FastQCFastQC Report
Fri 17 Jun 2016
SRR1526776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342306
Sequences flagged as poor quality0
Sequence length64
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT84362.4644616220574576No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC18130.5296430678983133No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT17820.5205868433506862No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC16860.49254176088061563No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC15440.4510584097269695No Hit
CTCCATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT9910.28950704924833337No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC9440.275776644289028No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.2623383756054525No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC8360.24422592651019848No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT8340.24364165395873869No Hit
CTCCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG7580.2214392970032661No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC6700.19573130473903463No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC5970.1744053566107518No Hit
CTCCATGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG5340.1560007712397679No Hit
CTCCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT5000.14606813786495124No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC3790.11071964850163304No Hit
CTCCATGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCC3780.11042751222590313No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3780.11042751222590313No Hit
CTCCATGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG3770.11013537595017324No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3680.10750614946860412No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCT3500.10224769650546586No Hit
CTCCATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG3460.10107915140254627No Hit
CTCCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG3440.10049487885108646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGA201.9310546E-458.46223444
CCTAAAC201.942394E-458.3930523
GTCAATG150.00456345758.38440722
TTACGTT150.00456345758.38440722
CACTTAG150.004663150358.06663550
AACGGCA150.00466858558.04955753
CTCACGG150.0047204557.88781431
CACCTAA202.027668E-457.88781430
CATGCGA150.004758939657.769214
GGATATC150.004761697757.7607578
GGAACTA202.0498154E-457.7607548
GATATGT202.0676714E-457.6595089
CGATCCA202.0676714E-457.65950816
CATGGGT24500.055.7649734
ATGGGTA13900.055.483385
TGGGTAC12550.055.4676446
ATGGGAT18750.054.996295
TCCATGG355400.054.9571462
CCATGGG351250.054.923923
GGTACCT12050.054.8846978
Produced by FastQC (version 0.11.5)