FastQCFastQC Report
Fri 17 Jun 2016
SRR1526753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166530
Sequences flagged as poor quality0
Sequence length64
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.5098180508016574No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC6900.4143397586020537No Hit
AACCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5560.33387377649672734No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC5460.32786885245901637No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4870.292439800636522No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4670.2804299525611001No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4300.2582117336215697No Hit
AACCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3060.18375067555395425No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT2920.17534378190115896No Hit
AACCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2580.15492704017294182No Hit
AACCTAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2570.1543265477691707No Hit
AACCTAGGGACCTCTGAACAGGGTACCCTTGTCCCCACCCCAGGCCCTGTTACCCTGTCCCTGC2350.1411157148862067No Hit
AACCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.13330931363718249No Hit
AACCTAGGGTAGCATTGGAACCAAACTCTTGCCCTGAGTTTCCAGTTGTTGGTAGGAACACAAT2000.12009848075421846No Hit
AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1960.1176965111391341No Hit
AACCTAGGGAAGCTAAAACAAGCAGAAAATGTATCTATCATGTTCCAACCCTGCATCCTCAGTG1860.11169158710142317No Hit
AACCTAGGGGACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGTCACTCTCC1830.10989010989010989No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1790.10748814027502553No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1680.1008827238335435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC150.00464183658.1206356
CGACCGA150.00464183658.1206356
CTAGTAG150.00464183658.1206358
GTCCAAC150.00464183658.1206358
TTCGACG201.9848466E-458.12062558
GTAATAC150.004647382558.1031148
CGGTACT201.9878088E-458.10310745
AAGTCCG351.6070771E-858.10310752
CCCTATA150.00465293458.08559442
CCCAATT150.00465293458.08559442
AGGTTAT150.00465293458.08559440
GCTTAAT150.00465293458.08559442
GCCCTAT150.00465293458.08559441
CACCCAA258.561921E-658.08559440
AGTAAGC150.00465293458.08559443
GCAATCT201.9967163E-458.05060637
GACGAGG150.004664052358.05060638
GTGCGAT150.00466961958.03312731
GTAACGA150.00466961958.03312730
CGTTAAA407.0940587E-1058.03312331
Produced by FastQC (version 0.11.5)