FastQCFastQC Report
Fri 17 Jun 2016
SRR1526768_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526768_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312980
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3371310.77161479966771No Hit
TAGAGAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT12800.4089718192855774No Hit
TAGAGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG11250.3594478880439645No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11050.3530577033676273No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC6970.22269793597034954No Hit
TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6670.21311265895584383No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6350.20288836347370437No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6240.19937376190171896No Hit
TAGAGAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC5860.18723241101667837No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.1722154770272861No Hit
TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4910.15687903380407694No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4770.15240590453064093No Hit
TAGAGAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG4670.14921081219247237No Hit
TAGAGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA4580.14633522908812066No Hit
TAGAGAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG4050.1294012396958272No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC3680.1175793980446035No Hit
TAGAGAGGGTACCTGGTTGATCCTGNCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3460.11055019490063263No Hit
TAGAGAGGGGTGTGTGGTGTGTGTATATATGTGTATATGTGGGTGGTGGTGTTATGTCTAGTGT3250.10384050099047862No Hit
TAGAGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3170.10128442711994376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAC258.281622E-658.43280442
TGACGCT150.00454814658.432842
AATGCGG150.00454814658.432844
TAAACTT150.00455689158.4044740
CACTAGT150.004577344758.3384524
ATCTTTA150.00458027258.32903321
ACGGGTA150.004659843658.07587454
GCGCATA150.004665778558.0572150
CCCACGA150.004665778558.0572150
CCGTCCA150.00466874858.04788649
TAATTAC150.00466874858.04788652
TTGCGAT150.004704492657.9361835
ATAATAG150.00472843557.8619532
CCATATG150.00472843557.8619532
ACGTATG150.004731434457.85268429
AGACGTA150.004740440357.82490527
TAGTCCG150.004740440357.82490527
GGTACTT303.8532562E-757.7786678
CTTATAA150.004770552757.73250617
GCGCTAA150.004770552757.73250617
Produced by FastQC (version 0.11.5)