FastQCFastQC Report
Fri 17 Jun 2016
SRR1526760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147850
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT13150.8894149475820089No Hit
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8400.5681433885694962No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC5130.3469732837335137No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.2448427460263781No Hit
GAGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3580.2421373013189043No Hit
GAGGAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.2367264119039567No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3390.22928643895840378No Hit
GAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3210.21711193777477172No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3070.20764288129861344No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2880.19479201893811296No Hit
GAGGAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2560.17314846127832262No Hit
GAGGAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2300.15556307067974298No Hit
GAGGAGGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTACACTCAAG2050.1386540412580318No Hit
GAGGAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2050.1386540412580318No Hit
GAGGAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1900.1285086236050051No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.1285086236050051No Hit
GAGGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1850.12512681772066284No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1840.12445045654379439No Hit
GAGGAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1680.11362867771389923No Hit
GAGGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1640.11092323300642543No Hit
GAGGAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1640.11092323300642543No Hit
GAGGAGGGGGAGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.1088941494758201No Hit
GAGGAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1600.10821778829895165No Hit
GAGGAGGGGGGTCTTCACACTCACGGTCCACACCTCCCCGAGGTCCTGTTCCCTAGTGGTCCAC1590.10754142712208319No Hit
GAGGAGGGGAGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.10686506594521474No Hit
GAGGAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1530.10348326006087251No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1510.10213053770713561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAA201.927058E-458.4643645
GATAAAC201.930327E-458.44433244
CGTGATT150.00453979758.44433243
GTGCGAA150.00453979758.44433243
CCCGATT258.2501665E-658.4443343
TATATAC201.9336003E-458.42431639
TCGTCGT150.004545960658.42431641
ACCCGAT258.266947E-658.42431342
GATCGGT150.004552130658.40431626
CGCGATC150.004558306758.38432725
TCGATAC201.9401601E-458.38432725
GCAATAG150.00456448958.36435721
CTCGATA201.9434467E-458.36435324
ACGGTCC406.730261E-1058.36435323
CGGATGT150.004570677458.34439520
CACTCAA150.004651698358.08614357
CCCAAAC150.004651698358.08614357
ACTCAAG150.004651698358.08614358
CCAAACA150.004651698358.08614358
CGTTATG201.9899252E-458.0861458
Produced by FastQC (version 0.11.5)