FastQCFastQC Report
Fri 17 Jun 2016
SRR1526754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123213
Sequences flagged as poor quality0
Sequence length64
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.4926428217801693No Hit
AACCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3240.2629592656619026No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2520.20452387329259086No Hit
AACCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.18179899848230302No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1730.14040726222070724No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1550.1257984141283793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTTA201.9098738E-458.562558
ATTCAGC150.00450183458.56249658
TAGGGTA1100.058.179745
GGAATCG150.004657803658.06114655
AAACCTC150.004665329658.03748748
TATGTCT150.004665329658.03748747
CGTAGAA202.0009105E-458.0138543
TACTAGC150.00467286558.01384735
CGAGACT150.00467286558.01384738
CGTCACA150.00467286558.01384737
CCCGACG150.00467286558.01384743
GGATATC258.632378E-657.990238
GGATTGC453.092282E-1157.990238
GGATTAC258.632378E-657.990238
GGGTCCG150.00468040957.9902278
GGATCGT150.00468040957.9902278
GGATCGA202.0049456E-457.9902278
GGCGCAA150.00468040957.9902278
GGATTGG407.1122486E-1057.9902278
GGATTCG150.00468040957.9902278
Produced by FastQC (version 0.11.5)