FastQCFastQC Report
Fri 17 Jun 2016
SRR1526763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43166
Sequences flagged as poor quality0
Sequence length56
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG14903.451790761247278No Hit
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9242.1405735995922717No Hit
CCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4000.9266552379187323No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.7436408284297826No Hit
CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.4193114951582264No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.39846175230505493No Hit
CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.23166380947968307No Hit
CCAGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG900.20849742853171477No Hit
CCAGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.20849742853171477No Hit
CCAGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG880.20386415234212113No Hit
CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.16679794282537183No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAA640.14826483806699717No Hit
CCAGACGGGGCTCCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAA630.14594819997220035No Hit
CCAGACGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.1436315618774035No Hit
CCAGACGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.14131492378260668No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAA580.1343650094982162No Hit
CCAGACGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.13204837140341935No Hit
CCAGACGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT530.12278181902423202No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAA530.12278181902423202No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAA520.1204651809294352No Hit
CCAGACGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.11583190473984153No Hit
CCAGACGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.10888199045545105No Hit
CCAGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.10656535236065422No Hit
CCAGACGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC460.10656535236065422No Hit
CCAGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA450.10424871426585738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATTCG150.007986193550.60588548
AATTCGT150.007986193550.60588549
ATTCGTA150.007986193550.60588550
GAGTTTG150.00821174850.2511746
AAAATTC150.00821174850.2511747
GTAAAAT150.00821174850.2511745
CCGTGCG150.00824979650.1925341
TGCCGTG204.06087E-450.1925339
GCCGTGC204.06087E-450.1925340
GTTGCCG204.06087E-450.1925337
CGACAAA150.00844200849.90139429
TCGACAA150.00844200849.90139428
CGGAGTG204.2034197E-449.843576
TAGTGAC150.00848084749.8435718
AACTGTA150.00848084749.8435710
CGGGGGC402.6539055E-949.843576
CGGGGCG600.049.843576
CGGGGCA402.6539055E-949.843576
CGGGGAT204.2034197E-449.843576
GACGGCC204.2034197E-449.843574
Produced by FastQC (version 0.11.5)