FastQCFastQC Report
Fri 17 Jun 2016
SRR1526762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105761
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.5313868061005474No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5570.5266591654768772No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.2987868874159662No Hit
GAGGAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2470.23354544680931535No Hit
GAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.19761537806942067No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.18154139994894147No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.17681375932527113No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1510.1427747468348446No Hit
GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.13521052183697205No Hit
GAGGAGGGGGTATTACATGTGCATATGTCTTAGATTTTCTTTTGATAAACTGTTAAGCTAAATA1160.10968126246915214No Hit
GAGGAGGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTACACTCAAG1160.10968126246915214No Hit
GAGGAGGGGTATACTTAATAAAGTTACTGCTGTCTGTCAGATAAAAAAAAAAAAAAAAAAAAAA1140.107790206219684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGT150.004497793558.56992358
GCTAAAT150.004497793558.56992357
ATGTTGT201.9074195E-458.56992356
TGATACA150.004653380358.0693345
CAAATTG150.004653380358.0693349
CATACTA258.554482E-658.0693345
TAAGCTA150.004653380358.0693354
AAGCTAA150.004653380358.0693355
CCCGAGT150.004653380358.0693341
AAGATTA900.058.0693248
ATCCTAT150.0046621458.04176738
CAGAAAA150.0046621458.04176738
TGGACTA150.0046621458.04176735
ATCAGTC201.9948519E-458.04176731
GATCCTA150.0046621458.04176737
AGAATTC150.0046621458.04176735
TTACCAA201.9995403E-458.01423329
CTTAGAT150.00467091258.01423329
TCAATCA150.00467969657.98672528
ACACAGT150.004706122457.90435824
Produced by FastQC (version 0.11.5)