##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526771_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	986737
Sequences flagged as poor quality	0
Sequence length	56
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.65356320883883	39.0	38.0	40.0	36.0	40.0
2	38.59291077561701	39.0	38.0	40.0	37.0	40.0
3	38.376181292482194	39.0	38.0	40.0	35.0	40.0
4	38.42763269239929	39.0	38.0	40.0	36.0	40.0
5	38.38167921138054	39.0	38.0	40.0	35.0	40.0
6	38.591137253391736	39.0	38.0	40.0	36.0	40.0
7	38.7942764890746	39.0	38.0	40.0	38.0	40.0
8	38.77790333189087	39.0	38.0	40.0	38.0	40.0
9	38.7831235678808	39.0	38.0	40.0	38.0	40.0
10	38.17153912339357	39.0	38.0	40.0	35.0	40.0
11	38.172054965000804	39.0	38.0	40.0	35.0	40.0
12	38.05626828628095	39.0	38.0	40.0	35.0	40.0
13	37.894833172365075	39.0	38.0	40.0	35.0	40.0
14	38.06208138541476	39.0	38.0	40.0	35.0	40.0
15	37.98176109743528	39.0	38.0	40.0	35.0	40.0
16	37.881456761021425	39.0	38.0	40.0	35.0	40.0
17	37.68764523880223	39.0	38.0	40.0	33.0	40.0
18	37.68458059239696	39.0	38.0	40.0	33.0	40.0
19	37.49999442607301	39.0	37.0	40.0	33.0	40.0
20	37.43828294672238	39.0	37.0	40.0	33.0	40.0
21	37.35336062192864	39.0	37.0	40.0	33.0	40.0
22	37.2984260243611	39.0	37.0	40.0	33.0	40.0
23	37.25876804052144	39.0	37.0	40.0	33.0	40.0
24	37.04962416530443	39.0	36.0	40.0	33.0	40.0
25	36.81861428121171	39.0	36.0	40.0	32.0	40.0
26	36.53924196619768	39.0	36.0	40.0	31.0	40.0
27	36.410013002451514	39.0	36.0	40.0	31.0	40.0
28	35.88449607139491	38.0	35.0	40.0	30.0	40.0
29	35.893356588432376	38.0	35.0	40.0	30.0	40.0
30	35.382207214283035	38.0	35.0	39.0	29.0	40.0
31	34.78028694576164	38.0	34.0	39.0	27.0	40.0
32	34.73237752308873	38.0	34.0	39.0	27.0	40.0
33	34.48879590002199	38.0	33.0	39.0	26.0	40.0
34	34.56314600547056	38.0	33.0	40.0	27.0	40.0
35	34.28483476346788	38.0	33.0	39.0	26.0	40.0
36	34.07413322901645	38.0	33.0	39.0	24.0	40.0
37	33.73066480733975	38.0	33.0	39.0	23.0	40.0
38	33.74408479665808	38.0	33.0	39.0	23.0	40.0
39	33.58054071145604	38.0	33.0	39.0	22.0	40.0
40	33.164658870600775	37.0	33.0	39.0	18.0	40.0
41	32.82382235590639	37.0	32.0	39.0	12.0	40.0
42	32.5538892328959	37.0	32.0	39.0	10.0	40.0
43	32.25200332003361	36.0	32.0	39.0	4.0	40.0
44	32.27181407001055	36.0	32.0	39.0	4.0	40.0
45	31.779186348540694	36.0	31.0	39.0	4.0	40.0
46	31.264303456746834	36.0	31.0	39.0	4.0	40.0
47	30.787918158536673	36.0	30.0	39.0	4.0	40.0
48	30.619972697892145	35.0	30.0	39.0	4.0	40.0
49	30.319169140307903	35.0	29.0	39.0	4.0	40.0
50	30.034083043404678	35.0	29.0	39.0	4.0	40.0
51	28.882054691371664	35.0	27.0	38.0	4.0	39.0
52	28.674549550690813	35.0	27.0	38.0	4.0	40.0
53	28.48313684396146	34.0	26.0	38.0	4.0	40.0
54	28.02379864138063	34.0	23.0	38.0	4.0	40.0
55	27.748554072665765	34.0	22.0	38.0	4.0	40.0
56	16.539032183854463	20.0	4.0	23.0	4.0	26.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.7247405084709868
1	2	-0.8198222585181085
1	3	-0.8887385277051933
1	4	-1.0181575856093588
1	5	-1.2144551276998996
1	6	-0.8813543534657882
1	7	-0.7060534936991374
1	8	-0.7645051686806426
1	9	-0.8468987833867629
1	10	-0.5460861989316115
1	11	-0.8599921964938062
1	12	-0.9845601596478915
1	13	-0.702677920242067
1	14	-0.9891542432509368
1	15	-1.0231464688530494
1	16	-0.5928116706989002
1	17	-0.508796683732875
1	18	-0.7100719226910428
1	19	-0.5875656822533415
1	20	-0.5559921599543216
1	21	-0.375907608031973
1	22	-0.7507450752719151
1	23	-0.49874092665226755
1	24	-0.5488351160928957
1	25	-0.8214178627116837
1	26	-0.6226215655070177
1	27	-0.7468157381781992
1	28	-0.20726113095531673
1	29	0.0026861459585347802
1	30	-0.11750906251478455
1	31	-0.00896702513510661
1	32	0.07578337313265848
1	33	0.16958453409332463
1	34	-0.5192006306692605
1	35	-0.3578166706369714
1	36	0.718935265327822
1	37	0.902512022017163
1	38	0.9949097514262988
1	39	0.8127740862117818
1	40	0.952197656471391
1	41	1.22226898037907
1	42	0.8290889786961344
1	43	1.5700646084210632
1	44	1.2788714519942062
1	45	1.2993683900680324
1	46	1.6338929951477397
1	47	1.8781757418789553
1	48	1.3979276406228713
1	49	1.971484521945591
1	50	1.744954796910978
1	51	2.3501218819298515
1	52	2.339933458687465
1	53	2.168402860208136
1	54	2.3750348340677867
1	55	2.105966745045464
1	56	2.0627614473337914
2	1	-0.2328126596674096
2	2	-0.132727312171113
2	3	-0.31690960348048236
2	4	-0.22367989085897477
2	5	-0.1810875603237676
2	6	-0.27973472909587116
2	7	-0.06697828504854897
2	8	-0.04195555883068636
2	9	-0.09949289749236812
2	10	-0.26365298482718913
2	11	-0.4163071969069776
2	12	-0.4646548344835111
2	13	-0.3111502555940291
2	14	-0.2610693428247828
2	15	-0.298987858991282
2	16	-0.30245268544314996
2	17	-0.2734954831804117
2	18	-0.16155402064762825
2	19	-0.1708889814706822
2	20	-0.20915054552408208
2	21	-0.19012772112235865
2	22	-0.24331864895183486
2	23	-0.26654203866841897
2	24	-0.3149398722638779
2	25	-0.19910316922441496
2	26	0.018551363628510842
2	27	-0.08790367244883868
2	28	-0.10073423362203471
2	29	0.0176263544320463
2	30	-0.04020623289407155
2	31	0.15681073690288372
2	32	0.22259193400432764
2	33	-0.03675230527818485
2	34	0.09025386756592013
2	35	0.422967406991674
2	36	0.23841561616773532
2	37	0.6385498447320401
2	38	0.8615221158181754
2	39	0.4183412498623156
2	40	0.607101978224172
2	41	0.6354113113632422
2	42	0.36923606703177114
2	43	0.8674356700305381
2	44	0.7418491054276473
2	45	0.8215756594899517
2	46	0.9436355315361666
2	47	0.8814645709566413
2	48	0.5013203523132077
2	49	0.9538138534373672
2	50	0.5726702057716935
2	51	0.9749073289202066
2	52	1.2073733425278164
2	53	0.9100019441523699
2	54	0.9066340360604457
2	55	0.9729202693811452
2	56	0.7605964392474753
3	1	-0.2313058727808368
3	2	-0.2462958934724213
3	3	-0.3562510476191818
3	4	-0.37825245833845145
3	5	-0.6259727341638239
3	6	-0.3515101647776433
3	7	-0.16752573556744466
3	8	-0.13160476361409934
3	9	-0.18743687178699986
3	10	-0.3842524691045668
3	11	-0.6114799409089571
3	12	-0.2848487784924174
3	13	-0.07192072441704056
3	14	-0.47094768074423854
3	15	-0.3060072245486296
3	16	-0.34811432756396243
3	17	-0.6518559006042182
3	18	-0.37174848384037773
3	19	-0.4906104681824601
3	20	-0.36463572282536205
3	21	-0.5695322749415368
3	22	-0.20086902420638353
3	23	-0.4327800902291372
3	24	-0.3626009490511706
3	25	-0.16809981760427206
3	26	-0.04246196785883427
3	27	-0.22761258337419576
3	28	0.020304826309086366
3	29	0.06403908251239443
3	30	0.24185711907219343
3	31	-0.14511082018714916
3	32	0.24003535738721382
3	33	0.1717634634888654
3	34	0.14194727220049685
3	35	0.20196815115518518
3	36	0.5901176738663665
3	37	0.6078314673546146
3	38	0.6267564119491027
3	39	0.4696319985831039
3	40	0.09221351621977902
3	41	0.7899158080721875
3	42	0.7584416580648892
3	43	0.555069287761242
3	44	0.9345113071132616
3	45	1.1078547842613702
3	46	0.87589703866918
3	47	1.0454032149638195
3	48	0.9820557473832672
3	49	1.0907434001898721
3	50	1.3512186831208552
3	51	1.382248588460211
3	52	1.5381695974816942
3	53	1.0780594466473374
3	54	1.482684662253849
3	55	1.4102345890484074
3	56	0.35160532882860807
4	1	-0.11878498070662857
4	2	-0.1280108723372848
4	3	-0.30918542914433544
4	4	-0.1736351363203994
4	5	-0.3843098871007484
4	6	-0.24118959586505184
4	7	-0.11293086818871245
4	8	-0.06318871850110952
4	9	-0.08655998339813209
4	10	-0.32835083431422873
4	11	-0.3784551852478444
4	12	-0.10332964112095766
4	13	-0.025970893174068976
4	14	-0.33787616387547814
4	15	-0.13154162181518103
4	16	-0.04842707207646413
4	17	-0.14879327299473744
4	18	-0.35301737930361554
4	19	-0.1344278031640087
4	20	-0.2051811622186719
4	21	-0.16171088423543978
4	22	-0.12193286482936827
4	23	-0.24297820033557826
4	24	0.16701486827455625
4	25	-0.07165945666331197
4	26	0.05038102096940378
4	27	-0.16951988506732363
4	28	0.054122207588861215
4	29	-0.06323821144892605
4	30	0.29340672067596074
4	31	0.2294175166417034
4	32	0.43577730959818695
4	33	0.2596374952772251
4	34	0.40982996175701203
4	35	0.39928192811811414
4	36	0.4130512683216949
4	37	0.20287014952192806
4	38	0.5199336739646512
4	39	0.4889742396950041
4	40	0.43986689066593243
4	41	0.7482971758143648
4	42	0.7056605236657703
4	43	0.48134515110739784
4	44	0.6225885881110784
4	45	0.5647546087910911
4	46	1.2992711607390817
4	47	0.9489153983418284
4	48	0.7987888520033657
4	49	0.8247635676968521
4	50	1.3116915731949774
4	51	1.5644749312244919
4	52	1.1156276860131626
4	53	1.5162744648656883
4	54	1.3558020606639651
4	55	1.4815269114136917
4	56	1.0102739770593203
5	1	-0.3132557459928478
5	2	-0.22026232617671582
5	3	-0.2620076426961617
5	4	-0.3098780064688498
5	5	-0.24690115849030292
5	6	-0.39619767791175775
5	7	-0.08000349513258698
5	8	-0.05482798052663185
5	9	-0.0610411531582713
5	10	-0.02998860998634001
5	11	-0.1735009835125254
5	12	-0.2693785421483952
5	13	0.10503258117704206
5	14	-0.39866801865875345
5	15	-0.182588572003759
5	16	-0.11368263196721529
5	17	0.05278411956977891
5	18	-0.7895779574950907
5	19	0.18365647307476962
5	20	-0.14252207595443878
5	21	-0.00638695208747464
5	22	-0.16487454403714707
5	23	-0.21474677510844487
5	24	-0.383121690445698
5	25	5.912539994170629E-4
5	26	0.25567894193255825
5	27	0.10735988752060166
5	28	0.330574653569208
5	29	0.4588155573859609
5	30	0.4017851090972684
5	31	0.5981399958792011
5	32	0.3447335179106119
5	33	0.5298379671944389
5	34	0.5824234626766867
5	35	0.6448925406815178
5	36	0.6992253946245768
5	37	0.2819162822161303
5	38	0.6136993503483481
5	39	0.2899608067759871
5	40	0.8157981839792043
5	41	0.7516832746940239
5	42	0.3717698078008027
5	43	0.32046597306084124
5	44	0.7325926755829784
5	45	0.8703024229225989
5	46	0.5157771154424573
5	47	0.7081261446770384
5	48	0.4968640243274649
5	49	0.5421892035774221
5	50	0.7376943972664733
5	51	0.894909875393342
5	52	0.9456213690111355
5	53	0.8221613533706034
5	54	0.7438889380212288
5	55	0.8056169844308023
5	56	0.5317703633625754
6	1	-0.09409281285611115
6	2	-0.10560987527972543
6	3	-0.17252107395195537
6	4	-0.059854376532555875
6	5	-0.11691760492596615
6	6	-0.1357910539680134
6	7	-0.017024988922635487
6	8	-0.04850460957444369
6	9	-0.09948705950810677
6	10	-0.4895152551025035
6	11	-0.4027397214848918
6	12	-0.2265374676479297
6	13	0.12042039569149665
6	14	-0.2574649713421664
6	15	-0.20177608027423588
6	16	-0.17025269945431631
6	17	2.4059322712588482E-4
6	18	-0.14315269425760135
6	19	-0.10405690524996203
6	20	-0.23269863883842135
6	21	-0.11690889012032812
6	22	-0.16926498620050978
6	23	-0.2602580324100998
6	24	-0.00400299254970804
6	25	-0.1846296386450561
6	26	0.1621611060566437
6	27	-0.11092401198600754
6	28	0.3301965745885056
6	29	0.22252092043630256
6	30	0.37559368770934043
6	31	0.5933903786841697
6	32	0.34707760759094697
6	33	0.4521380106735222
6	34	0.30060811645086005
6	35	0.6207758277001716
6	36	0.8462933921292546
6	37	0.5992426967041169
6	38	0.7287535101623348
6	39	0.9193488859457304
6	40	0.3827937793590763
6	41	0.8642988250824999
6	42	1.1139246689512987
6	43	0.7834142329523317
6	44	0.9493217252814645
6	45	1.0348991071699345
6	46	0.8310429994856321
6	47	0.9969737599438666
6	48	1.2366086319760043
6	49	1.0996572086436807
6	50	1.2038894104047166
6	51	1.0935620238271504
6	52	1.3610734569523082
6	53	1.3375397159105376
6	54	1.4739074638912832
6	55	1.5152806831774726
6	56	1.0339915773741808
7	1	-0.19830581043222395
7	2	-0.15740088736107793
7	3	-0.08987189040774268
7	4	-0.2467439395179909
7	5	0.055096462209604624
7	6	-0.06103856221829318
7	7	-0.1127436557353434
7	8	-0.013314150844863093
7	9	-0.05225096116197392
7	10	0.09644475308824241
7	11	-0.043713468228830266
7	12	-0.02678080909264935
7	13	0.09530884435407216
7	14	-0.09477026247091658
7	15	-0.12193294264584154
7	16	-0.23428340523974356
7	17	-0.17934634538511318
7	18	-0.162277141262976
7	19	-0.35966661604984296
7	20	-0.15768185256459333
7	21	-0.16405689029176074
7	22	-0.10505332200218476
7	23	-0.12284630916217765
7	24	0.0701101921754983
7	25	-0.2648989809564668
7	26	-0.005449242859747017
7	27	-0.015273204578136301
7	28	0.4957294733041806
7	29	-0.11562844644736003
7	30	0.6310736322789197
7	31	0.40001369469946724
7	32	0.25436142181332855
7	33	0.3519191938558208
7	34	0.5352587952830135
7	35	0.5173521421487521
7	36	0.5683638897172685
7	37	0.8879273195248771
7	38	0.5101997964671199
7	39	0.7738261780459368
7	40	0.6614328292321687
7	41	0.7294764738612685
7	42	0.5658397327867561
7	43	0.46174517731714104
7	44	0.8444610870697105
7	45	0.3830333306916742
7	46	0.6576098374278523
7	47	0.7922443165814705
7	48	0.545424878231831
7	49	0.7339128743087286
7	50	0.5220785881392125
7	51	0.732663089100754
7	52	0.862725892985889
7	53	0.6463988207378435
7	54	0.882367507797511
7	55	0.8683710242840803
7	56	0.9014871605602863
8	1	-0.7246008398687351
8	2	-0.7558626907244488
8	3	-0.6474974794737989
8	4	-0.870954294868767
8	5	-0.4528063954705672
8	6	-0.5776777320121909
8	7	-0.5346034864067093
8	8	-0.5280140889311085
8	9	-0.6283297809172268
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114	5	0.17345494626491842
114	6	0.15314795991238128
114	7	0.02593659564763584
114	8	0.06899610057418215
114	9	-0.025440223400785555
114	10	0.30108237709346497
114	11	0.058072110794853415
114	12	0.08152019160590385
114	13	0.28937919744207363
114	14	-0.05869918558077103
114	15	0.1091992065535905
114	16	0.05184808869029922
114	17	0.15474004677378161
114	18	0.11024759365350434
114	19	0.11595074991149801
114	20	0.0791938229940996
114	21	0.2900508071396999
114	22	0.45924784221313786
114	23	0.3802502895688846
114	24	0.1110398811690203
114	25	0.5071706723759704
114	26	0.29037302403568077
114	27	0.4476139003214712
114	28	0.45572798241602896
114	29	0.23326703387712655
114	30	0.3849917224031074
114	31	0.8292644392417898
114	32	0.6063793509456445
114	33	0.5536880605042924
114	34	1.0640347400432049
114	35	0.730539528754079
114	36	1.0907831202506557
114	37	0.8621818453923851
114	38	1.039364093543412
114	39	1.0814204496689257
114	40	0.8573162946814392
114	41	1.2840219393369878
114	42	1.234481425473831
114	43	1.4314128174696492
114	44	1.2838474663636816
114	45	1.439362451464433
114	46	1.4642777144391985
114	47	1.555553799712193
114	48	1.300930042836704
114	49	1.3625987646154307
114	50	1.5004546983398939
114	51	1.855964348300077
114	52	1.8934466051494923
114	53	1.9946354246870115
114	54	2.1155604491635245
114	55	1.9011080424356415
114	56	1.2496023292651017
115	1	-0.2324950161776087
115	2	-0.2820613910347376
115	3	-0.21080597080484154
115	4	-0.1939796297250922
115	5	-0.13196464894867432
115	6	-0.21845689811313918
115	7	-0.12029718364652098
115	8	-0.03580612362936364
115	9	-0.10211613305416734
115	10	-0.07742467410761122
115	11	0.04680392477903439
115	12	-0.059692695921945926
115	13	0.11982414852700884
115	14	-0.11980959491712184
115	15	0.007707042652256746
115	16	-0.010992774549883677
115	17	0.0520545932562726
115	18	-0.15190574835458648
115	19	-0.09549207581793695
115	20	0.07159871241551485
115	21	-0.10357165466705709
115	22	-0.04912664714353099
115	23	0.00435670205954608
115	24	-0.01885331143871838
115	25	-0.14089351262439465
115	26	-0.219509940375346
115	27	0.12366949559883977
115	28	0.19616725525747114
115	29	0.38315549257127657
115	30	0.49052488248665327
115	31	0.5739789511397362
115	32	0.6667134475101193
115	33	0.33012592543568076
115	34	0.6710536867351706
115	35	0.6766955541308022
115	36	0.727987511262107
115	37	0.7501830914173055
115	38	0.30721162228108767
115	39	0.298162560528354
115	40	0.5933564618155671
115	41	0.495829006603941
115	42	0.5300493295397217
115	43	0.5448145758855318
115	44	0.7031287209243615
115	45	0.5331589397835614
115	46	0.934101468138337
115	47	0.8985614046339911
115	48	0.9038650692595454
115	49	0.30943299056536944
115	50	0.3370917555626072
115	51	0.8511298697112224
115	52	0.6611181451308248
115	53	0.9795813342054913
115	54	1.0940591608277153
115	55	1.0989010085268411
115	56	0.6909563757187769
116	1	-0.09754462784270146
116	2	-0.3529568674078476
116	3	-0.3078317373007451
116	4	-0.1992244324006407
116	5	-0.1593971990351264
116	6	-0.07556099751897705
116	7	-0.07374755594981508
116	8	-0.056015817080321995
116	9	-0.12988288053658437
116	10	-0.16086180183514642
116	11	-0.049279676364392344
116	12	-0.20156627311661168
116	13	-0.042127169641240414
116	14	-0.12074066146310258
116	15	-0.01555235618705808
116	16	-0.18463606877431005
116	17	-0.09561626053791628
116	18	-0.051667493974875356
116	19	-0.1600242364595985
116	20	-0.06093090289984815
116	21	0.06411774646600321
116	22	-0.013332996154133525
116	23	-0.002044842894321164
116	24	0.058674318423484806
116	25	0.13743002498453194
116	26	0.20183035149860729
116	27	0.12303933002931444
116	28	0.11832145960869411
116	29	0.4156047031994916
116	30	-0.07435257435749065
116	31	-0.348328170101162
116	32	0.25870775505558186
116	33	-0.15916002668160445
116	34	0.15599013990190258
116	35	-0.05688129523866081
116	36	-0.4684971267564819
116	37	0.6279198721221562
116	38	0.2961510449951703
116	39	0.5348927041469125
116	40	0.6996805617640192
116	41	0.10470829845075968
116	42	0.31272113289846715
116	43	-0.06183262265238909
116	44	-0.34202985635662486
116	45	-0.14841260188998717
116	46	0.3837424834078256
116	47	0.28889903032797193
116	48	0.09072950843685135
116	49	0.3728565037569176
116	50	0.14301062575343337
116	51	0.6935847777267803
116	52	0.8375546389931863
116	53	0.5524696629472068
116	54	0.8111468833279574
116	55	0.5818285777155658
116	56	0.4043814920712627
117	1	-0.0421110437006007
117	2	0.05667685486082519
117	3	0.01896602844144013
117	4	-0.05069838827284201
117	5	-0.11116558259079312
117	6	-0.13857579160134748
117	7	0.0384657405497677
117	8	0.046282657469248534
117	9	0.04661621679417749
117	10	-0.23850468407618308
117	11	-0.34615728895571607
117	12	-0.421266271904976
117	13	-0.02599786378782909
117	14	-0.2871819637300774
117	15	-0.41621996793023897
117	16	-0.4252077189021364
117	17	-0.5148618956172157
117	18	-0.3392869916303525
117	19	-0.45007427704942415
117	20	-0.34831655469973555
117	21	-0.171297178473111
117	22	-0.35602137534790756
117	23	0.026073606695540263
117	24	-0.21707598751474677
117	25	-0.14790069121383453
117	26	0.07440011147340186
117	27	-0.05924290859039161
117	28	-0.07722819931561276
117	29	0.2914640564278983
117	30	0.035402311566926414
117	31	0.5085012193551819
117	32	0.4115811511516938
117	33	0.49246659539683435
117	34	0.3004172175556903
117	35	1.0719665595588097
117	36	0.43193421585798575
117	37	0.4214024790793758
117	38	0.4942330243246502
117	39	0.772293501403766
117	40	0.8904309282840757
117	41	0.5578858153227984
117	42	0.758289572189419
117	43	0.6172618001845365
117	44	0.5003132064182623
117	45	0.27288918042738786
117	46	0.276986397963217
117	47	0.41089386875624
117	48	0.17629770540048995
117	49	0.4181135524525921
117	50	0.4576823328281776
117	51	0.3295667485748055
117	52	0.47362505930991006
117	53	0.23128419773934894
117	54	0.5082704581404549
117	55	0.16797593695369173
117	56	0.4793539273395808
118	1	-0.11583609780733184
118	2	-0.282869485611009
118	3	-0.08453267419275079
118	4	-0.17390748537798117
118	5	-0.07039003285445489
118	6	-0.19825940174590784
118	7	0.020773736388626673
118	8	0.01655085945176893
118	9	0.025643132650735367
118	10	0.041501533713869776
118	11	0.07050519265995092
118	12	0.061915801728005704
118	13	0.3469571409353023
118	14	0.041898162060604704
118	15	-0.08627533017629219
118	16	0.1715769662026645
118	17	-0.016868328638466323
118	18	0.22126113047747253
118	19	0.0696719892872224
118	20	0.14740991036704543
118	21	0.2063618235715836
118	22	0.2894175169288786
118	23	0.2703622078123544
118	24	0.3636954967434818
118	25	0.2729348841111019
118	26	0.26544051515146094
118	27	0.31968476177107874
118	28	0.6162060535332117
118	29	0.35180531744354937
118	30	0.4121908230040745
118	31	0.4529667883427564
118	32	0.5943877659237273
118	33	0.7932772995615593
118	34	0.6678407805885342
118	35	0.5208623039598095
118	36	0.2912854133804359
118	37	0.7508317576961545
118	38	0.5009936752794388
118	39	-0.8149086126448566
118	40	0.37182658300541505
118	41	0.6512399543382301
118	42	0.6371060397672323
118	43	0.8774416378045586
118	44	1.0601690015181688
118	45	1.3505685098626472
118	46	1.4186539614265286
118	47	1.229439623320932
118	48	1.5184806280009617
118	49	1.316041354344236
118	50	1.5949627671169004
118	51	1.609851571188429
118	52	1.1236220699877677
118	53	1.139019367712205
118	54	1.0645904830116137
118	55	1.1426683357053484
118	56	0.8873975953574202
119	1	0.0059871026617699385
119	2	0.004428560278874727
119	3	-0.053154404431161595
119	4	-0.021461629817181915
119	5	0.08403424597451448
119	6	-0.06161232862052657
119	7	0.09457845886113603
119	8	0.009817069412534352
119	9	0.10708266202436789
119	10	0.17894951071648535
119	11	-0.05148247914107884
119	12	0.1442182096836504
119	13	0.1835417626273994
119	14	0.16125190890823404
119	15	0.2082809071010132
119	16	0.059123864368693546
119	17	0.16215514070550796
119	18	0.01798648242228751
119	19	0.09987750694286746
119	20	0.1186965529112527
119	21	-0.08582984035389529
119	22	-0.008301813935005953
119	23	-0.11318493825249476
119	24	0.02641485144378919
119	25	0.04607409359990555
119	26	-0.23478593088525201
119	27	0.1357247688266625
119	28	0.10316752910325988
119	29	0.1493554096905143
119	30	0.2324022044202465
119	31	0.3170800971722443
119	32	0.5354855763097319
119	33	0.32599279276102777
119	34	0.558069276557795
119	35	0.5263542162608346
119	36	0.32916625985956216
119	37	0.6019089257381793
119	38	0.3672856333463983
119	39	0.1362457854139052
119	40	0.5995955413059733
119	41	0.4969086375664489
119	42	0.582129709337174
119	43	0.5972039409752767
119	44	0.6298071169150674
119	45	0.6007794622231657
119	46	0.8353170526294562
119	47	0.8929718001109279
119	48	0.9973591718814383
119	49	1.0868302923361597
119	50	1.1184416456607806
119	51	1.8431386618574237
119	52	1.7717821363483743
119	53	1.696415095271405
119	54	1.7605258958346575
119	55	1.4167705367608256
119	56	1.169053559469301
120	1	-0.23541383481759937
120	2	-0.31850667442106584
120	3	-0.46739484808337295
120	4	-0.6036891336133152
120	5	-0.3553266835824971
120	6	-0.35543288714696786
120	7	-0.261300593096351
120	8	-0.3995049085368976
120	9	-0.43718352930064697
120	10	-0.20459178458951754
120	11	-0.14087311755132959
120	12	-0.4896366790732003
120	13	-0.19755680474567328
120	14	-0.28078281744021893
120	15	-0.37955826897345446
120	16	-0.3432000281512799
120	17	-0.5429349761493185
120	18	-0.3435702614873932
120	19	-0.49142770200940333
120	20	-0.37047622057524876
120	21	-0.23377992948044835
120	22	-0.3735886694179342
120	23	-0.3850209453826139
120	24	-0.4911463818037234
120	25	-0.3112573911825578
120	26	-0.1695254832718618
120	27	-0.12640634624563063
120	28	-0.31272340639552
120	29	-0.007474325570591134
120	30	0.14565235296451107
120	31	0.21758844866099025
120	32	0.35551594535971276
120	33	0.300080070769873
120	34	0.43824356849679447
120	35	0.6443313734536815
120	36	0.5752876040201187
120	37	0.6900451903450389
120	38	0.8301495667985677
120	39	0.7825453826938968
120	40	0.7848137363280898
120	41	0.8753003983111469
120	42	0.9240786101096035
120	43	0.9049216408498424
120	44	0.7831378094614436
120	45	0.9271807298009023
120	46	1.1912621242608026
120	47	1.2634411999920943
120	48	0.9138112746080509
120	49	1.1789937570486444
120	50	1.3447570876624972
120	51	1.5151199122700447
120	52	1.482979073035505
120	53	1.491754106296689
120	54	1.751184111983072
120	55	1.6919405374210221
120	56	1.7058925374847504
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	160.0
5	52.0
6	68.0
7	196.0
8	341.0
9	439.0
10	482.0
11	879.0
12	1084.0
13	1463.0
14	2306.0
15	2850.0
16	3508.0
17	4439.0
18	5792.0
19	6920.0
20	6967.0
21	8539.0
22	9025.0
23	9402.0
24	12203.0
25	14547.0
26	14726.0
27	15656.0
28	18254.0
29	19301.0
30	22640.0
31	26818.0
32	32037.0
33	40106.0
34	52896.0
35	73834.0
36	105556.0
37	149384.0
38	185441.0
39	138426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.61529769330633	0.331496639935464	0.033646250216623076	0.019559416541591122
8	99.5511926292995	0.34438641027096734	0.048155297285460544	0.05626566314406443
9	98.80626752619999	0.5882013140279527	0.21525492608466087	0.39027623368739595
10	53.45710153769444	16.46791394262098	20.69710571307248	9.377878806612095
11	37.03448841991331	35.28549147341186	12.43735666140015	15.24266344527468
12	31.02954485339052	12.815674288082842	18.81757753079088	37.33720332773576
13	25.240160245333865	12.306825425619998	21.04045961588549	41.412554713160645
14	22.32884750445154	14.066058128964457	41.29712375232712	22.307970614256888
15	38.93408273937229	15.913358878809653	23.314621829322302	21.83793655249575
16	45.609215018794266	14.631558358508903	19.51310227547969	20.24612434721714
17	28.166471917035647	14.995079742626455	35.95274120662344	20.885707133714455
18	26.85791654716505	16.064868348911617	36.46321157512083	20.614003528802506
19	42.93038570561355	18.87250604771079	17.389030714364615	20.808077532311042
20	27.82093418504319	34.95192663376983	17.035311906810538	20.191827274376443
21	25.704823068355598	18.73275249635921	35.29106540040558	20.27135903487961
22	24.563586852423697	16.11706057439824	21.932693311388952	37.386659261789106
23	25.605201791358787	17.001288083856185	19.27240997347824	38.12110015130678
24	27.54343236551195	16.1704922419219	33.64996062429496	22.63611476827119
25	42.07219042637521	15.264946244407005	17.869097072281658	24.793766256936124
26	23.07024536082056	15.638633832898268	20.824231434662057	40.46688937161911
27	22.039108383221993	15.01477137082175	19.931665295848298	43.01445495010796
28	22.929392846453915	33.741585630006135	18.97439799526208	24.354623528277862
29	41.85832137077698	18.768997114773537	17.74089407503213	21.63178743941735
30	26.225875443881673	15.962061135016231	36.44589850794796	21.366164913154137
31	27.169374021591068	30.406814808790937	21.308069205277818	21.11574196434017
32	46.15556637557648	16.054008394212893	17.770867872974762	20.01955735723587
33	29.59494811159134	16.219766499619237	19.299722536558782	34.88556285223064
34	25.216866024085604	31.815491505360377	23.867466130255888	19.10017634029813
35	23.762351054824844	20.764931143378043	38.531221513217865	16.94149628857924
36	24.532954730499917	37.416137436899525	19.176029962546817	18.874877870053737
37	25.632854381659715	20.339177989054374	34.69011992960765	19.337847699678267
38	41.417962501158286	21.38796424240141	17.632479581124112	19.5615936753162
39	28.14390655608947	17.633911517190164	17.374039487101648	36.84814243961872
40	25.15475078802608	17.674925479034226	33.64942727936289	23.520896453576803
41	24.252627199699806	18.71424754920455	33.95261761472904	23.080507636366605
42	36.335199747065246	17.028016603942927	21.09037318075656	25.54641046823527
43	20.49517769527197	15.259153604663858	37.03140288103757	27.2142658190266
44	22.014907861417697	14.816447211356582	21.51141555306615	41.65722937415957
45	23.493799453580355	16.114684849356983	36.34343811649536	24.048077580567302
46	22.138491790424577	17.85993592426894	21.426573255222745	38.57499903008374
47	21.061533480476317	31.884100897463263	23.3620299603817	23.69233566167872
48	22.553242365809616	31.153668897424968	23.694270640134643	22.59881809663077
49	24.68587574776895	30.325606387499594	21.792663691935537	23.195854172795922
50	37.494825138226304	16.037624950551322	21.640275297535386	24.827274613686985
51	22.9875378743364	31.63663118954796	22.500498178455185	22.875332757660452
52	22.253205832596507	17.66859689561942	35.952039006639104	24.126158265144973
53	23.031434891584787	15.295785647404209	38.21999108636009	23.452788374650915
54	23.25891431338409	30.126515768109375	23.45445741425672	23.160112504249817
55	22.588186925293087	31.03680681052301	20.984658118740292	25.390348145443614
56	36.193889445591935	16.299310824352236	19.64252847706497	27.864271252990857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.5
2	5.0
3	6.0
4	16.5
5	26.0
6	118.5
7	211.0
8	230.0
9	249.0
10	300.5
11	352.0
12	355.5
13	646.0
14	933.0
15	1112.0
16	1291.0
17	1454.0
18	1617.0
19	1979.0
20	2341.0
21	3061.0
22	4359.0
23	4937.0
24	5617.0
25	6297.0
26	6921.0
27	7545.0
28	7746.5
29	7372.0
30	6796.0
31	6921.5
32	7047.0
33	7107.0
34	7167.0
35	7086.5
36	7006.0
37	7595.5
38	9154.0
39	10123.0
40	15230.0
41	20337.0
42	89876.5
43	159416.0
44	94639.0
45	29862.0
46	27608.0
47	32615.5
48	39877.0
49	37166.0
50	34455.0
51	37451.0
52	40447.0
53	44404.5
54	58224.0
55	68086.0
56	79605.0
57	91124.0
58	89624.0
59	88124.0
60	75897.5
61	63671.0
62	57486.0
63	45737.5
64	40174.0
65	35038.5
66	29903.0
67	27339.5
68	24776.0
69	21355.0
70	17934.0
71	14838.5
72	9447.0
73	7151.0
74	5820.5
75	4490.0
76	3649.0
77	2808.0
78	2233.0
79	1251.0
80	844.0
81	614.0
82	384.0
83	264.0
84	144.0
85	99.0
86	54.0
87	39.0
88	16.0
89	8.0
90	5.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.03496372386968361
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0016215060345360517
21	0.0
22	0.0
23	0.0
24	0.008918283189948284
25	0.023815869882248257
26	0.06881266234062369
27	0.07175164202822029
28	0.10864090431391546
29	0.17421055458546705
30	0.25558988869374516
31	0.3037283490940342
32	0.3003839928978036
33	0.3226797008726743
34	0.3456848177376545
35	0.3876412863812748
36	0.42331441914106804
37	0.4301044756606877
38	0.4742905151017951
39	0.5168550485083664
40	0.5883026581551112
41	0.611611807401567
42	0.6330967623591697
43	0.6692766157547553
44	0.6671483890844268
45	0.6996798539023062
46	0.7368731485694769
47	0.7746745079995987
48	0.8255492598331674
49	0.7930177950152878
50	0.8563578744893522
51	0.8281842071392884
52	0.8555471214720842
53	0.8574726598880958
54	1.6323498561420113
55	0.9076380028315549
56	0.8874705215270128
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	986737.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.506203353263817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.81315119699197	17.70962972299831
2	12.807580560545432	6.789606696022517
3	6.23470163262109	4.957748079635415
4	3.5488973934047006	3.762711839578116
5	2.35416812987796	3.1200029589159
6	1.5667989816993462	2.4917935453565727
7	1.0912681272789877	2.024776242621456
8	0.8753480621880885	1.8561722992994314
9	0.6411794727419142	1.5295710141392087
>10	3.5644363084840203	17.49518213062813
>50	0.29783199198577753	5.48192161093773
>100	0.17357352856696898	9.335662573641127
>500	0.020968614189298206	3.8679448256114246
>1k	0.00815446107361597	3.556697748638329
>5k	0.001553230680688756	2.679668534720421
>10k+	3.88307670172189E-4	13.34091017725588
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	129617	13.135921729903712	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	7499	0.7599796095616157	No Hit
TTAACTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC	6994	0.7088008253465716	No Hit
TTAACTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	5974	0.6054298156448983	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	5568	0.564284100018546	No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	2719	0.2755546817439703	No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	2691	0.2727170461835322	No Hit
TTAACTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT	2605	0.2640014512479009	No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA	2583	0.26177188045041383	Illumina Single End Adapter 1 (95% over 21bp)
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	2495	0.2528535972604655	No Hit
TTAACTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	2162	0.21910600291668397	No Hit
TTAACTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	1791	0.18150733174087927	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT	1656	0.16782587457448137	No Hit
TTAACTGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC	1538	0.15586726756977798	No Hit
TTAACTGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	1442	0.14613823136256165	No Hit
TTAACTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	1399	0.141780433894746	No Hit
TTAACTGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT	1283	0.13002451514435964	No Hit
TTAACTGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAA	1231	0.12475462053211747	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC	1223	0.12394386751484944	No Hit
TTAACTGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC	1197	0.12130892020872837	No Hit
TTAACTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	1160	0.11755918750386374	No Hit
TTAACTGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT	1146	0.1161403697236447	No Hit
TTAACTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA	1082	0.1096543455855005	No Hit
TTAACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	1080	0.10945165733118349	No Hit
TTAACTGGGCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	1069	0.10833687193243995	No Hit
TTAACTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	1004	0.10174950366713723	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	998	0.10114143890418623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.080647629510194E-4	0.0	0.0	0.0	0.0
8	8.107530172680259E-4	0.0	0.0	0.0	0.0
9	8.107530172680259E-4	0.0	0.0	0.0	0.0
10	0.0020268825431700647	0.0	0.0	0.0	0.0
11	0.004357797467815639	0.0	0.0	0.0	0.0
12	0.0055739269937176775	0.0	0.0	0.0	1.0134412715850323E-4
13	0.006080647629510194	0.0	0.0	0.0	1.0134412715850323E-4
14	0.007094088901095226	0.0	0.0	0.0	1.0134412715850323E-4
15	0.008107530172680259	0.0	0.0	0.0	1.0134412715850323E-4
16	0.008715594935631277	0.0	0.0	0.0	1.0134412715850323E-4
17	0.01003306858869182	0.0	0.0	0.0	1.0134412715850323E-4
18	0.011553230496069367	0.0	0.0	0.0	1.0134412715850323E-4
19	0.01317473653060542	0.0	0.0	0.0	1.0134412715850323E-4
20	0.014796242565141471	0.0	0.0	0.0	1.0134412715850323E-4
21	0.015606995582409498	0.0	0.0	0.0	1.0134412715850323E-4
22	0.016721780981153032	0.0	0.0	0.0	1.0134412715850323E-4
23	0.017937910507055073	0.0	0.0	0.0	1.0134412715850323E-4
24	0.019356728287274118	0.0	0.0	0.0	1.0134412715850323E-4
25	0.020572857813176155	0.0	0.0	0.0	1.0134412715850323E-4
26	0.021788987339078195	0.0	0.0	0.0	1.0134412715850323E-4
27	0.02290377273782173	0.0	0.0	0.0	1.0134412715850323E-4
28	0.027160226078478865	0.0	0.0	0.0	1.0134412715850323E-4
29	0.028781732113014918	0.0	0.0	0.0	1.0134412715850323E-4
30	0.030605926401867975	0.0	0.0	0.0	1.0134412715850323E-4
31	0.03293684132651355	0.0	0.0	0.0	1.0134412715850323E-4
32	0.03486237974252511	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
33	0.03638254164990266	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
34	0.03982824197329177	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
35	0.04266587753372986	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
36	0.04590888960280196	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
37	0.04753039563733801	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
38	0.05097609596072712	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
39	0.0533070108853727	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
40	0.05604330231865229	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
41	0.05999572327783391	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
42	0.0627320147111135	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
43	0.06567099439871009	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
44	0.06820459757767268	3.040323814755097E-4	0.0	0.0	1.0134412715850323E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTAA	30	9.573141E-7	50.65854	49
ACGATTA	30	9.579944E-7	50.65332	48
TCGTACC	20	4.085253E-4	50.24163	46
ATAATCG	15	0.008394179	50.054848	33
ATGTTCG	15	0.008433107	49.996284	29
ATTCGTT	20	4.2644973E-4	49.806274	10
ACGTCGT	20	4.2644973E-4	49.806274	15
GATTAAG	14885	0.0	49.350803	50
TGGGTAC	16895	0.0	49.31511	6
CTGGGTA	17390	0.0	49.186146	5
GTAGCGT	490	0.0	48.975956	29
TAACTGG	99665	0.0	48.969143	2
ACTGGGT	20820	0.0	48.96696	4
TTAACTG	100085	0.0	48.947845	1
AACTGGG	98640	0.0	48.922466	3
GGTACCT	16675	0.0	48.90519	8
GGGTACC	16760	0.0	48.805775	7
GTACCTG	16720	0.0	48.778572	9
ACTGGGG	52730	0.0	48.649612	4
ACTGGGA	16380	0.0	48.615063	4
>>END_MODULE