SRR1526755_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526755_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87270
Sequences flagged as poor quality	0
Sequence length	56
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	7642	8.756731981207746	No Hit
GCTCAAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC	565	0.6474160650853673	No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	370	0.42397158244528477	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	331	0.37928268591726827	No Hit
GCTCAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	330	0.37813681677552424	No Hit
GCTCAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	269	0.3082387991291395	No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	261	0.2990718459951873	No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	210	0.24063251976624267	No Hit
GCTCAAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT	183	0.20969405293915436	No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	181	0.20740231465566633	No Hit
GCTCAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	168	0.19250601581299415	No Hit
GCTCAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT	167	0.19136014667125015	No Hit
GCTCAAGGGGAGACCTGGCCGGTGCCCTGCGCGGGGCCTCCCCAGGACACACTGAC	162	0.1856308009625301	No Hit
GCTCAAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	156	0.178755586112066	No Hit
GCTCAAGGGTATAGGAACCTGGGGCCCAGCTGGCAGGAGAGGAGGTTTGGGAACCG	155	0.177609716970322	No Hit
GCTCAAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	147	0.16844276383636989	No Hit
GCTCAAGGACGTTTTTGAGACTGTATTCCCCAGCATATATCTCCTAACCTTTGAAG	143	0.16385928726939383	No Hit
GCTCAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	135	0.15469233413544173	No Hit
GCTCAAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	134	0.1535464649936977	No Hit
GCTCAAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG	130	0.14896298842672168	No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	123	0.14094190443451357	No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA	119	0.13635842786753752	Illumina Single End Adapter 1 (95% over 21bp)
GCTCAAGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT	118	0.13521255872579352	No Hit
GCTCAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	112	0.12833734387532944	No Hit
GCTCAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	111	0.12719147473358544	No Hit
GCTCAAGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT	110	0.12604560559184141	No Hit
GCTCAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA	109	0.1248997364500974	No Hit
GCTCAAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA	108	0.12375386730835339	No Hit
GCTCAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	106	0.12146212902486536	No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105	0.12031625988312133	No Hit
GCTCAAGGGCTATTCTGAAAGCGACTTTAATTTGACTGGTATGAATTGTGGCTTAA	104	0.11917039074137734	No Hit
GCTCAAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC	102	0.11687865245788931	No Hit
GCTCAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	101	0.1157327833161453	No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	98	0.11229517589091327	No Hit
GCTCAAGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAA	98	0.11229517589091327	No Hit
GCTCAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG	98	0.11229517589091327	No Hit
GCTCAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	94	0.10771169932393719	No Hit
GCTCAAGGGGCGGGGTCTCCTGTGAGCAGCCTCCATGAGGCCCTGGACCAGTGCAT	93	0.10656583018219319	No Hit
GCTCAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	92	0.10541996104044918	No Hit
GCTCAAGGGGTTGGCGCTGCGGTGGCGTTTCCTACTGTGGTAGCCTCCGCTTCCCA	92	0.10541996104044918	No Hit
GCTCAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	91	0.10427409189870518	No Hit
GCTCAAGGGGTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT	89	0.10198235361521715	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGTGA	15	0.00802489	50.588375	50
TCATACC	15	0.008286413	50.17993	45
GCGAGGG	25	2.0347825E-5	50.15101	42
TACCAGC	15	0.008362284	50.064445	39
ATACTTT	15	0.008362284	50.064445	37
ACGGCGT	15	0.008362284	50.064445	37
GGTGGAT	15	0.008362284	50.064445	39
GTGAGCC	15	0.008362284	50.064445	38
CCAAACA	30	1.0274616E-6	50.064445	37
AGTACCT	20	4.1360446E-4	50.06444	38
AAATACT	15	0.008381332	50.035656	35
AACGGCG	15	0.008381332	50.035656	36
GCCAAAC	30	1.0315525E-6	50.035656	36
TTCCTCG	15	0.008381332	50.035656	35
CTACTGT	25	2.0768186E-5	49.978176	32
ACCTCCG	15	0.008419526	49.978176	30
GCAACGA	15	0.008438671	49.949486	24
ATGGAGC	15	0.008438671	49.949486	26
GTGGCGA	15	0.008438671	49.949486	28
TCGGCGA	15	0.008438671	49.949486	24

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content