FastQCFastQC Report
Fri 17 Jun 2016
SRR1526766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43336
Sequences flagged as poor quality0
Sequence length64
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5221.2045412589994462No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5121.1814657559534798No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.7476462986893114No Hit
CCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2190.5053535167066642No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.36459294812626913No Hit
CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.36459294812626913No Hit
CCAGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.20306442680450434No Hit
CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.17537382314934466No Hit
CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.16383607162636146No Hit
CCAGACGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.13383791766660513No Hit
CCAGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.11306996492523537No Hit
CCAGACGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.10153221340225217No Hit
CCAGACGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.10153221340225217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCAT150.004479286358.57092758
CCATGCA700.058.57092757
CCCTGCA150.004479286358.57092757
GCCCTGC150.004479286358.57092756
CATGCAT650.058.57092358
ATCCTGG150.004626852558.09418553
AACAATA150.004626852558.09418532
CGGAGTG150.004626852558.0941856
GGGTCCG150.004626852558.0941857
AGGACTC150.004626852558.09418540
CGGGGGT258.4475E-658.0941856
GGACTCT150.004626852558.09418541
TAAGCAA150.004626852558.09418553
CACAACG150.004626852558.09418535
GACGGCT258.4475E-658.0941854
TGTTCGG150.004626852558.09418535
CGGATGT150.004626852558.0941856
CGGGCTC900.058.0941856
CGGGCTA303.635687E-758.0941856
CGGGTTA201.9727035E-458.0941856
Produced by FastQC (version 0.11.5)