FastQCFastQC Report
Fri 17 Jun 2016
SRR1526752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165734
Sequences flagged as poor quality0
Sequence length64
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.48571807836653913No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC6130.3698697913524081No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC5330.3215996717631868No Hit
AACCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5080.30651525939155516No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4510.272122799184235No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4060.24497085691529802No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3760.22686956206934No Hit
AACCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3040.18342645443904088No Hit
AACCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.1617049006238913No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT2400.14481035876766385No Hit
AACCTAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2360.1423968527882028No Hit
AACCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2230.13455295835495432No Hit
AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2060.1242955579422448No Hit
AACCTAGGGACCTCTGAACAGGGTACCCTTGTCCCCACCCCAGGCCCTGTTACCCTGTCCCTGC2050.12369218144737955No Hit
AACCTAGGGTAGCATTGGAACCAAACTCTTGCCCTGAGTTTCCAGTTGTTGGTAGGAACACAAT1850.11162465155007421No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1750.10559088660142156No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1740.10498751010655628No Hit
AACCTAGGGGACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGTCACTCTCC1700.10257400412709522No Hit
AACCTAGGGACTTATATCCTCCCCAAAGACAGCCAGGTCGGGCCCGACGTGAAGTCCGAGGCGG1680.1013672511373647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTC201.931959E-458.43860626
GATACTC150.00455354558.40292744
GGCACGG258.2894E-658.40292741
TGCGAAA150.00455354558.40292744
CGATGTA150.00455354558.40292740
TGTCGTA303.5668927E-758.40292740
TGAGATA150.00455354558.40292742
GCATGCG453.092282E-1158.04854658
CGTATAG202.0060295E-457.99575851
TTGTAGG453.092282E-1157.99575847
CCGTATA202.0060295E-457.99575850
GTCATTC150.004681486657.99575450
GCCGCAT453.092282E-1157.97818455
CCCTGAT150.00469273757.96061737
TCACGGG150.00472660957.85545731
TCTTACC150.00472660957.85545731
GCTTATG150.00472660957.85545729
GTATCTA150.00472660957.85545731
CTAGGAT202.0332409E-457.837974
TAGAGGT150.00473227257.8379675
Produced by FastQC (version 0.11.5)