FastQCFastQC Report
Fri 17 Jun 2016
SRR1526756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95775
Sequences flagged as poor quality0
Sequence length64
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT69587.264943878882798No Hit
GCTCAAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT6300.6577916992952232No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4720.4928217175672148No Hit
GCTCAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG3950.41242495432002085No Hit
GCTCAAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG2230.2328373792743409No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2220.2317932654659358No Hit
GCTCAAGGGTATAGGAACCTGGGGCCCAGCTGGCAGGAGAGGAGGTTTGGGAACCGGGGCCCGG2210.23074915165753068No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2170.22657269642391023No Hit
GCTCAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2010.20986687548942834No Hit
GCTCAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1910.1994257374053772No Hit
GCTCAAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG1820.19002871312973113No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1800.18794048551292092No Hit
GCTCAAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT1790.1868963717045158No Hit
GCTCAAGGACGTTTTTGAGACTGTATTCCCCAGCATATATCTCCTAACCTTTGAAGACTCCATA1710.17854346123727488No Hit
GCTCAAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC1670.1743670060036544No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.1670582093448186No Hit
GCTCAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1580.16496998172800836No Hit
GCTCAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1420.1482641607935265No Hit
GCTCAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA1240.1294701122422344No Hit
GCTCAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1210.12633777081701905No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.1232054293918037No Hit
GCTCAAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG1150.12007308796658836No Hit
GCTCAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1140.11902897415818324No Hit
GCTCAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1140.11902897415818324No Hit
GCTCAAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG1110.1158966327329679No Hit
GCTCAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1110.1158966327329679No Hit
GCTCAAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCCTTCGTTGC1080.11276429130775253No Hit
GCTCAAGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG1070.11172017749934743No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.11067606369094231No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1010.10545549464891672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCT150.004521752258.48702644
TGCGAAA150.004521752258.48702644
TCGTAAT150.004521752258.48702644
CGTAATT150.004521752258.48702645
CCCTAGT201.9249125E-458.45608543
CCTAACC201.9249125E-458.45608543
GTGCGAA150.00453124458.4560843
CGAAGGG150.00453124458.4560842
TATCCAG150.00453124458.4560839
ATTCCCC201.9350131E-458.3942925
GACTTTA201.9400791E-458.36344523
TGGTGAT150.0045693658.33262621
GCGAGTT150.0045693658.33262621
CCTTCGT150.00464630858.08727655
TCGTTGC150.00464630858.08727658
CATGACA150.00464630858.08727654
GATTTAC150.00464630858.08727657
AAGTCCT150.00464630858.08727657
TCTATCC201.9861502E-458.08727356
AACGTCA150.004655993558.0567549
Produced by FastQC (version 0.11.5)