FastQCFastQC Report
Fri 17 Jun 2016
SRR1526757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95669
Sequences flagged as poor quality0
Sequence length64
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT71547.477866393502596No Hit
GCTCAAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT6710.7013766214761312No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC5070.5299522311302511No Hit
GCTCAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG4380.45782855470424066No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2590.2707251042657496No Hit
GCTCAAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG2330.24354806677189056No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2130.22264265331507593No Hit
GCTCAAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG2100.21950684129655373No Hit
GCTCAAGGGTATAGGAACCTGGGGCCCAGCTGGCAGGAGAGGAGGTTTGGGAACCGGGGCCCGG2100.21950684129655373No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2090.218461570623713No Hit
GCTCAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2080.21741629995087225No Hit
GCTCAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1970.20591832254962422No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.19964669851257982No Hit
GCTCAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1870.1954656158212169No Hit
GCTCAAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC1690.17665074371008374No Hit
GCTCAAGGACGTTTTTGAGACTGTATTCCCCAGCATATATCTCCTAACCTTTGAAGACTCCATA1660.17351493169156154No Hit
GCTCAAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT1650.1724696610187208No Hit
GCTCAAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG1460.1526095182347469No Hit
GCTCAAGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG1350.14111154083349883No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.1400662701606581No Hit
GCTCAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA1330.13902099948781738No Hit
GCTCAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1290.13483991679645446No Hit
GCTCAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1260.13170410477793226No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1250.13065883410509152No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.12856829275941006No Hit
GCTCAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1220.12752302208656932No Hit
GCTCAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1190.12438721006804712No Hit
GCTCAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1170.12229666872236565No Hit
GCTCAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1160.12125139804952491No Hit
GCTCAAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1150.12020612737668421No Hit
GCTCAAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG1150.12020612737668421No Hit
GCTCAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1110.11602504468532127No Hit
GCTCAAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCCTTCGTTGC1100.11497977401248054No Hit
GCTCAAGGGCAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGTCACTCTCCTG1080.11288923266679907No Hit
GCTCAAGGGATGGCTTAACCTTGGCTTCCTGAACCAGTCCCTCCCTAGTCTATCTTCTATCCCC1040.10870814997543612No Hit
GCTCAAGGGACACTAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTCCCT1030.1076628793025954No Hit
GCTCAAGGGGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGG1010.10557233795691395No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1000.10452706728407322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT150.004622148358.16386856
AGCGTTC150.004622148358.16386856
TATTCTC150.004622148358.16386855
ACTGTGC150.004622148358.16386854
AGACAAT150.004622148358.16386854
ACGTCTT150.004622148358.16386858
ATTCTCT150.004622148358.16386856
TAACTCT150.004622148358.16386853
TCGTTGC201.9732665E-458.16386458
GTGGTTT201.9732665E-458.16386456
CCAGCGT150.00463177458.13333551
GTTAACT150.00463177458.13333551
CCTCAAT303.6663005E-758.13333551
CTAGATT150.004641414658.10283346
CAACCAA150.004641414658.10283349
TATCTTA150.004641414658.10283346
CTAGTTT150.004641414658.10283346
CTAAACG150.004641414658.10283346
CGATCCA150.004641414658.10283346
AAGTGCC150.004641414658.10283348
Produced by FastQC (version 0.11.5)