##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526763_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43166 Sequences flagged as poor quality 0 Sequence length 56 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.66726127044433 39.0 39.0 40.0 37.0 40.0 2 38.59405550664875 39.0 39.0 40.0 36.0 40.0 3 38.13693647778344 39.0 38.0 40.0 35.0 40.0 4 38.14692118797201 39.0 38.0 40.0 35.0 40.0 5 37.68885233748784 39.0 38.0 40.0 33.0 40.0 6 38.36607515173979 39.0 38.0 40.0 35.0 40.0 7 38.596626974933976 39.0 38.0 40.0 37.0 40.0 8 38.572116943891025 39.0 38.0 40.0 37.0 40.0 9 38.441134226011215 39.0 38.0 40.0 36.0 40.0 10 37.60508270397998 39.0 38.0 40.0 33.0 40.0 11 37.6339711810221 39.0 38.0 40.0 34.0 40.0 12 37.572348607700505 39.0 38.0 40.0 33.0 40.0 13 37.30382708613261 39.0 37.0 40.0 33.0 40.0 14 37.47043969791039 39.0 37.0 40.0 33.0 40.0 15 37.33491636936478 39.0 37.0 40.0 33.0 40.0 16 37.13272019645091 39.0 36.0 40.0 33.0 40.0 17 37.18004911272761 39.0 37.0 40.0 33.0 40.0 18 37.13707547606913 39.0 36.0 40.0 33.0 40.0 19 36.86769679840615 39.0 36.0 40.0 32.0 40.0 20 37.05237918732335 39.0 36.0 40.0 33.0 40.0 21 36.90909512116017 39.0 36.0 40.0 33.0 40.0 22 36.587661585507114 38.0 36.0 40.0 31.0 40.0 23 36.50718157809387 38.0 36.0 40.0 31.0 40.0 24 36.38590557383126 38.0 35.0 40.0 31.0 40.0 25 36.309502849464856 38.0 35.0 40.0 31.0 40.0 26 36.09222536255386 38.0 35.0 40.0 30.0 40.0 27 36.074178751795394 38.0 35.0 40.0 30.0 40.0 28 35.7679423620442 38.0 35.0 40.0 29.0 40.0 29 35.90800630125562 38.0 35.0 40.0 30.0 40.0 30 35.592410693601444 38.0 35.0 39.0 29.0 40.0 31 35.4800537460038 38.0 35.0 40.0 29.0 40.0 32 35.25584951118936 38.0 35.0 40.0 28.0 40.0 33 35.32847611546124 38.0 35.0 40.0 28.0 40.0 34 35.449450956771535 39.0 35.0 40.0 29.0 40.0 35 35.14956215540008 39.0 35.0 40.0 28.0 40.0 36 35.287239957373856 39.0 35.0 40.0 29.0 40.0 37 35.13846545892601 39.0 35.0 40.0 28.0 40.0 38 35.01927442894871 39.0 35.0 40.0 27.0 40.0 39 34.969489876291526 39.0 35.0 40.0 27.0 40.0 40 34.73011166195617 39.0 35.0 40.0 26.0 40.0 41 34.57241810684335 39.0 35.0 40.0 25.0 40.0 42 34.45635453829403 39.0 35.0 40.0 24.0 40.0 43 34.30046796089515 39.0 35.0 39.0 23.0 40.0 44 34.08029467636566 39.0 34.0 40.0 22.0 40.0 45 33.82092387527221 39.0 34.0 39.0 20.0 40.0 46 33.41263957744521 39.0 34.0 39.0 8.0 40.0 47 33.19647407681972 38.0 33.0 39.0 4.0 40.0 48 33.043344298753645 38.0 33.0 39.0 4.0 40.0 49 32.756197006903584 38.0 33.0 39.0 4.0 40.0 50 32.48941296390678 38.0 33.0 39.0 4.0 40.0 51 31.741996015382476 38.0 32.0 39.0 4.0 40.0 52 31.545776768753186 37.0 32.0 39.0 4.0 40.0 53 31.202590001389982 37.0 31.0 39.0 4.0 40.0 54 30.564564703701986 37.0 31.0 39.0 4.0 40.0 55 30.123129314738453 36.0 30.0 39.0 4.0 40.0 56 17.771092989853127 21.0 4.0 25.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.0378072811189298 1 2 -1.4746380417553695 1 3 -1.450669152594621 1 4 -1.6440338906545762 1 5 -3.108469349719236 1 6 -1.0992362642775078 1 7 -0.988873353645026 1 8 -1.4489674469293945 1 9 -1.122054986652401 1 10 -0.4107256983681751 1 11 -1.7401679780651236 1 12 -1.0063729440263387 1 13 -0.5770354798619479 1 14 -0.873181109764964 1 15 -1.6454189925428935 1 16 -0.2066207137473839 1 17 -0.1596614694948002 1 18 -1.0414571893698366 1 19 -0.8398802213462915 1 20 -1.0269850550154018 1 21 -0.7426757453179889 1 22 0.04256333032243731 1 23 -0.7459040065821938 1 24 -0.5368054749289755 1 25 -1.1610342312196238 1 26 0.05727028382589339 1 27 -0.2997357748146001 1 28 -0.35866017403280637 1 29 -1.5456671123599008 1 30 -0.7965990367064677 1 31 -2.1359796789852226 1 32 -2.2433499867481643 1 33 -3.19046748942921 1 34 -4.025755362723746 1 35 -4.085727497962768 1 36 -1.7617615095158428 1 37 -3.202465004083706 1 38 -1.6481515889043976 1 39 -2.2347399587782206 1 40 -3.2562182952828422 1 41 -2.3724437002193426 1 42 -2.9536768328524623 1 43 -1.918663589847121 1 44 -3.0161876193354473 1 45 -1.5745089009396267 1 46 -1.999828794525083 1 47 -2.93689523613417 1 48 -3.80657030856878 1 49 -3.4954811231706486 1 50 -2.847545060037362 1 51 -3.0338317412517775 1 52 -2.4411160525036095 1 53 -3.652453659477633 1 54 -3.6840916579092458 1 55 -3.7658548527650666 1 56 -0.17986270516735559 2 1 0.2611029420258575 2 2 0.03859496291509856 2 3 0.09733292317081776 2 4 -0.028569438137715508 2 5 -0.03737438345042676 2 6 0.09744251102088697 2 7 0.24932073042347724 2 8 0.19192513220916396 2 9 0.3237156412666451 2 10 0.26337912778647166 2 11 -0.25340098273559164 2 12 0.46897734139140823 2 13 0.08357687094241584 2 14 0.38940321820597745 2 15 -0.33145946996894793 2 16 0.6262801684529293 2 17 0.21397630943618395 2 18 0.2896273980717865 2 19 0.1570061304959509 2 20 0.1613906585289655 2 21 0.21113847367526262 2 22 -0.39698000128109356 2 23 0.1606865486850566 2 24 -0.05886048271310074 2 25 -0.23731861108469587 2 26 -0.763694947103005 2 27 -0.8155634862832102 2 28 -0.6544567490198361 2 29 -0.5192011030189505 2 30 -0.0752705468258199 2 31 -0.34615092963390026 2 32 0.787267553469789 2 33 0.39282259879082204 2 34 -0.04495619302991827 2 35 0.22459943509379343 2 36 -0.4073245608910412 2 37 -0.8371302869067492 2 38 -0.13803223239168716 2 39 -0.6042262068321946 2 40 -1.3937377555838282 2 41 -0.4149968917087037 2 42 -0.5655086958519462 2 43 -0.13506213681131385 2 44 0.5468637558591567 2 45 -1.1702535817906892 2 46 0.34319144418794423 2 47 -0.25293052414662753 2 48 -0.6576341383560163 2 49 0.95132738746765 2 50 0.03880678220446754 2 51 0.7705273661690804 2 52 0.5080276942530091 2 53 -0.2118724451548495 2 54 1.618451154752922 2 55 1.8786703158908722 2 56 -0.5789286107200269 3 1 -0.47134386648478 3 2 -0.595979505170007 3 3 -0.3329862257653531 3 4 -0.6312290126058002 3 5 -0.7932254472802143 3 6 -0.29191919110677134 3 7 -0.523019695108438 3 8 -0.7453089103440291 3 9 -0.5885184012865494 3 10 -0.2552378934901256 3 11 -1.5438265146504904 3 12 -1.8161290415873168 3 13 -0.8105720652278023 3 14 -1.6329372073259378 3 15 -1.1027360657136285 3 16 -0.40723046984494715 3 17 -1.457832201202116 3 18 -1.114627921077151 3 19 -0.7392704652487296 3 20 -1.3513752989178371 3 21 -0.4877976965374984 3 22 -0.942192767238538 3 23 -0.20261132365536838 3 24 -0.1429030359045882 3 25 0.11640479317062358 3 26 1.5847093082161408 3 27 0.8179471520146677 3 28 1.2742666552354862 3 29 0.5866499608108313 3 30 0.29547413402524114 3 31 -0.007321142399860037 3 32 0.32982074495915015 3 33 0.611971534961036 3 34 0.2321714665445498 3 35 0.7618334776469879 3 36 0.07299458804513392 3 37 -0.7823430528641921 3 38 -1.2188832962214704 3 39 -0.17071556853431957 3 40 -0.22512073430723234 3 41 0.2525562997806574 3 42 0.3036402403182663 3 43 -0.18878554106663614 3 44 0.6527148196889385 3 45 1.2004910990603719 3 46 0.40382974206028166 3 47 0.33749500776826835 3 48 0.8184296914312199 3 49 0.2295188768293528 3 50 -0.8877889624763853 3 51 -0.774685399788364 3 52 -1.9978233695767784 3 53 -2.576234147282509 3 54 -0.8725062920555899 3 55 0.45792563503981043 3 56 -0.04754563199662343 4 1 -0.3630105331514457 4 2 -0.5733604575509545 4 3 -0.5865576543367865 4 4 -0.8705147268915141 4 5 -0.599177828232591 4 6 -0.36810966729724726 4 7 -0.14682921891796497 4 8 -0.14292795796308155 4 9 -0.344470782238929 4 10 -0.2254759887282205 4 11 -0.4509693717933416 4 12 -0.18755761301588336 4 13 0.0025231728674413034 4 14 -0.29246101684974946 4 15 -0.4241646371421979 4 16 -0.30127808889256613 4 17 0.14812017975026492 4 18 0.14727684082760817 4 19 0.6357295347512704 4 20 0.11529136774882431 4 21 0.6812499225101192 4 22 0.14709294704717735 4 23 -0.025230371274417962 4 24 0.32614458314303363 4 25 0.3842619360277695 4 26 1.126375974882805 4 27 0.23104239010990568 4 28 0.8480761790450089 4 29 0.20926900842988516 4 30 0.7347598483109579 4 31 0.4319645718858567 4 32 0.44886836400677055 4 33 -1.2880284650389626 4 34 -0.6523523429792633 4 35 -0.39292842711491716 4 36 -1.3662911262405828 4 37 -1.7454382909594344 4 38 -1.3903118676500412 4 39 -2.0576203304390788 4 40 -0.8810731152596105 4 41 -0.1343484621241018 4 42 -0.38921690253887675 4 43 -0.1614045886856843 4 44 -0.17704708507296374 4 45 -0.0030803295110573004 4 46 -0.5271226388920951 4 47 -0.579171658898396 4 48 -1.377998879997353 4 49 -0.3526239803135027 4 50 -0.7615984862859122 4 51 -0.2330187331216962 4 52 -0.29187098862439953 4 53 -0.08218652823488881 4 54 0.06677942223012678 4 55 -0.28850293638876323 4 56 -0.9725456319966241 5 1 -0.5335531688103643 5 2 -0.7372585749374494 5 3 -0.018451342044428998 5 4 -0.8306476172569646 5 5 -0.369388237977887 5 6 -0.41517500506026295 5 7 -0.46022899743402235 5 8 -0.10693681732077209 5 9 -0.44026258733305923 5 10 -0.30465649814129137 5 11 -0.9659195379063021 5 12 -0.26729183228498954 5 13 -0.6076650884836141 5 14 -1.3434023236050123 5 15 -1.3992476936206018 5 16 -1.2752537256588994 5 17 -0.8839949918997903 5 18 -0.8355581536352901 5 19 -0.771247209434776 5 20 -0.772305531475979 5 21 -1.8447744407235476 5 22 -0.6369602090990014 5 23 0.05727239727486477 5 24 -1.5022053614859843 5 25 -0.770223113806118 5 26 -0.7612209243419983 5 27 -2.565192382869057 5 28 -0.6292217168575434 5 29 -1.2569546903519537 5 30 -0.3399909822538305 5 31 -1.5613909098417196 5 32 -1.1004118131803864 5 33 -2.1484935813180357 5 34 -1.900968068339175 5 35 -1.3137479177018463 5 36 -2.2682844817223113 5 37 -1.7282732854223326 5 38 -1.6095809706400743 5 39 -1.2375760336505977 5 40 -1.4768649203537407 5 41 -0.25616463045189874 5 42 -1.2463597596817308 5 43 -0.23006461083407714 5 44 -1.0513549477529196 5 45 -1.3419507614047461 5 46 0.38871346299051623 5 47 0.3433089612566391 5 48 0.28645294724517356 5 49 -0.9605974022404169 5 50 -1.2610447764298733 5 51 -1.3764295858348774 5 52 -1.3123582532977096 5 53 -0.5151876356546019 5 54 0.24784254515371273 5 55 -0.04265576030902807 5 56 -0.14696423664778635 6 1 0.10853418229570622 6 2 -0.13317462712122818 6 3 0.03713572545415644 6 4 -0.35135096382531117 6 5 0.3305550405246649 6 6 0.047105199137128295 6 7 0.3281998170866842 6 8 0.18517889453401892 6 9 0.5120913548110124 6 10 -0.6058476495876874 6 11 0.2842222658373146 6 12 0.09118803158342104 6 13 0.44735476404049024 6 14 -0.4097664756186248 6 15 0.06189808062783442 6 16 -0.15784022594250757 6 17 0.718387310993009 6 18 0.6414696398984603 6 19 0.18451002255614668 6 20 0.09496616449679607 6 21 -0.010968428244815698 6 22 0.4571974766639002 6 23 0.2540959934178062 6 24 0.8778286714124874 6 25 0.18042918341452463 6 26 0.32556296675272733 6 27 0.017337395917103038 6 28 0.4949983625525576 6 29 1.137259716908396 6 30 0.22779120719597756 6 31 0.6445081258928269 6 32 0.29323537910548936 6 33 0.1753861691073766 6 34 -0.17209682613837884 6 35 -0.40280066869447495 6 36 0.7016531246304964 6 37 0.651193532501658 6 38 1.4006288989004787 6 39 0.9116015046364154 6 40 0.7437817047171578 6 41 0.7007270314879719 6 42 0.6560792647085165 6 43 0.008165678445564595 6 44 0.47161725871333005 6 45 0.6206130502798857 6 46 0.09773218108467319 6 47 0.6240803736219291 6 48 1.071478471919022 6 49 1.602079852439104 6 50 -0.43291091369589907 6 51 1.2588511855774875 6 52 1.4369327279841961 6 53 0.24998536491261447 6 54 0.31590834209075425 6 55 -2.3756109503260454 6 56 -0.9115700222405252 7 1 0.12865613351522143 7 2 0.029020494829993027 7 3 -0.5329862257653559 7 4 -0.6062290126058016 7 5 -0.41822544728021427 7 6 -0.6919191911067699 7 7 -0.4730196951084409 7 8 -0.5953089103440306 7 9 -0.5135184012865466 7 10 -0.605237893490127 7 11 -0.41882651465049037 7 12 -0.6411290415873125 7 13 -0.13557206522779808 7 14 0.11706279267406217 7 15 -0.10273606571362848 7 16 -0.9822304698449429 7 17 -0.4578322012021161 7 18 -0.33962792107715245 7 19 -0.1642704652487268 7 20 -0.7513752989178428 7 21 -0.9877976965374984 7 22 -0.5921927672385365 7 23 -1.8026113236553698 7 24 -0.2929030359045868 7 25 -1.783595206829375 7 26 -0.5652906917838578 7 27 -0.957052847985338 7 28 -0.9507333447645152 7 29 -0.513350039189163 7 30 0.5204741340252426 7 31 -0.1823211423998572 7 32 0.004820744959147305 7 33 0.7619715349610345 7 34 0.2071714665445441 7 35 0.3368334776469908 7 36 1.322994588045134 7 37 1.0426569471358036 7 38 0.6561167037785296 7 39 1.2542844314656776 7 40 0.9998792656927691 7 41 1.2275562997806588 7 42 0.8536402403182706 7 43 1.2362144589333681 7 44 0.5027148196889399 7 45 1.5004910990603761 7 46 2.0538297420602873 7 47 0.48749500776826693 7 48 1.06842969143122 7 49 1.8795188768293514 7 50 1.5122110375236133 7 51 2.0753146002116374 7 52 1.5521766304232223 7 53 1.7737658527174922 7 54 2.5524937079444143 7 55 2.832925635039807 7 56 1.9274543680033744 8 1 -0.23480540494632152 8 2 0.27645639226589225 8 3 -0.10670417448330483 8 4 -0.9767418331186164 8 5 -0.3336100626648246 8 6 0.45423465504707394 8 7 0.018005945917202837 8 8 -0.13377044880557065 8 9 0.10058416281601268 8 10 0.190274927022692 8 11 0.3036093827854103 8 12 0.7684863430280728 8 13 -0.1073669370226682 8 14 0.7478320234432942 8 15 0.6729049599274006 8 16 0.48443619682171857 8 17 0.3569113885414765 8 18 0.47511566866644017 8 19 0.033165432187168165 8 20 0.4870862395437001 8 21 0.4980997393599367 8 22 0.5353713353255642 8 23 0.5480297019856621 8 24 0.604532861531311 8 25 -1.1560311042652742 8 26 1.3699657184725496 8 27 0.6339727930403072 8 28 0.9909333219021477 8 29 0.9565217556826298 8 30 1.264063877614987 8 31 1.6407557806770612 8 32 0.24007715521555895 8 33 1.4812023041918039 8 34 0.9135817229548024 8 35 0.8067052725187835 8 36 1.6941484341989792 8 37 1.7106056650845218 8 38 1.5400910627528859 8 39 2.2299254571067095 8 40 2.3203920862055867 8 41 2.1147357869601464 8 42 2.2921017787798093 8 43 1.8349324076513156 8 44 1.9969455889197079 8 45 2.169080842650118 8 46 2.597419485650029 8 47 2.6003155205887793 8 48 3.0908655888671177 8 49 3.248108620419096 8 50 3.7878520631646424 8 51 4.728519728416767 8 52 5.493843297089885 8 53 3.375688929640571 8 54 4.237109092559798 8 55 4.872669224783401 8 56 5.142838983387993 9 1 -0.3009950292754766 9 2 -0.5512120633095421 9 3 -0.8324048304165217 9 4 -0.5748336637685867 9 5 0.0027047852779276127 9 6 0.02668546005601513 9 7 -0.46022899743402235 9 8 -0.2697275149951963 9 9 -0.114681191984225 9 10 -0.14186580046686714 9 11 0.3131502295355588 9 12 0.22108026073826892 9 13 0.7644279347722005 9 14 -0.08758837011663445 9 15 0.36819416684451056 9 16 -0.228742097751919 9 17 -0.20957638724862448 9 18 -0.3471860606120316 9 19 0.0659620928908069 9 20 -0.5397473919410984 9 21 -0.1703558360723818 9 22 -0.17184393002923315 9 23 0.12703983913532824 9 24 0.21872487107215477 9 25 -0.7237114858991447 9 26 -0.08680231969083252 9 27 0.3650401752704795 9 28 0.34752246918897356 9 29 0.3011848445317611 9 30 0.4274508782112889 9 31 0.7176788576001414 9 32 1.3181928379824086 9 33 0.9677854884494081 9 34 0.8897296060794275 9 35 0.9885776636934978 9 36 1.3131108671149008 9 37 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26 1.6118832212596175 120 27 1.5364254128842276 120 28 1.6068753508876554 120 29 1.0312151782021388 120 30 -0.10996064858345278 120 31 0.7394179880349228 120 32 -0.2136575159104126 120 33 -0.26628933460418125 120 34 0.07021494480541435 120 35 -0.33599260930953534 120 36 0.8262554576103511 120 37 -0.2247343572120215 120 38 0.5072036603002701 120 39 0.7042844314656804 120 40 0.34770535264929237 120 41 0.3014693432589155 120 42 -0.3767945422904262 120 43 0.34273619806380395 120 44 0.01032351534111342 120 45 0.055925881669068644 120 46 0.6364384377124566 120 47 1.0440167468987056 120 48 0.9325601262138292 120 49 1.3306058333510933 120 50 1.5665588636105667 120 51 2.2307493828203313 120 52 1.8489157608580022 120 53 2.103113678804444 120 54 2.504667620987888 120 55 1.804664765474591 120 56 1.3535413245251142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 39.0 5 3.0 6 6.0 7 16.0 8 33.0 9 52.0 10 32.0 11 50.0 12 60.0 13 66.0 14 91.0 15 109.0 16 129.0 17 142.0 18 190.0 19 215.0 20 226.0 21 270.0 22 279.0 23 280.0 24 354.0 25 371.0 26 404.0 27 476.0 28 605.0 29 718.0 30 839.0 31 1180.0 32 1432.0 33 1782.0 34 2373.0 35 2929.0 36 4006.0 37 6873.0 38 8920.0 39 7616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.2007598572951 0.5907427141731919 0.11583190473984153 0.09266552379187323 8 98.9758561564484 0.6835349182075166 0.1853654015478011 0.15524352379628342 9 93.52036324885327 3.4124079136357315 1.248667933095492 1.8185609044155122 10 27.757957651855627 40.112588611407126 14.133809016355464 17.995644720381783 11 20.247416948524304 39.56122874484548 19.8118889867025 20.37946531992772 12 39.56586202103507 15.813371635083168 24.336283185840706 20.28448315804105 13 22.501505814761618 16.248899596904973 25.2189222999583 36.03067228837511 14 24.264467404902007 27.5587267757031 23.300745957466525 24.876059861928372 15 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29.382040702795422 28 18.022864828513786 29.081928437260856 35.7581800895114 17.137026644713956 29 24.054950920103032 20.8943447891769 36.024412317545774 19.0262919731743 30 13.768839553191983 25.7541626994264 43.75885371914261 16.71814402823901 31 16.309601264058 27.41890510270471 42.50395018124361 13.76754345199368 32 15.100257905620484 22.565580055298682 48.83013081158949 13.504031227491344 33 13.356108670896372 22.087429408073625 45.153268725743104 19.4031931952869 34 14.633749447892693 26.380268266034356 47.09533440267801 11.890647883394937 35 11.536404436899751 25.644722461223633 51.88475222658884 10.93412087528777 36 10.999976739317532 27.021934823567722 48.01004861482636 13.968039822288386 37 11.361310133060389 21.16637201079371 55.34102540243789 12.131292453708012 38 14.677682103793344 22.469164533395393 51.405631836164766 11.447521526646497 39 10.081255384042281 22.8073851598333 50.92780144816186 16.18355800796256 40 10.414093631300538 22.610863840794167 56.34656164798546 10.628480879919838 41 11.036781502027877 23.218031793389585 55.27947415038926 10.465712554193278 42 15.390355377296894 21.320305941609924 51.5320399216491 11.757298759444081 43 12.922186975181937 21.87674939354357 55.700690427318534 9.500373203955961 44 11.709274118305654 22.2196305280836 53.07426758723642 12.996827766374325 45 11.017779644407112 24.550842316487003 55.462224088851556 8.969153950254329 46 9.726663709997432 24.056394575290028 51.89421348708013 14.322728227632409 47 10.188925994255154 28.633147287545828 52.76383083066719 8.414095887531818 48 11.958502733772606 28.615823169306974 50.60516846581616 8.820505631104258 49 11.213359803807077 28.71190003503445 50.706528085951184 9.368212075207287 50 14.648341631021665 25.161863356940838 51.04363882850665 9.146156183530842 51 10.127114683615291 28.944293859239178 51.76652023553603 9.162071221609496 52 10.057968304427096 26.218970595110093 52.718432985835165 11.004628114627648 53 10.975780811670095 27.2512623901253 52.723489807368615 9.049466990835983 54 10.720678285445125 31.733396137541213 48.50918511540273 9.036740461610927 55 9.896613023952096 32.37743263473054 47.36386601796407 10.362088323353294 56 14.830924652729058 28.614190168841496 47.09087507600206 9.46401010242739 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 3.0 8 4.0 9 5.0 10 7.0 11 9.0 12 45.0 13 91.0 14 101.0 15 102.5 16 104.0 17 159.5 18 215.0 19 472.5 20 730.0 21 894.0 22 1147.5 23 1237.0 24 1435.5 25 1634.0 26 1953.5 27 2273.0 28 2567.0 29 2987.0 30 3113.0 31 3109.5 32 3106.0 33 3000.0 34 2894.0 35 2255.0 36 1616.0 37 1763.0 38 1340.0 39 770.0 40 760.5 41 751.0 42 759.5 43 768.0 44 772.0 45 776.0 46 912.5 47 1806.0 48 2563.0 49 1785.0 50 1007.0 51 983.5 52 960.0 53 1031.0 54 1329.5 55 1557.0 56 1676.5 57 1796.0 58 1610.0 59 1424.0 60 1388.0 61 1352.0 62 1245.5 63 1033.0 64 927.0 65 773.5 66 620.0 67 606.0 68 592.0 69 462.0 70 332.0 71 285.5 72 214.5 73 190.0 74 157.5 75 125.0 76 97.5 77 70.0 78 54.0 79 37.0 80 36.0 81 23.5 82 11.0 83 11.0 84 11.0 85 8.0 86 5.0 87 5.5 88 3.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.018533104758374646 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0023166380947968307 21 0.0 22 0.0 23 0.0 24 0.0046332761895936615 25 0.016216466663577817 26 0.06486586665431127 27 0.05559931427512394 28 0.09961543807626373 29 0.16911458092016865 30 0.24324699995366722 31 0.30116295232358803 32 0.2942130380391975 33 0.3173794189871658 34 0.3451790761247278 35 0.3776120094518834 36 0.4054116665894454 37 0.4123615808738359 38 0.4540610665801788 39 0.4980771903813186 40 0.5861094379835982 41 0.6115924570263633 42 0.6532919427327063 43 0.6810915998702682 44 0.6810915998702682 45 0.7135245331974239 46 0.75290738080897 47 0.7992401427049066 48 0.8525228188852337 49 0.8131399712736875 50 0.8872723903071863 51 0.8571560950748274 52 0.8895890284019831 53 0.903488856970764 54 1.6355464949265626 55 0.9590881712458879 56 0.9359217902979197 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 43166.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.312004818607235 #Duplication Level Percentage of deduplicated Percentage of total 1 73.69912758513794 38.55349117360886 2 13.037509410566406 13.64036510216374 3 5.491342278907045 8.61789371264421 4 2.6393870953456444 5.522865217995645 5 1.417120588105044 3.706620951674929 6 0.9078428767547938 2.8494648566001017 7 0.5934192462689872 2.1730065329194272 8 0.5314202205393915 2.223972571004958 9 0.3099951286479784 1.4594819997220034 >10 1.2842655329701962 10.941481721725433 >50 0.061999025729595675 2.230922485289348 >100 0.017714007351313052 2.4880693138117964 >500 0.004428501837828263 2.1405735995922717 >1k 0.004428501837828263 3.451790761247278 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1490 3.451790761247278 No Hit CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 924 2.1405735995922717 No Hit CCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 400 0.9266552379187323 No Hit CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 321 0.7436408284297826 No Hit CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 181 0.4193114951582264 No Hit CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 172 0.39846175230505493 No Hit CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 100 0.23166380947968307 No Hit CCAGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 90 0.20849742853171477 No Hit CCAGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 90 0.20849742853171477 No Hit CCAGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 88 0.20386415234212113 No Hit CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.16679794282537183 No Hit CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAA 64 0.14826483806699717 No Hit CCAGACGGGGCTCCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAA 63 0.14594819997220035 No Hit CCAGACGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.1436315618774035 No Hit CCAGACGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.14131492378260668 No Hit CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAA 58 0.1343650094982162 No Hit CCAGACGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57 0.13204837140341935 No Hit CCAGACGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 53 0.12278181902423202 No Hit CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAA 53 0.12278181902423202 No Hit CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAA 52 0.1204651809294352 No Hit CCAGACGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50 0.11583190473984153 No Hit CCAGACGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47 0.10888199045545105 No Hit CCAGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.10656535236065422 No Hit CCAGACGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 46 0.10656535236065422 No Hit CCAGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 45 0.10424871426585738 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.011583190473984154 0.0 0.0 0.0 0.0 11 0.016216466663577817 0.0 0.0 0.0 0.0 12 0.016216466663577817 0.0 0.0 0.0 0.0 13 0.018533104758374646 0.0 0.0 0.0 0.0 14 0.03706620951674929 0.0 0.0 0.0 0.0 15 0.039382847611546125 0.0 0.0 0.0 0.0 16 0.04633276189593662 0.0 0.0 0.0 0.0 17 0.04864939999073345 0.0 0.0 0.0 0.0 18 0.050966038085530276 0.0 0.0 0.0 0.0 19 0.05559931427512394 0.0 0.0 0.0 0.0 20 0.06254922855951443 0.0 0.0 0.0 0.0 21 0.0671825047491081 0.0 0.0 0.0 0.0 22 0.08339897141268592 0.0 0.0 0.0 0.0 23 0.10193207617106055 0.0 0.0 0.0 0.0 24 0.11119862855024788 0.0 0.0 0.0 0.0 25 0.11119862855024788 0.0 0.0 0.0 0.0 26 0.11119862855024788 0.0 0.0 0.0 0.0 27 0.11814854283463837 0.0 0.0 0.0 0.0 28 0.16911458092016865 0.0 0.0 0.0 0.0 29 0.17838113329935598 0.0 0.0 0.0 0.0 30 0.20154751424732428 0.0 0.0 0.0 0.0 31 0.20849742853171477 0.0 0.0 0.0 0.0 32 0.2177639809109021 0.0 0.0 0.0 0.0 33 0.22239725710049577 0.0 0.0 0.0 0.0 34 0.23629708566927674 0.0 0.0 0.0 0.0 35 0.2710466570912292 0.0 0.0 0.0 0.0 36 0.28031320947041655 0.0 0.0 0.0 0.0 37 0.29652967613399434 0.0 0.0 0.0 0.0 38 0.30347959041838485 0.0 0.0 0.0 0.0 39 0.3104295047027753 0.0 0.0 0.0 0.0 40 0.32896260946114997 0.0 0.0 0.0 0.0 41 0.34981235231432145 0.0 0.0 0.0 0.0 42 0.3613955427883056 0.0 0.0 0.0 0.0 43 0.3683454570726961 0.0 0.0 0.0 0.0 44 0.37529537135708657 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAATTCG 15 0.0079861935 50.605885 48 AATTCGT 15 0.0079861935 50.605885 49 ATTCGTA 15 0.0079861935 50.605885 50 GAGTTTG 15 0.008211748 50.25117 46 AAAATTC 15 0.008211748 50.25117 47 GTAAAAT 15 0.008211748 50.25117 45 CCGTGCG 15 0.008249796 50.19253 41 TGCCGTG 20 4.06087E-4 50.19253 39 GCCGTGC 20 4.06087E-4 50.19253 40 GTTGCCG 20 4.06087E-4 50.19253 37 CGACAAA 15 0.008442008 49.901394 29 TCGACAA 15 0.008442008 49.901394 28 CGGAGTG 20 4.2034197E-4 49.84357 6 TAGTGAC 15 0.008480847 49.84357 18 AACTGTA 15 0.008480847 49.84357 10 CGGGGGC 40 2.6539055E-9 49.84357 6 CGGGGCG 60 0.0 49.84357 6 CGGGGCA 40 2.6539055E-9 49.84357 6 CGGGGAT 20 4.2034197E-4 49.84357 6 GACGGCC 20 4.2034197E-4 49.84357 4 >>END_MODULE