##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526775_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 315192 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.806321226427066 39.0 39.0 40.0 37.0 40.0 2 38.56398322292444 39.0 39.0 40.0 36.0 40.0 3 38.610599253788166 39.0 39.0 40.0 36.0 40.0 4 38.70562070103302 39.0 39.0 40.0 37.0 40.0 5 38.176010177923295 39.0 38.0 40.0 35.0 40.0 6 38.54614330312952 39.0 39.0 40.0 36.0 40.0 7 38.762236985710295 39.0 39.0 40.0 38.0 40.0 8 38.77069849488566 39.0 39.0 40.0 38.0 40.0 9 38.76773204903678 39.0 39.0 40.0 38.0 40.0 10 38.11820096956775 39.0 38.0 40.0 35.0 40.0 11 38.11516155232366 39.0 38.0 40.0 35.0 40.0 12 38.01401050788091 39.0 38.0 40.0 35.0 40.0 13 37.79162859463438 39.0 38.0 40.0 35.0 40.0 14 37.97914604431585 39.0 38.0 40.0 35.0 40.0 15 37.98041511205868 39.0 38.0 40.0 35.0 40.0 16 37.833606182898045 39.0 38.0 40.0 35.0 40.0 17 37.647849564709766 39.0 38.0 40.0 33.0 40.0 18 37.58104266605751 39.0 38.0 40.0 33.0 40.0 19 37.46489124089444 39.0 37.0 40.0 33.0 40.0 20 37.425128175842026 39.0 37.0 40.0 33.0 40.0 21 37.29938577121247 39.0 37.0 40.0 33.0 40.0 22 37.16278014670423 39.0 37.0 40.0 33.0 40.0 23 37.07716566410315 39.0 36.0 40.0 33.0 40.0 24 36.951159927916954 39.0 36.0 40.0 32.0 40.0 25 36.774375618670526 39.0 36.0 40.0 32.0 40.0 26 36.37465735170944 39.0 36.0 40.0 31.0 40.0 27 36.183437396888245 38.0 36.0 40.0 30.0 40.0 28 35.87874692251072 38.0 35.0 40.0 30.0 40.0 29 35.76124711287088 38.0 35.0 40.0 29.0 40.0 30 35.34830516002944 38.0 35.0 40.0 29.0 40.0 31 34.53538160867027 38.0 33.0 39.0 27.0 40.0 32 34.486525673240436 38.0 33.0 39.0 26.0 40.0 33 34.29532475443539 38.0 33.0 39.0 26.0 40.0 34 34.32543338663418 38.0 33.0 39.0 26.0 40.0 35 34.05305337698926 38.0 33.0 39.0 25.0 40.0 36 33.64787494606462 38.0 33.0 39.0 22.0 40.0 37 33.34531650549506 38.0 33.0 39.0 19.0 40.0 38 33.14983882839666 37.0 33.0 39.0 18.0 40.0 39 32.94984327013376 37.0 33.0 39.0 17.0 40.0 40 32.64809386025026 37.0 32.0 39.0 11.0 40.0 41 32.17760920327927 36.0 32.0 39.0 4.0 40.0 42 31.91025152922663 36.0 31.0 39.0 4.0 40.0 43 31.74536790273865 36.0 31.0 39.0 4.0 40.0 44 31.558091575928323 36.0 31.0 39.0 4.0 40.0 45 31.147227721515776 36.0 30.0 39.0 4.0 40.0 46 30.497541181248256 36.0 30.0 39.0 4.0 40.0 47 30.174157339018755 35.0 29.0 39.0 4.0 40.0 48 29.89085065610802 35.0 29.0 38.0 4.0 40.0 49 29.626827457549684 35.0 28.0 38.0 4.0 40.0 50 29.22552920124876 35.0 28.0 38.0 4.0 40.0 51 28.248305794563315 34.0 25.0 38.0 4.0 40.0 52 27.932656920226403 34.0 23.0 38.0 4.0 39.0 53 27.66298637021244 34.0 22.0 38.0 4.0 39.0 54 27.25001269067743 33.0 17.0 38.0 4.0 39.0 55 26.971046219447196 33.0 4.0 38.0 4.0 39.0 56 15.976528592096246 20.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.799892227008705 1 2 -1.106020695061197 1 3 -0.9821189982843563 1 4 -1.1656818219650376 1 5 -1.530945494713258 1 6 -1.0521335204563016 1 7 -0.7614056326855447 1 8 -0.7626174346518226 1 9 -0.9194372713701142 1 10 -0.8260906902331016 1 11 -1.0460290398126517 1 12 -1.2522178220107207 1 13 -0.7065476099941179 1 14 -1.2096477926486244 1 15 -1.0491206841921397 1 16 -0.6089603142366968 1 17 -0.7527840972364217 1 18 -0.6401566820577855 1 19 -0.6561985098956882 1 20 -0.5955396004840878 1 21 -0.4970814237176029 1 22 -0.8028728550835496 1 23 -0.8049570861035846 1 24 -0.7366568017207058 1 25 -1.1445989656672353 1 26 -0.5792552982545729 1 27 -1.0256825147347683 1 28 -0.4183272879875375 1 29 -0.24425667761463643 1 30 -0.00806317893466968 1 31 -0.4869397264027171 1 32 -0.38297074401083364 1 33 -0.624152713091199 1 34 -0.6194020249709098 1 35 -0.9072189473908523 1 36 0.8514810129392743 1 37 0.9661561434547394 1 38 0.8858476477500759 1 39 0.39853488005925186 1 40 0.6923971267453481 1 41 0.8434662463624463 1 42 1.029100106396136 1 43 1.2432096206871037 1 44 0.8257798801893372 1 45 0.9918553548367086 1 46 0.8946793199649186 1 47 1.1273927670693773 1 48 0.3391003655610376 1 49 0.9174666134880027 1 50 0.6058010072128397 1 51 0.998848441914653 1 52 1.1313430479353457 1 53 0.8441261336806569 1 54 0.7885775332071994 1 55 0.4020138725727378 1 56 0.9212045504992563 2 1 -0.33285117874022063 2 2 -0.3749798412766623 2 3 -0.42196875943376 2 4 -0.28896033513525055 2 5 -0.4840383972646478 2 6 -0.35695064476801264 2 7 -0.1688130400929495 2 8 -0.1604100418307084 2 9 -0.15067868672704776 2 10 -0.693051513872625 2 11 -0.19567228331871434 2 12 -0.47595945008447416 2 13 -0.296575445509319 2 14 -0.5272157708664196 2 15 -0.405500365481565 2 16 -0.42610893643947634 2 17 -0.681031675303494 2 18 -0.587670326322332 2 19 -0.5300513584777775 2 20 -0.5716180747575024 2 21 -0.7956264406741695 2 22 -1.132851340296689 2 23 -0.6858721330836133 2 24 -0.8630835238152983 2 25 -1.2823908435313172 2 26 -0.8874515811800947 2 27 -0.7232842845387069 2 28 -0.48339432417370887 2 29 -0.825727219373988 2 30 -0.35439047111033517 2 31 -0.9660630900026135 2 32 -0.20868881521627003 2 33 -0.9476524517533989 2 34 -0.3858875784600784 2 35 -0.39769531151343074 2 36 -0.3461146792412535 2 37 0.0662181352134823 2 38 0.14073101815691302 2 39 -0.12676626618052467 2 40 -0.23309121029533486 2 41 0.6548775920698233 2 42 0.08085714732285254 2 43 0.5060789882496124 2 44 0.45320941014594496 2 45 0.6411278633149919 2 46 0.4658422124274466 2 47 0.6247976218749542 2 48 0.3055275738655041 2 49 0.6855080385691394 2 50 0.8127562394388299 2 51 1.080519545246407 2 52 0.5849525484762559 2 53 0.6040478538911884 2 54 0.9557851083570128 2 55 0.9143453704638027 2 56 0.6899996008827554 3 1 -0.17952037353228434 3 2 -0.23948381158268006 3 3 -0.34426337797410866 3 4 -0.17418344220136106 3 5 -0.60417031944435 3 6 -0.38873607587084535 3 7 -0.0992895917299137 3 8 -0.02922331678733059 3 9 -0.11856000273123612 3 10 -0.189196122645221 3 11 -0.3878557792564763 3 12 -0.07938850743844483 3 13 0.03136838414099685 3 14 -0.015293361333085898 3 15 0.0427707414926104 3 16 0.17686046175176529 3 17 -0.22087837897272067 3 18 -0.007833275495521264 3 19 -0.3122648471759035 3 20 0.010207001948117522 3 21 -0.12347833849193535 3 22 0.2291991992282547 3 23 0.3264237495194422 3 24 0.45570159701760105 3 25 0.5470756070955431 3 26 0.8636544736802279 3 27 0.6249994630624442 3 28 1.0620142553645664 3 29 0.7408716475661734 3 30 0.7411267029051345 3 31 0.6596824197019373 3 32 0.6382217006362225 3 33 1.0848454510516774 3 34 0.8518711234624519 3 35 0.761254826958897 3 36 1.5287890796215464 3 37 1.5565135811403294 3 38 1.738954435492957 3 39 1.1855261689788108 3 40 1.3569157995069006 3 41 1.8680100543490425 3 42 1.7768730515298827 3 43 1.7314613672156085 3 44 2.1222892874169474 3 45 1.6548831637597132 3 46 1.5537520272997227 3 47 2.082284895061889 3 48 2.253172395230461 3 49 2.163496110413867 3 50 2.2739667518438793 3 51 2.65006023082805 3 52 2.437068704346675 3 53 1.9613868869981381 3 54 1.8613711163321014 3 55 2.2386349097704077 3 56 0.9842274309467616 4 1 -0.3672336841124846 4 2 -0.46473978427892604 4 3 -0.542215596404148 4 4 -0.740736343225251 4 5 -0.8737948928231916 4 6 -0.5798623557343277 4 7 -0.2631121173271822 4 8 -0.2920219515996152 4 9 -0.30627331331143637 4 10 -0.8240084093346383 4 11 -0.2752277929083533 4 12 -0.21249430948623171 4 13 -0.13245414145627166 4 14 -0.43850155245937117 4 15 -0.33606884895107214 4 16 -0.27365148364072667 4 17 -0.33350636532084366 4 18 -0.37643395808937896 4 19 -0.015336519530855242 4 20 0.12624795757951546 4 21 -0.37945103473767006 4 22 0.01759510366511563 4 23 -0.325453383586364 4 24 -0.319042430286153 4 25 0.16482304736857856 4 26 0.1503438934754442 4 27 0.020903899922515734 4 28 0.07907910178094824 4 29 0.3654450264057658 4 30 0.48856697594267473 4 31 0.5095117712377757 4 32 0.576788253537245 4 33 0.5012277036113986 4 34 0.606137335066542 4 35 0.764667796242172 4 36 0.4571167246727441 4 37 0.22886852994579243 4 38 0.6570431726943227 4 39 0.33910978672624736 4 40 0.6026495879028033 4 41 0.922617562881463 4 42 0.5345522324172549 4 43 0.18197331260810046 4 44 0.6137568642087601 4 45 0.8016408429405999 4 46 0.35921277815296193 4 47 0.7887695367001122 4 48 0.30095396519633155 4 49 0.4877281923251289 4 50 0.9429087313660638 4 51 1.8548383878246355 4 52 1.0036216053705687 4 53 1.1968817675442125 4 54 0.7282653142843181 4 55 0.8427304729103398 4 56 0.14463698726075513 5 1 -0.3018771442074666 5 2 -0.34721704127186825 5 3 -0.32919044245615225 5 4 -0.34761329414407527 5 5 -0.2804605068540127 5 6 -0.41834753178767414 5 7 -0.31300705617309177 5 8 -0.11147466193447997 5 9 -0.1555075207373804 5 10 -0.07910660811276671 5 11 -0.24634508488119167 5 12 -0.22762797399499135 5 13 0.04274899701796642 5 14 -0.29243618377366687 5 15 -0.27249774137573723 5 16 -0.24575258526461852 5 17 0.14565751793374204 5 18 -0.46820760899484526 5 19 0.25730021179194296 5 20 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0.06194290409035119 7 29 -0.24721268963994447 7 30 0.3214503998524876 7 31 0.4864058502712041 7 32 0.3796803585187547 7 33 0.33041275314107565 7 34 0.255778794913617 7 35 0.7536321324969464 7 36 0.6203208725601499 7 37 0.09110111131597165 7 38 0.17278207459821004 7 39 0.4577431719874525 7 40 0.5462820812730769 7 41 0.31557281374065127 7 42 0.2440336275725059 7 43 -0.15039281456365572 7 44 -0.27117731424078073 7 45 0.08564269807373037 7 46 0.4776677852286326 7 47 0.32726949507853575 7 48 0.4506489384990644 7 49 0.5274113926676165 7 50 0.4445914322496307 7 51 0.6735110162866604 7 52 1.3277228766124374 7 53 0.9969079296780912 7 54 1.1851988743561463 7 55 1.4388822608543528 7 56 0.6012256828405445 8 1 -0.5265628760490415 8 2 -0.7550999875838542 8 3 -0.43762895854719375 8 4 -0.39780659146352093 8 5 -0.24552617673803923 8 6 -0.26607788842763114 8 7 -0.4088876470740317 8 8 -0.43170815485544267 8 9 -0.454811138341519 8 10 -0.0716819428627602 8 11 -0.15060530659705762 8 12 -0.2226227255247295 8 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1.6895688404093931 120 47 1.7752023786626907 120 48 1.679551501080919 120 49 1.866144187290395 120 50 1.7152564185347536 120 51 2.223366454050762 120 52 2.121344840187696 120 53 2.265843111431124 120 54 2.1056525773734194 120 55 1.8374415807940565 120 56 1.7938394174885293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 214.0 5 15.0 6 44.0 7 89.0 8 168.0 9 192.0 10 228.0 11 346.0 12 402.0 13 516.0 14 722.0 15 958.0 16 1149.0 17 1482.0 18 1953.0 19 2255.0 20 2274.0 21 2905.0 22 3081.0 23 3257.0 24 4255.0 25 5684.0 26 5650.0 27 5496.0 28 6235.0 29 6427.0 30 7247.0 31 8734.0 32 10541.0 33 13289.0 34 17447.0 35 23455.0 36 34117.0 37 48469.0 38 58791.0 39 37105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.45208000203051 0.45718165435670954 0.051079976649153536 0.03965836696362852 8 99.58137748705889 0.262789568396698 0.08600962926993376 0.06982331527448497 9 98.83182314271936 0.6602324932104876 0.18845655981116272 0.3194878042589913 10 62.16655245056981 23.375593289169778 7.447206781898018 7.010647478362395 11 41.07940556866926 24.391482017310086 15.192961750298231 19.336150663722428 12 37.451140891900806 13.510495190233254 23.229333231807914 25.809030686058023 13 28.088910886063097 12.903563542221882 23.85117642579761 35.15634914591741 14 23.072603365567655 16.427764664077767 35.429198710627176 25.070433259727405 15 29.82912002842712 16.890657123277244 27.315414096804485 25.96480875149115 16 37.36325795070941 16.8259346683926 21.68646412345491 24.12434325744308 17 33.46595091245971 20.231477956293308 24.378156806010303 21.924414325236683 18 32.59283230538846 17.893537907053478 28.357318713672935 21.156311073885124 19 37.284893017589276 21.3593619127389 19.947841315769434 21.40790375390238 20 33.373203464576925 26.322218344490622 19.61071099971446 20.693867191217997 21 32.177529886545344 20.937079621310186 26.562539658366962 20.322850833777505 22 28.575598365440747 19.324411787101194 25.718609609380948 26.381380238077107 23 29.202293212686907 19.798471403054023 24.784971652109355 26.21426373214971 24 31.487351310875685 20.512674072170345 24.024101202204516 23.975873414749454 25 33.58341346687867 18.49101393530287 19.819802665194114 28.105769932624348 26 25.839625097630826 18.982289927038817 23.552365047212046 31.62571992811831 27 24.45499977774532 16.87167014865663 24.66360166881513 34.00972840478292 28 25.52432559483917 25.25116490329794 21.4739242709627 27.750585230900192 29 33.60476240421319 21.805543636831718 20.24593889349746 24.343755065457632 30 30.71202033344043 18.898774967473493 27.186115237674123 23.203089461411952 31 31.77365456223545 21.219248273447693 24.750962731930876 22.256134432385984 32 38.68282110770582 17.67536447741529 22.044319689895836 21.59749472498305 33 34.94930523501043 18.13806166138762 22.991070718003407 23.92156238559855 34 28.233140164299815 22.708081258358277 29.406164427179522 19.65261415016239 35 26.15758070013921 25.77371148963553 29.986715895370953 18.081991914854306 36 27.773829744517986 30.188114047527236 21.91211571738597 20.1259404905688 37 29.277473227944927 25.013704742478325 24.670767465578784 21.03805456399796 38 33.63058421435221 24.206126535140857 21.24775228597299 20.915536964533942 39 31.050430755392817 21.21593906589393 20.832230263556596 26.90139991515666 40 29.34551873617411 19.82564616577979 24.81493658844898 26.013898509597123 41 25.43908661045251 21.687574032508852 25.59393627856338 27.279403078475255 42 26.957529771314153 20.384687852281907 24.680890472860124 27.976891903543816 43 24.023078102637495 18.03408045389491 29.666734819055407 28.276106624412183 44 26.158569114833067 17.687685481268986 24.325878410539328 31.82786699335861 45 26.660786926525613 19.55397058212623 26.62339944845481 27.16184304289334 46 25.278027292866074 21.114730411822432 24.073855045408195 29.533387249903303 47 24.19938726822685 23.288604340289993 26.837395826004645 25.674612565478512 48 25.856486517616045 22.752082826757444 27.333661807804045 24.05776884782247 49 28.68103996955188 21.858998346462485 24.455404061241655 25.004557622743977 50 28.778035229658457 18.849531466024885 24.704285933739566 27.66814737057709 51 24.31785604586874 23.504530562096857 26.71527848878878 25.46233490324562 52 23.7816040453178 20.195865070729052 29.531139985918198 26.49139089803495 53 25.289074226388404 18.006611982858768 31.587099912629657 25.117213878123167 54 24.46383227234449 21.429285873307286 27.623890478848516 26.482991375499708 55 25.10319165628612 21.454578059004174 24.9091369046666 28.533093380043102 56 26.986073315191287 19.518168720986072 22.599327677285096 30.89643028653754 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.5 5 3.0 6 8.0 7 13.0 8 17.0 9 21.0 10 123.5 11 226.0 12 232.5 13 271.0 14 303.0 15 301.0 16 299.0 17 387.0 18 475.0 19 604.0 20 733.0 21 701.5 22 771.5 23 873.0 24 1070.0 25 1267.0 26 1645.5 27 2024.0 28 2363.5 29 2849.0 30 2995.0 31 3263.5 32 3532.0 33 3252.5 34 2973.0 35 3429.5 36 3886.0 37 3575.0 38 3398.5 39 3533.0 40 3984.5 41 4436.0 42 4290.0 43 4144.0 44 5387.0 45 6630.0 46 12206.5 47 12814.5 48 7846.0 49 8309.5 50 8773.0 51 10323.0 52 11873.0 53 11997.5 54 13805.5 55 15489.0 56 17657.0 57 19825.0 58 22378.5 59 24932.0 60 29056.5 61 33181.0 62 33330.0 63 27934.0 64 22389.0 65 19737.5 66 17086.0 67 15128.5 68 13171.0 69 11562.0 70 9953.0 71 8685.0 72 6539.0 73 5661.0 74 4626.0 75 3591.0 76 2900.0 77 2209.0 78 1794.0 79 1106.5 80 834.0 81 676.0 82 518.0 83 391.0 84 264.0 85 194.0 86 124.0 87 79.5 88 22.0 89 9.0 90 8.0 91 7.0 92 3.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.03521662986370212 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 6.345338714180564E-4 21 0.0 22 0.0 23 3.172669357090282E-4 24 0.006662605649889592 25 0.029505825020939618 26 0.07297139521307647 27 0.0755095306987487 28 0.11389882991954112 29 0.17798675093276478 30 0.2642833574456205 31 0.31155613086626566 32 0.30933526231630243 33 0.33535115104444274 34 0.3591461712226199 35 0.4070534785146831 36 0.44195284144267616 37 0.4543262519353283 38 0.4885910809919033 39 0.5307875834412041 40 0.6094697834970431 41 0.6297748673824208 42 0.6519835528820528 43 0.687834716617173 44 0.6837102464529556 45 0.7148024061524404 46 0.7497017690804335 47 0.7912637376583163 48 0.8363156425289983 49 0.8023680804081322 50 0.8655042006142288 51 0.8401228457575065 52 0.8667732683570649 53 0.8658214675499378 54 1.669141348765197 55 0.9229295159775629 56 0.9016726312850579 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 315192.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.03001498222466 #Duplication Level Percentage of deduplicated Percentage of total 1 64.29881761160982 21.23790909050803 2 13.715109737345998 9.060205602147873 3 6.843418686374248 6.781146652217326 4 4.019584458015761 5.310677394823119 5 2.5039670823678977 4.1353035122804505 6 1.7674796572048999 3.5027927734953086 7 1.198563032386606 2.771198844180915 8 0.907638174515736 2.398344200215504 9 0.6804793378517552 2.0228618451904006 >10 3.668411094301178 21.789449087699758 >50 0.2550551979263386 5.831975841795919 >100 0.13051652706386857 7.620202719367245 >500 0.00597785620139856 1.4815232614696552 >1k 0.00398523746759904 2.938365437952588 >5k 9.9630936689976E-4 3.118043736655927 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 9475 3.0061042158430418 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 3199 1.014936927333181 No Hit CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 2680 0.8502753877001954 No Hit CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 2043 0.6481763496535445 No Hit CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 1007 0.3194878042589913 No Hit CTCCATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 975 0.30933526231630243 No Hit CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 861 0.27316683164547323 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 841 0.2668214929312927 No Hit CTCCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 798 0.25317901469580445 No Hit CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 522 0.1656133404401127 No Hit CTCCATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 505 0.16021980253305923 No Hit CTCCATGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC 476 0.1510190613974974 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 461 0.14626005736186198 No Hit CTCCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC 456 0.14467372268331682 No Hit CTCCATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA 429 0.13610751541917307 No Hit CTCCATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 380 0.12056143556943069 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC 368 0.11675423234092236 No Hit CTCCATGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT 350 0.11104342749815985 No Hit CTCCATGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 343 0.10882255894819665 No Hit CTCCATGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC 336 0.10660169039823347 No Hit CTCCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 324 0.10279448716972511 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0012690677428361125 0.0 0.0 0.0 0.0 9 0.002855402421381253 0.0 0.0 0.0 0.0 10 0.006979872585598619 0.0 0.0 0.0 0.0 11 0.016180613721160437 0.0 0.0 0.0 0.0 12 0.016815147592578492 0.0 0.0 0.0 0.0 13 0.018401482271123634 0.0 0.0 0.0 0.0 14 0.023795020178177112 0.0 0.0 0.0 0.0 15 0.025064087921013223 0.0 0.0 0.0 0.0 16 0.026333155663849337 0.0 0.0 0.0 0.0 17 0.029505825020939618 0.0 0.0 0.0 0.0 18 0.03172669357090281 0.0 0.0 0.0 0.0 19 0.03553389679941115 0.0 0.0 0.0 0.0 20 0.042196502449300746 0.0 0.0 0.0 0.0 21 0.045051904870681996 0.0 0.0 0.0 0.0 22 0.04917637503489936 0.0 0.0 0.0 0.0 23 0.05361811213482576 0.0 0.0 0.0 0.0 24 0.057425315363334097 0.0 0.0 0.0 0.0 25 0.060597984720424374 0.0 0.0 0.0 0.0 26 0.06218431939896952 0.0 0.0 0.0 0.0 27 0.06599152262747786 0.0 0.0 0.0 0.0 28 0.08090306860580218 0.0 0.0 0.0 0.0 29 0.08375847102718344 0.0 0.0 0.0 0.0 30 0.09169014441990914 0.0 0.0 0.0 0.0 31 0.09391101296987234 0.0 0.0 0.0 0.0 32 0.09486281377699941 0.0 0.0 0.0 0.0 33 0.09613188151983552 0.0 0.0 0.0 0.0 34 0.09930455087692582 0.0 0.0 0.0 0.0 35 0.11262976217670499 0.0 0.0 0.0 0.0 36 0.1161196984695043 0.0 0.0 0.0 0.0 37 0.11865783395517653 0.0 0.0 0.0 0.0 38 0.1218305033122668 0.0 0.0 0.0 0.0 39 0.12373410492652098 0.0 0.0 0.0 0.0 40 0.12658950734790222 0.0 0.0 0.0 0.0 41 0.13166577831924667 0.0 0.0 0.0 0.0 42 0.13420391380491892 0.0 0.0 0.0 0.0 43 0.13705931622630016 0.0 0.0 0.0 0.0 44 0.14023198558339045 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTACGG 15 0.00799966 50.659996 50 CTAATCG 15 0.00799966 50.659996 49 CTAGATG 20 4.0822304E-4 50.238438 47 ACCGAAT 20 4.0822304E-4 50.238438 47 CAATTTA 15 0.008273582 50.2304 45 TGCATTA 15 0.008273582 50.2304 45 ATTAGAC 20 4.095143E-4 50.206303 43 ACACAAC 15 0.008289292 50.206303 43 CATTAGA 20 4.095143E-4 50.206303 42 TTACCAT 20 4.095143E-4 50.206303 43 AGCGTCT 20 4.108088E-4 50.174206 40 CATACCG 15 0.008426384 49.998405 31 TATCGGT 15 0.00847423 49.926846 27 CATATCG 15 0.008479559 49.918907 25 AATTTAC 15 0.008479559 49.918907 26 ATATCGG 15 0.008479559 49.918907 26 CGGCTAT 15 0.008479559 49.918907 26 TAGGATA 15 0.008479559 49.918907 25 CCTAATT 15 0.008506239 49.87925 23 TAAAGCC 15 0.008532982 49.839657 21 >>END_MODULE