FastQCFastQC Report
Fri 17 Jun 2016
SRR1526765_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526765_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50920
Sequences flagged as poor quality0
Sequence length64
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT13622.6747839748625295No Hit
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8421.6535742340926944No Hit
CCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3870.7600157109190888No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.7246661429693637No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.3436763550667714No Hit
CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.2906520031421838No Hit
CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.2258444619010212No Hit
CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.21406127258444618No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAA1040.20424194815396698No Hit
CCAGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.18067556952081695No Hit
CCAGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG870.17085624509033778No Hit
CCAGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.15318146111547526No Hit
CCAGACGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.14728986645718775No Hit
CCAGACGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.14139827179890022No Hit
CCAGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG670.13157894736842105No Hit
CCAGACGGGTCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.1296150824823252No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA640.12568735271013354No Hit
CCAGACGGGAAGTTCACGCAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.12372348782403772No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAA630.12372348782403772No Hit
CCAGACGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT630.12372348782403772No Hit
CCAGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.10997643362136685No Hit
CCAGACGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.10212097407698349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTTT150.004592496458.21859452
TCAACTT150.004592496458.21859451
AACTTTC150.004592496458.21859453
ACTTTCG150.004592496458.21859454
AGGGGGA150.004610533458.16106448
ATAGGCG150.004610533458.16106446
AGGCGAA150.004610533458.16106448
TAGGCGA150.004610533458.16106447
GAGCGTA201.993875E-457.98916226
ATCCTCC150.00470150857.87512623
CATCCTC150.00470150857.87512622
GCGGGGA202.0232316E-457.818279
GGCATTA800.057.818278
GGGCATA150.004719862757.818277
GGGTCCG150.004719862757.818277
GGGTTAC150.004719862757.818277
CGGAGCA258.712199E-657.818276
CCTATCC150.004719862757.8182710
GGCATCG150.004719862757.818278
TCGATTA150.004719862757.8182712
Produced by FastQC (version 0.11.5)