FastQCFastQC Report
Fri 17 Jun 2016
SRR1526778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252446
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT37991.5048762903749713No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC8560.3390824176259477No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.27847539671850613No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC5700.22579086220419417No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4650.18419780864026367No Hit
CTCCATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT3750.14854661987118037No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3580.1418125064370202No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC2920.11566830133969246No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2830.11210318246278413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAC150.004668823458.04436548
CGAATTA150.004668823458.04436549
CTTATAT150.00467250358.0328246
ACGTACT150.00468724357.9866841
TACGTAC150.00468724357.9866840
CTACGTA150.004690933557.9751639
TCTACGT150.00469462657.9636438
TACCCGT202.0155517E-457.95212636
ACATGAT202.0175322E-457.94061731
ATTCTAC150.004702017657.94061334
CACCTAA202.0195142E-457.9291130
CCCGTAG202.0214978E-457.91761426
CATATCG258.723211E-657.9176125
TCATATC258.733485E-657.90611324
GTATTAG150.004716826657.89462327
AGATACA202.0254697E-457.89462321
ATCGGCG202.0254697E-457.89462322
GTTTAGC258.754061E-657.88313720
GTCGATC202.0314392E-457.86017616
GCATGTA202.0334321E-457.84870512
Produced by FastQC (version 0.11.5)