FastQCFastQC Report
Fri 17 Jun 2016
SRR1526769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313569
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3619111.541638363486188No Hit
TAGAGAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT13380.4267003434650747No Hit
TAGAGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG11420.3641941646017304No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.346654165430894No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT7430.23694944334420814No Hit
TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6920.22068508047670526No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC6810.217177080642538No Hit
TAGAGAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC6550.2088854446708699No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6300.20091271777503517No Hit
TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC5560.17731344616336436No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.1709352646466966No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC5210.16615162850919574No Hit
TAGAGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA4850.15467090177919374No Hit
TAGAGAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG4820.15371417455169356No Hit
TAGAGAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG4730.15084399286919306No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4270.13617417538085716No Hit
TAGAGAGGGGTGTGTGGTGTGTGTATATATGTGTATATGTGGGTGGTGGTGTTATGTCTAGTGT3610.11512617637585347No Hit
TAGAGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3430.10938581301085247No Hit
TAGAGAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGT3330.10619672225251858No Hit
TAGAGAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTC3200.10205090426668453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTAT303.6714846E-758.18406353
TGAGAAC258.493846E-658.18406355
GTTAGTG150.004640348758.13745552
AACATCG150.00464623958.11883550
CTGATAT150.00464623958.11883549
TAAGATC150.00466394358.06303838
CTACGGG150.004669855358.04446836
GGTAATC150.004678734658.0166332
ACTATTG150.004711399357.9147822
GGTCCTA351.6552804E-857.914788
GGATACA150.004711399357.914788
GGCATAT150.004711399357.914788
CGTAAAC202.0227523E-457.91477621
CAACGAA150.00472630357.86860310
GTAATAC150.00472630357.8686039
TATTGGT202.0307451E-457.86860314
GATCTAT150.00472630357.8686039
ATAGTAA150.00472630357.86860318
ACGAGCC202.0307451E-457.86860317
CAATAGT150.00472630357.86860316
Produced by FastQC (version 0.11.5)