FastQCFastQC Report
Fri 17 Jun 2016
SRR1526775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315192
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG94753.0061042158430418No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC31991.014936927333181No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC26800.8502753877001954No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC20430.6481763496535445No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT10070.3194878042589913No Hit
CTCCATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC9750.30933526231630243No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8610.27316683164547323No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC8410.2668214929312927No Hit
CTCCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC7980.25317901469580445No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC5220.1656133404401127No Hit
CTCCATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC5050.16021980253305923No Hit
CTCCATGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC4760.1510190613974974No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT4610.14626005736186198No Hit
CTCCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC4560.14467372268331682No Hit
CTCCATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA4290.13610751541917307No Hit
CTCCATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3800.12056143556943069No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC3680.11675423234092236No Hit
CTCCATGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT3500.11104342749815985No Hit
CTCCATGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC3430.10882255894819665No Hit
CTCCATGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC3360.10660169039823347No Hit
CTCCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3240.10279448716972511No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG150.0079996650.65999650
CTAATCG150.0079996650.65999649
CTAGATG204.0822304E-450.23843847
ACCGAAT204.0822304E-450.23843847
CAATTTA150.00827358250.230445
TGCATTA150.00827358250.230445
ATTAGAC204.095143E-450.20630343
ACACAAC150.00828929250.20630343
CATTAGA204.095143E-450.20630342
TTACCAT204.095143E-450.20630343
AGCGTCT204.108088E-450.17420640
CATACCG150.00842638449.99840531
TATCGGT150.0084742349.92684627
CATATCG150.00847955949.91890725
AATTTAC150.00847955949.91890726
ATATCGG150.00847955949.91890726
CGGCTAT150.00847955949.91890726
TAGGATA150.00847955949.91890725
CCTAATT150.00850623949.8792523
TAAAGCC150.00853298249.83965721
Produced by FastQC (version 0.11.5)