FastQCFastQC Report
Fri 17 Jun 2016
SRR1526774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753349
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT521526.922687891004037No Hit
TTAACTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT29080.38600967148028337No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT26500.3517625960876035No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC19180.2545964752060466No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT16360.21716362535823372No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC13110.17402292961164081No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC13100.1738901890093436No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT11470.15225347083489857No Hit
TTAACTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG9930.1318114180811284No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAC8320.11044018111127778No Hit
TTAACTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG8100.10751988786073918No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAG7940.10539603822398383No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC7830.10393589159871455No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT7780.10327218858722849No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAGCCATGCAT7720.10247574497344525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTA150.004667664458.05933448
GATATAC150.00474950557.8049939
TGGGTAC116750.057.43016
ACTGGGT149000.057.224564
AACTAGG3100.057.164813
CTGGGTA120500.057.16175
AACTGGG746550.056.957883
TAACTGG756100.056.9531172
GGTACCT115500.056.9353878
CATGCAT67700.056.89145758
GGGTACC115800.056.872677
TTAACTG762750.056.7813071
ACTGGGG402550.056.6297264
GTACCTG116650.056.5165829
ACTGGGA130450.056.4985164
TACCTGG116850.056.27144610
GTTGATC114800.056.1386216
CTGGTTG116400.056.11280413
ACCTGGT116800.056.0940711
TAGCATA101000.056.09012230
Produced by FastQC (version 0.11.5)