FastQCFastQC Report
Fri 17 Jun 2016
SRR1526772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080133
Sequences flagged as poor quality0
Sequence length64
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT12003411.112890727345615No Hit
TTAACTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT82000.7591657694006202No Hit
TTAACTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG70020.6482535021150173No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC42040.3892113286049033No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC36510.3380139297660566No Hit
TTAACTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG28320.26218993401738494No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC24810.22969393583938272No Hit
TTAACTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG24710.22876812392547954No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT23680.21923226121227665No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC21340.19756826242694187No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC20450.18932853639320343No Hit
TTAACTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT20270.1876620749481777No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT19720.1825701094217101No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT17980.1664609821197945No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC17040.15775835012910447No Hit
TTAACTGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATC16210.15007411124370795No Hit
TTAACTGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT15060.1394272742338212No Hit
TTAACTGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG13760.12739171935307966No Hit
TTAACTGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT13680.12665106982195712No Hit
TTAACTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA13260.1227626597835637No Hit
TTAACTGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTC13090.12118877952992826No Hit
TTAACTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC13020.12054071119019602No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC12680.11739295068292516No Hit
TTAACTGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGA12480.11554132685511877No Hit
TTAACTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG12410.11489325851538654No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC11620.10757934439555128No Hit
TTAACTGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGG11540.10683869486442873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG150.004549532758.43819839
CATATAG150.004666349458.0651850
GTCGTAA201.9991813E-458.0651856
TAATCGC303.7323844E-758.0651850
ATCATAC150.0047871457.6915610
TGGGTAC160200.057.053676
CTGGGTA166200.057.0108225
TAACTGG1086200.056.8221972
ACTGGGT207450.056.804234
AACTGGG1072150.056.777133
TTAACTG1093850.056.7026141
CATGCAT141200.056.64436358
ACTGGGG582350.056.607294
GGTACCT158100.056.5790378
GGGTACC159500.056.3800967
ACTGGGA180700.056.3060154
GCCAGTA151900.056.27892725
GTACCTG158850.056.2751469
CCAGTAG151800.056.17276426
ATCCTGC155550.056.15196220
Produced by FastQC (version 0.11.5)