FastQCFastQC Report
Fri 17 Jun 2016
SRR1526764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50592
Sequences flagged as poor quality0
Sequence length64
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT13692.7059614168247945No Hit
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7911.5634882985452248No Hit
CCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.7352941176470588No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.6404174573055028No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.3597406704617331No Hit
CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.2767235926628716No Hit
CCAGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.2095192915876028No Hit
CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.20556609740670462No Hit
CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.20161290322580644No Hit
CCAGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG930.1838235294117647No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAA810.1601043643263757No Hit
CCAGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG780.15417457305502846No Hit
CCAGACGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.14231499051233396No Hit
CCAGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1383617963314358No Hit
CCAGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.11859582542694497No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAA580.11464263124604679No Hit
CCAGACGGGTCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.11464263124604679No Hit
CCAGACGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT570.11266603415559773No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA540.10673624288425047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCC150.004519397458.45390745
TCGTTGC150.004537379458.39539343
ATCGTTG150.004537379458.39539342
GCCAACT150.004537379458.39539343
CGTTGCT150.004537379458.39539344
CATCGTT150.004537379458.39539341
AAACTGG150.00455541558.33699839
CCATCGT150.00455541558.33699840
CCACACC150.004573503558.2787221
GCCACAC150.004573503558.2787220
CTGCCGA150.004573503558.2787221
TCTCACC150.004573503558.2787223
CTATCTC150.004573503558.2787220
CCATGCA1800.058.16251457
CATGCAT1800.058.16251458
ATGGTGG150.00460984158.1625157
GCCATGC1850.058.1625156
TGGTGGT150.00460984158.1625158
AAGCCAT1850.058.1625154
AAATTCG201.9741664E-458.1045848
Produced by FastQC (version 0.11.5)