FastQCFastQC Report
Fri 17 Jun 2016
SRR1526759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132597
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG14461.0905224100092763No Hit
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8950.6749775635949531No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7300.5505403591333137No Hit
GAGGAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.3167492477205367No Hit
GAGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG3930.2963867960813593No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3530.2662202010603558No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.24359525479460317No Hit
GAGGAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2870.21644531927570007No Hit
GAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2760.20814950564492407No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2740.20664117589387393No Hit
GAGGAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT2680.20211618664072337No Hit
GAGGAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2490.18778705400574672No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.1478163156029171No Hit
GAGGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1910.14404549122529167No Hit
GAGGAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1820.1372580073455659No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1820.1372580073455659No Hit
GAGGAGGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT1790.13499551271899063No Hit
GAGGAGGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTA1750.1319788532168903No Hit
GAGGAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1710.12896219371478992No Hit
GAGGAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA1690.12745386396373976No Hit
GAGGAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG1660.1251913693371645No Hit
GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.1236830395861143No Hit
GAGGAGGGGGATACTGGTCCTGAAGTCTACCAAATATTATAGTGCATTTTAGCCTA1640.1236830395861143No Hit
GAGGAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1640.1236830395861143No Hit
GAGGAGGGGGAGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1590.1199122152084889No Hit
GAGGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1540.11614139083086344No Hit
GAGGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1530.11538722595533836No Hit
GAGGAGGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT1460.11010807182666275No Hit
GAGGAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1460.11010807182666275No Hit
GAGGAGGGGAGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.10256642307141187No Hit
GAGGAGGTGGCTTAGAGCTGTCTTAGTCACTAAAGCATGGGAGCAGTGTGAACTCT1360.10256642307141187No Hit
GAGGAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1330.10030392844483661No Hit
GAGGAGGGGGGTCTTCACACTCACGGTCCACACCTCCCCGAGGTCCTGTTCCCTAG1330.10030392844483661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCT150.00799250850.65490749
TAGCCTA251.9233177E-550.65490750
ACTACTA150.00799250850.65490750
AGAATCA150.00799250850.65490748
GCACTAC150.00799250850.65490748
ACGGGGT150.00823766850.26940545
TCAGCAC150.00823766850.26940545
CATACTA150.00823766850.26940545
ACATGCT150.00823766850.26940545
TCACGTG150.00823766850.26940547
TACGAGC150.00825007250.25028644
GATAAAC204.0687976E-450.25028244
TACCTCA252.0259513E-550.21208643
GTTGACA150.00831229550.15489641
TTTGATA204.107149E-450.15489641
TGTGACG150.00833728250.11684439
TGTTGAC150.00833728250.11684440
TAGTGCA252.0488527E-550.11684440
CGTGTGA150.00834979750.0978437
TAAGATC204.1302975E-450.09783637