCT150.008491090549.90101624
TTAGACC150.00855933149.80006821
TCGCAGG150.00857074549.78328312
ATAGCAT150.00857074549.78328313
TCGATGG150.00857074549.78328312
TGTTACG150.00857074549.78328311
TAGGGAG36400.048.174555
TAGGGGA43150.047.796485
TAGGGAT29300.047.521555
ATAGGGA109050.047.509894
Produced by FastQC (version 0.11.5)