FastQCFastQC Report
Fri 17 Jun 2016
SRR1526770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223821
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT127705.705452124688925No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT7440.3324084871392765No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.3225792039174162No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.26092279098029225No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT5780.2582420773743304No Hit
TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4780.21356351727496525No Hit
TAGAGAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT3540.15816210275175252No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT3110.1389503219090255No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2660.11884496986431121No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAC2600.11616425625834931No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.1108028290464255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATA1500.058.1575286
ATTACGC407.1122486E-1058.0271351
ATTAACC700.058.0271351
ACGATTA258.622863E-658.0271348
TAGTGTC150.004673627758.0271349
TATGTCT150.004673627758.0271353
GATTACG501.8189894E-1258.0271350
AGGTAAT150.004677778658.01412238
AGTATGT150.004677778658.01412245
GGTTATC202.006654E-458.001128
TAAGAAC150.004694410657.96214734
TCGCATA407.203198E-1057.94917330
TACCATA150.004698575557.94917330
CATAGGC202.0155648E-457.94917333
GCCTATG258.691877E-657.94917332
CATAAGC258.691877E-657.94917333
GCCGTTA407.203198E-1057.94917329
GCATAGG258.691877E-657.94917332
CGTAGCA950.057.936228
TCACCGT150.00471944157.88436511
Produced by FastQC (version 0.11.5)