FastQCFastQC Report
Fri 17 Jun 2016
SRR1526767_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526767_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288201
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3929913.635969340841983No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11430.3965982075010149No Hit
TAGAGAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC11430.3965982075010149No Hit
TAGAGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC9960.345592138819782No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9800.3400404578748859No Hit
TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA7880.27342028653613276No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC7810.27099142612274074No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC7770.2696035058865167No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.1838994312996832No Hit
TAGAGAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG5150.17869473041384312No Hit
TAGAGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA4860.16863230870121892No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA4830.16759136852405093Illumina Single End Adapter 1 (95% over 21bp)
TAGAGAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC4380.15197726586653065No Hit
TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA3920.13601618314995437No Hit
TAGAGAGGGGTGTGTGGTGTGTGTATATATGTGTATATGTGGGTGGTGGTGTTATG3840.1332403426775063No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC3600.12491282126016218No Hit
TAGAGAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT3480.12074906055149011No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC3320.11519737960659401No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTNAG3170.10999267872075391No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.10999267872075391No Hit
TAGAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC3080.10686985818924986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACTA150.00799358550.6686150
ACACATC203.913437E-450.66860650
CATTAAC150.0082989950.19035342
CTACATA150.0082989950.19035342
TAGCTTT150.00832195350.15528541
ACCATTA150.00833345250.13776840
TCTATTA150.00833345250.13776840
ATCAGGC204.136479E-450.10277637
GTGGCGT150.00838534450.059135
CGTCAGA150.00843167249.9893831
CGAGTAC150.00844909349.96328727
CGATACT150.00844909349.96328726
AACGTAT204.193787E-449.96328428
TATCGCT204.193787E-449.96328428
AATAGAC150.0085073649.876522
CCATAAA204.2371804E-449.85917721
AACGGGT150.0085307549.8418720
CAACGGG150.00853660549.83321819
ATCTCTA150.00854832449.8159310
CAACGAA150.00854832449.8159310
Produced by FastQC (version 0.11.5)