FastQCFastQC Report
Fri 17 Jun 2016
SRR1526771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986737
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG12961713.135921729903712No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC74990.7599796095616157No Hit
TTAACTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC69940.7088008253465716No Hit
TTAACTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC59740.6054298156448983No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC55680.564284100018546No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC27190.2755546817439703No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT26910.2727170461835322No Hit
TTAACTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT26050.2640014512479009No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA25830.26177188045041383Illumina Single End Adapter 1 (95% over 21bp)
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC24950.2528535972604655No Hit
TTAACTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC21620.21910600291668397No Hit
TTAACTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC17910.18150733174087927No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT16560.16782587457448137No Hit
TTAACTGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC15380.15586726756977798No Hit
TTAACTGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT14420.14613823136256165No Hit
TTAACTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA13990.141780433894746No Hit
TTAACTGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT12830.13002451514435964No Hit
TTAACTGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAA12310.12475462053211747No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC12230.12394386751484944No Hit
TTAACTGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC11970.12130892020872837No Hit
TTAACTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT11600.11755918750386374No Hit
TTAACTGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT11460.1161403697236447No Hit
TTAACTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA10820.1096543455855005No Hit
TTAACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT10800.10945165733118349No Hit
TTAACTGGGCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA10690.10833687193243995No Hit
TTAACTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG10040.10174950366713723No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT9980.10114143890418623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAA309.573141E-750.6585449
ACGATTA309.579944E-750.6533248
TCGTACC204.085253E-450.2416346
ATAATCG150.00839417950.05484833
ATGTTCG150.00843310749.99628429
ATTCGTT204.2644973E-449.80627410
ACGTCGT204.2644973E-449.80627415
GATTAAG148850.049.35080350
TGGGTAC168950.049.315116
CTGGGTA173900.049.1861465
GTAGCGT4900.048.97595629
TAACTGG996650.048.9691432
ACTGGGT208200.048.966964
TTAACTG1000850.048.9478451
AACTGGG986400.048.9224663
GGTACCT166750.048.905198
GGGTACC167600.048.8057757
GTACCTG167200.048.7785729
ACTGGGG527300.048.6496124
ACTGGGA163800.048.6150634
Produced by FastQC (version 0.11.5)