FastQCFastQC Report
Fri 17 Jun 2016
SRR1526751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147080
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC10790.7336143595322274No Hit
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9740.6622246396518902No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5960.40522164808267613No Hit
AACCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5770.39230350829480554No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4710.32023388632036986No Hit
AACCTAGGGACCTCTGAACAGGGTACCCTTGTCCCCACCCCAGGCCCTGTTACCCT3460.23524612455806362No Hit
AACCTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC3280.22300788686429154No Hit
AACCTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2960.20125101985314112No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2850.19377209681805818No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2300.15637748164264348No Hit
AACCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2300.15637748164264348No Hit
AACCTAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2240.15229806907805277No Hit
AACCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.14821865651346205No Hit
AACCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2170.14753875441936362No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2140.14549904813706827No Hit
AACCTAGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1900.12918139787870547No Hit
AACCTAGGGTAGCATTGGAACCAAACTCTTGCCCTGAGTTTCCAGTTGTTGGTAGG1650.1121838455262442No Hit
AACCTAGGGGTTCTTTATCGTTGGTGTCATTATAGGGAAGATTGCCTTGTAGGGGC1640.11150394343214577No Hit
AACCTAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1620.11014413924394888No Hit
AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1560.10606472667935817No Hit
AACCTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT1490.10130541202066903No Hit
AACCTAGGGATCATTCCTGCCTTTACGTGATTGGCTGGACGAGGGGGCAAAAGAAC1480.10062550992657056No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA203.8718607E-450.7613848
ACCGTAT203.8718607E-450.7613849
AACCGGT150.00792811350.76137548
TAGCGAG309.383675E-750.76137550
ACGGGAC150.00792811350.76137550
GTTACCC354.6573405E-850.76137549
CCGTATA251.9005716E-550.76137550
TCTAACT150.00792811350.76137549
CGACGGG150.00818156350.35906244
ACGTGAA150.00818156350.35906247
CGATGCA150.00818156350.35906247
CCAACCG150.00818156350.35906246
CGGTACT150.00818156350.35906245
ACGGTAC150.00818156350.35906244
AATAGGA150.00820388450.32438343
AGGTGTT150.00820388450.32438342
TAGGCTC402.5156623E-950.30705641
ACGTACT354.9996743E-850.30705641
ATACGTG252.0126594E-550.27244638
AGTACGA150.008237448550.27244638
Produced by FastQC (version 0.11.5)