FastQCFastQC Report
Fri 17 Jun 2016
SRR1526758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65757
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT30774.67934972702526No Hit
GCTCAAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT2060.3132746323585322No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT1390.21138433930988337No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.19161458095716047No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.18705232902960903No Hit
GCTCAAGGACGTTTTTGAGACTGTATTCCCCAGCATATATCTCCTAACCTTTGAAGACTCCATA1050.15967881746430038No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC780.11861855011633743No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.11405629818878597No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT730.1110147969037517No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT710.10797329561871741No Hit
GCTCAAGGGATGGCTTAACCTTGGCTTCCTGAACCAGTCCCTCCCTAGTCTATCTTCTATCCCC700.10645254497620026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCAG150.004489761358.57121358
CCTGCAT201.9018189E-458.5712158
AAGGGTC750.058.079395
AGGGTCT258.5097945E-658.0793886
AGGGTAT501.8189894E-1258.0793886
GGACGTT201.982892E-458.0793847
GGTGTAT150.00465641558.0350888
GACGTTT201.9903964E-458.0350848
GGGAGTA201.9903964E-458.0350848
GGTATAG406.9849193E-1058.0350848
ACTATTG150.004670507757.99085649
AGCAAGC351.6114427E-857.99085648
CTGTCGA150.004670507757.99085612
ATCTCCC150.004670507757.99085636
ACGCCAT150.004670507757.99085654
ACGCCAA150.004670507757.99085644
CTCGTAA150.004670507757.99085639
CTCAACG150.004670507757.99085644
AAGAGAC150.004670507757.99085650
TTTCGCA150.004670507757.99085614
Produced by FastQC (version 0.11.5)